Imidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearum

Detalhes bibliográficos
Autor(a) principal: Ribas, A.D.
Data de Publicação: 2016
Outros Autores: Del Ponte, E.M., Dalbem, A.M., Dalla-Lana, D., Bündchen, C., Donato, R.K., Schrekker, H.S., Fuentefria, A.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1111/jam.13125
http://www.locus.ufv.br/handle/123456789/18758
Resumo: Evaluate the in vitro effect of imidazolium salts (IMS) on the conidia germination and mycelial growth of Fusarium graminearum and their in vivo efficacy for suppressing the symptoms of the disease and infection of kernels in wheat plants. The minimum inhibitory concentrations (MIC) of three IMS (C 16 MImCl, C 16 MImMeS and C 16 MImNTf 2 ) were determined for four F. graminearum isolates using serial broth dilution method. The MICs found for all IMS were either 3 12 or 6 25 lg ml 1 across the isolates, with the former as the most frequent. In the mycelial growth assay on potato dextrose agar media, only the C 16 MImCl among the IMS reduced 50% of mycelial growth of one isolate at an estimated concentration of 0 32 mg ml 1 . The time-kill curves showed a strong fungicidal effect starting 1 h after incubation at a concentration of 12 5 lg ml 1 , representing a fourfold increase in the most frequent MIC. The C 16 MImCl sprayed onto the spikes of potted wheat plants during the flowering stage reduced disease intensity at levels comparable to the commercial fungicide when applied preventatively (1 h prior to fungal inoculation), rather than curatively, and at the higher dosage (2 mg ml 1 ) rather than lower dosage (0 5 mg ml 1 ). C 16 MImCl proved to be a potent inhibitor of F. graminearum growth and provided good levels of control of the disease at levels comparable to a commercial fungicide, in wheat plants treated prior to fungal infection during flowering stages.This study suggests the potential of using IMS as alternative to the hazardous standard fungicides in the management of Fusarium head blight of wheat.
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spelling Ribas, A.D.Del Ponte, E.M.Dalbem, A.M.Dalla-Lana, D.Bündchen, C.Donato, R.K.Schrekker, H.S.Fuentefria, A.M.2018-04-17T14:17:42Z2018-04-17T14:17:42Z2016-03-0713645072https://doi.org/10.1111/jam.13125http://www.locus.ufv.br/handle/123456789/18758Evaluate the in vitro effect of imidazolium salts (IMS) on the conidia germination and mycelial growth of Fusarium graminearum and their in vivo efficacy for suppressing the symptoms of the disease and infection of kernels in wheat plants. The minimum inhibitory concentrations (MIC) of three IMS (C 16 MImCl, C 16 MImMeS and C 16 MImNTf 2 ) were determined for four F. graminearum isolates using serial broth dilution method. The MICs found for all IMS were either 3 12 or 6 25 lg ml 1 across the isolates, with the former as the most frequent. In the mycelial growth assay on potato dextrose agar media, only the C 16 MImCl among the IMS reduced 50% of mycelial growth of one isolate at an estimated concentration of 0 32 mg ml 1 . The time-kill curves showed a strong fungicidal effect starting 1 h after incubation at a concentration of 12 5 lg ml 1 , representing a fourfold increase in the most frequent MIC. The C 16 MImCl sprayed onto the spikes of potted wheat plants during the flowering stage reduced disease intensity at levels comparable to the commercial fungicide when applied preventatively (1 h prior to fungal inoculation), rather than curatively, and at the higher dosage (2 mg ml 1 ) rather than lower dosage (0 5 mg ml 1 ). C 16 MImCl proved to be a potent inhibitor of F. graminearum growth and provided good levels of control of the disease at levels comparable to a commercial fungicide, in wheat plants treated prior to fungal infection during flowering stages.This study suggests the potential of using IMS as alternative to the hazardous standard fungicides in the management of Fusarium head blight of wheat.engJournal of Applied Microbiologyv. 121, Issue 2, p. 445-452, August 2016The Society for Applied Microbiologyinfo:eu-repo/semantics/openAccessFungicidesFusarium graminearumImidazolium saltsInhibitionWheatImidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf595261https://locus.ufv.br//bitstream/123456789/18758/1/artigo.pdf4147785a3a5e5e0e024c9bad025599f1MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/18758/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5729https://locus.ufv.br//bitstream/123456789/18758/3/artigo.pdf.jpg3c98dd57a06b7b0f9b69c2119a15f7a9MD53123456789/187582018-04-17 23:00:49.855oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-18T02:00:49LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Imidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearum
title Imidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearum
spellingShingle Imidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearum
Ribas, A.D.
Fungicides
Fusarium graminearum
Imidazolium salts
Inhibition
Wheat
title_short Imidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearum
title_full Imidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearum
title_fullStr Imidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearum
title_full_unstemmed Imidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearum
title_sort Imidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearum
author Ribas, A.D.
author_facet Ribas, A.D.
Del Ponte, E.M.
Dalbem, A.M.
Dalla-Lana, D.
Bündchen, C.
Donato, R.K.
Schrekker, H.S.
Fuentefria, A.M.
author_role author
author2 Del Ponte, E.M.
Dalbem, A.M.
Dalla-Lana, D.
Bündchen, C.
Donato, R.K.
Schrekker, H.S.
Fuentefria, A.M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ribas, A.D.
Del Ponte, E.M.
Dalbem, A.M.
Dalla-Lana, D.
Bündchen, C.
Donato, R.K.
Schrekker, H.S.
Fuentefria, A.M.
dc.subject.pt-BR.fl_str_mv Fungicides
Fusarium graminearum
Imidazolium salts
Inhibition
Wheat
topic Fungicides
Fusarium graminearum
Imidazolium salts
Inhibition
Wheat
description Evaluate the in vitro effect of imidazolium salts (IMS) on the conidia germination and mycelial growth of Fusarium graminearum and their in vivo efficacy for suppressing the symptoms of the disease and infection of kernels in wheat plants. The minimum inhibitory concentrations (MIC) of three IMS (C 16 MImCl, C 16 MImMeS and C 16 MImNTf 2 ) were determined for four F. graminearum isolates using serial broth dilution method. The MICs found for all IMS were either 3 12 or 6 25 lg ml 1 across the isolates, with the former as the most frequent. In the mycelial growth assay on potato dextrose agar media, only the C 16 MImCl among the IMS reduced 50% of mycelial growth of