Caracterização de dois begomovírus (Tomato severe rugose virus e Tomato yellow vein streak virus) que infectam tomateiro e obtenção de clones infecciosos

Detalhes bibliográficos
Autor(a) principal: Lima, Alison Talis Martins
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4367
Resumo: The genus Begomovirus (family Geminiviridae) includes viruses with a genome comprised of one or two molecules of circular, single-stranded DNA, transmitted to dicot species by the whitefly Bemisia tabaci. In Brazil, after the introduction of the B biotype of B. tabaci in the early 1990s, the incidence of begomoviruses in tomato has become frequent, with several reports of new viral species. Some of these species have become prevalent under field conditions, including Tomato severe rugose virus (ToSRV) and Tomato yellow vein streak virus (ToYVSV). The purpose of this study was to characterize two isolates of these species through the cloning of their whole genomes followed by molecular analysis, and the production of infectious clones for determination of their host ranges. Total DNA extracts of infected plants were used for whole genome amplification using the phi29 phage DNA polymerase. The amplification products were cloned into plasmids and completely sequenced. Sequence analysis indicated that the DNA-A and DNA-B of ToSRV-[BR:Pir1:05] and ToYVSV- [BR:Pda30:05] isolates had greater than 90% identities with other isolates of ToSRV and ToYVSV, respectively. The molecular analysis indicated that the DNA-A of the BR:Pir1:05 isolate may be the result of a recombination event, in which the virus acquired the rep ORF of Tomato rugose mosaic virus (ToRMV) and the cp ORF of an unidentified virus. Analysis of the DNA-B of the same isolate indicated the existence of a relationship with viruses that infect weed/wild hosts in Brazil, corroborating the hypothesis that viruses present in wild hosts led to the virus currently found in tomatoes. Additionally, because of the high identity observed between the DNA-B of ToSRV-[BR:Pir1:05] and ToRMV, it is possible that this genomic component is shared by the two viruses. In host range assays, plants showing latent infections were observed for both isolates. Such plants can act as natural reservoirs and serve as a primary source of inoculum for host plants of economic importance such as the tomato. The infectious clones of ToYVSV- [BR:Pda30:05] had low infectivity in the host range assays. It is possible that the inoculation method was not effective in the transmission of this isolate, or one or both clones could contain mutations that reduce the efficiency of their replication.
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spelling Lima, Alison Talis Martinshttp://lattes.cnpq.br/5848428341840005Zerbini, Poliane Alfenashttp://lattes.cnpq.br/8632328159533071Carvalho, Murilo Geraldo dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787250U5Zerbini Júnior, Francisco Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5Carvalho, Claudine Márciahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794965T6Barros, Danielle Ribeiro dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776125E2Lamêgo, Márcia Rogéria de Almeidahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782197P4Freitas, Leandro Grassi dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784897J62015-03-26T13:37:39Z2009-05-062015-03-26T13:37:39Z2008-07-30LIMA, Alison Talis Martins. Characterization of two begomoviruses (Tomato severe rugose virus and Tomato yellow vein streak virus) that infect tomato and production of infectious clones. 2008. 86 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/4367The genus Begomovirus (family Geminiviridae) includes viruses with a genome comprised of one or two molecules of circular, single-stranded DNA, transmitted to dicot species by the whitefly Bemisia tabaci. In Brazil, after the introduction of the B biotype of B. tabaci in the early 1990s, the incidence of begomoviruses in tomato has become frequent, with several reports of new viral species. Some of these species have become prevalent under field conditions, including Tomato severe rugose virus (ToSRV) and Tomato yellow vein streak virus (ToYVSV). The purpose of this study was to characterize two isolates of these species through the cloning of their whole genomes followed by molecular analysis, and the production of infectious clones for determination of their host ranges. Total DNA extracts of infected plants were used for whole genome amplification using the phi29 phage DNA polymerase. The amplification products were cloned into plasmids and completely sequenced. Sequence analysis indicated that the DNA-A and DNA-B of ToSRV-[BR:Pir1:05] and ToYVSV- [BR:Pda30:05] isolates had greater than 90% identities with other isolates of ToSRV and ToYVSV, respectively. The molecular analysis indicated that the DNA-A of the BR:Pir1:05 isolate may be the result of a recombination event, in which the virus acquired the rep ORF of Tomato rugose mosaic virus (ToRMV) and the cp ORF of an unidentified virus. Analysis of the DNA-B of the same isolate indicated the existence of a relationship with viruses that infect weed/wild hosts in Brazil, corroborating the hypothesis that viruses present in wild hosts led to the virus currently found in tomatoes. Additionally, because of the high identity observed between the DNA-B of ToSRV-[BR:Pir1:05] and ToRMV, it is possible that this genomic component is shared by the two viruses. In host range assays, plants showing latent infections were observed for both isolates. Such plants can act as natural reservoirs and serve as a primary source of inoculum for host plants of economic importance such as the tomato. The infectious clones of ToYVSV- [BR:Pda30:05] had low infectivity in the host range assays. It is possible that the inoculation method was not effective in the transmission of this isolate, or one or both clones could contain mutations that reduce the efficiency of their replication.O gênero Begomovirus (família Geminiviridae) inclui vírus com genoma composto por uma ou duas moléculas de DNA fita simples, transmitidos a espécies de plantas dicotiledôneas pela mosca-branca Bemisia tabaci. No Brasil, após a introdução do biótipo B de B. tabaci no início da década de 1990, a incidência de begomovírus em tomateiro tornou-se freqüente, com vários relatos de novas espécies virais. Algumas dessas espécies tornaram-se prevalentes em condições de campo no Brasil, incluindo o Tomato severe rugose virus (ToSRV) e o Tomato yellow vein streak virus (ToYVSV). O objetivo deste trabalho foi caracterizar dois isolados dessas espécies, por meio da clonagem de seus genomas completos seguida de análise molecular, e da obtenção de clones infecciosos para determinação de suas gamas de hospedeiros. Extratos de DNA total de plantas infectadas foram utilizados para a amplificação do genoma viral completo utilizando-se a DNA polimerase do fago φ29. Os produtos da amplificação foram clonados em plasmídeos e completamente seqüenciados. A análise das seqüências do DNA-A e DNA-B dos isolados ToSRV- [BR:Pir1:05] e ToYVSV- [BR:Pda30:05] indicou valores de identidade acima de 90% com outros isolados de ToSRV e ToYVSV, respectivamente. A análise molecular indica que o DNA-A do isolado BR:Pir1:05 pode ser resultado de um evento de recombinação, no qual o vírus adquiriu a ORF rep do Tomato rugose mosaic virus (ToRMV) e a ORF cp de um vírus não identificado. A análise do DNA-B do mesmo isolado indica a existência de relacionamento com vírus que infectam plantas daninhas/silvestres no Brasil, reforçando a hipótese de que vírus presentes em hospedeiros silvestres deram origem aos vírus atualmente encontrados em tomateiro. Adicionalmente, devido à alta identidade observada entre os DNAs-B dos isolados BR:Pir1:05 e do ToRMV, é possível que este componente genômico seja compartilhado pelos dois vírus. Nos testes de gama de hospedeiros, plantas apresentando infecções latentes foram observadas para os dois isolados. Tais plantas podem atuar como reservatórios naturais e servir de fonte de inóculo primário para plantas hospedeiras de importância econômica, como o tomateiro. Os clones infecciosos do isolado BR:Pda30:05 apresentaram baixa infectividade nos testes de gama de hospedeiros. É possível que o método de inoculação não tenha sido eficiente na transmissão deste isolado, ou que os clones apresentem mutações que reduzam a eficiência de sua replicação.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleTomateDoenças e pragasBegomovírusGeminivirusClonagem molecularTomatoPests and diseasesBegomovirusesGeminivirusesMolecular cloningCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIACaracterização de dois begomovírus (Tomato severe rugose virus e Tomato yellow vein streak virus) que infectam tomateiro e obtenção de clones infecciososCharacterization of two begomoviruses (Tomato severe rugose virus and Tomato yellow vein streak virus) that infect tomato and production of infectious clonesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf2474594https://locus.ufv.br//bitstream/123456789/4367/1/texto%20completo.pdf6cdab11f886d77e7d0c8a3d024dde318MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain134927https://locus.ufv.br//bitstream/123456789/4367/2/texto%20completo.pdf.txt7845d1d10eff94aa588e6305d39e54a5MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3652https://locus.ufv.br//bitstream/123456789/4367/3/texto%20completo.pdf.jpgf018dc6d319d6dc3a67c5c5438e1e084MD53123456789/43672016-04-10 23:08:32.867oai:locus.ufv.br:123456789/4367Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:08:32LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Caracterização de dois begomovírus (Tomato severe rugose virus e Tomato yellow vein streak virus) que infectam tomateiro e obtenção de clones infecciosos
dc.title.alternative.eng.fl_str_mv Characterization of two begomoviruses (Tomato severe rugose virus and Tomato yellow vein streak virus) that infect tomato and production of infectious clones
title Caracterização de dois begomovírus (Tomato severe rugose virus e Tomato yellow vein streak virus) que infectam tomateiro e obtenção de clones infecciosos
spellingShingle Caracterização de dois begomovírus (Tomato severe rugose virus e Tomato yellow vein streak virus) que infectam tomateiro e obtenção de clones infecciosos
Lima, Alison Talis Martins
Tomate
Doenças e pragas
Begomovírus
Geminivirus
Clonagem molecular
Tomato
Pests and diseases
Begomoviruses
Geminiviruses
Molecular cloning
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Caracterização de dois begomovírus (Tomato severe rugose virus e Tomato yellow vein streak virus) que infectam tomateiro e obtenção de clones infecciosos
title_full Caracterização de dois begomovírus (Tomato severe rugose virus e Tomato yellow vein streak virus) que infectam tomateiro e obtenção de clones infecciosos
title_fullStr Caracterização de dois begomovírus (Tomato severe rugose virus e Tomato yellow vein streak virus) que infectam tomateiro e obtenção de clones infecciosos
title_full_unstemmed Caracterização de dois begomovírus (Tomato severe rugose virus e Tomato yellow vein streak virus) que infectam tomateiro e obtenção de clones infecciosos
title_sort Caracterização de dois begomovírus (Tomato severe rugose virus e Tomato yellow vein streak virus) que infectam tomateiro e obtenção de clones infecciosos
author Lima, Alison Talis Martins
author_facet Lima, Alison Talis Martins
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/5848428341840005
dc.contributor.author.fl_str_mv Lima, Alison Talis Martins
dc.contributor.advisor-co1.fl_str_mv Zerbini, Poliane Alfenas
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/8632328159533071
dc.contributor.advisor-co2.fl_str_mv Carvalho, Murilo Geraldo de
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787250U5
dc.contributor.advisor1.fl_str_mv Zerbini Júnior, Francisco Murilo
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5
dc.contributor.referee1.fl_str_mv Carvalho, Claudine Márcia
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794965T6
dc.contributor.referee2.fl_str_mv Barros, Danielle Ribeiro de
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776125E2
dc.contributor.referee3.fl_str_mv Lamêgo, Márcia Rogéria de Almeida
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782197P4
dc.contributor.referee4.fl_str_mv Freitas, Leandro Grassi de
dc.contributor.referee4Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784897J6
contributor_str_mv Zerbini, Poliane Alfenas
Carvalho, Murilo Geraldo de
Zerbini Júnior, Francisco Murilo
Carvalho, Claudine Márcia
Barros, Danielle Ribeiro de
Lamêgo, Márcia Rogéria de Almeida
Freitas, Leandro Grassi de
dc.subject.por.fl_str_mv Tomate
Doenças e pragas
Begomovírus
Geminivirus
Clonagem molecular
topic Tomate
Doenças e pragas
Begomovírus
Geminivirus
Clonagem molecular
Tomato
Pests and diseases
Begomoviruses
Geminiviruses
Molecular cloning
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Tomato
Pests and diseases
Begomoviruses
Geminiviruses
Molecular cloning
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description The genus Begomovirus (family Geminiviridae) includes viruses with a genome comprised of one or two molecules of circular, single-stranded DNA, transmitted to dicot species by the whitefly Bemisia tabaci. In Brazil, after the introduction of the B biotype of B. tabaci in the early 1990s, the incidence of begomoviruses in tomato has become frequent, with several reports of new viral species. Some of these species have become prevalent under field conditions, including Tomato severe rugose virus (ToSRV) and Tomato yellow vein streak virus (ToYVSV). The purpose of this study was to characterize two isolates of these species through the cloning of their whole genomes followed by molecular analysis, and the production of infectious clones for determination of their host ranges. Total DNA extracts of infected plants were used for whole genome amplification using the phi29 phage DNA polymerase. The amplification products were cloned into plasmids and completely sequenced. Sequence analysis indicated that the DNA-A and DNA-B of ToSRV-[BR:Pir1:05] and ToYVSV- [BR:Pda30:05] isolates had greater than 90% identities with other isolates of ToSRV and ToYVSV, respectively. The molecular analysis indicated that the DNA-A of the BR:Pir1:05 isolate may be the result of a recombination event, in which the virus acquired the rep ORF of Tomato rugose mosaic virus (ToRMV) and the cp ORF of an unidentified virus. Analysis of the DNA-B of the same isolate indicated the existence of a relationship with viruses that infect weed/wild hosts in Brazil, corroborating the hypothesis that viruses present in wild hosts led to the virus currently found in tomatoes. Additionally, because of the high identity observed between the DNA-B of ToSRV-[BR:Pir1:05] and ToRMV, it is possible that this genomic component is shared by the two viruses. In host range assays, plants showing latent infections were observed for both isolates. Such plants can act as natural reservoirs and serve as a primary source of inoculum for host plants of economic importance such as the tomato. The infectious clones of ToYVSV- [BR:Pda30:05] had low infectivity in the host range assays. It is possible that the inoculation method was not effective in the transmission of this isolate, or one or both clones could contain mutations that reduce the efficiency of their replication.
publishDate 2008
dc.date.issued.fl_str_mv 2008-07-30
dc.date.available.fl_str_mv 2009-05-06
2015-03-26T13:37:39Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:37:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Alison Talis Martins. Characterization of two begomoviruses (Tomato severe rugose virus and Tomato yellow vein streak virus) that infect tomato and production of infectious clones. 2008. 86 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4367
identifier_str_mv LIMA, Alison Talis Martins. Characterization of two begomoviruses (Tomato severe rugose virus and Tomato yellow vein streak virus) that infect tomato and production of infectious clones. 2008. 86 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.
url http://locus.ufv.br/handle/123456789/4367
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dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.publisher.program.fl_str_mv Mestrado em Fitopatologia
dc.publisher.initials.fl_str_mv UFV
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Etiologia; Epidemiologia; Controle
publisher.none.fl_str_mv Universidade Federal de Viçosa
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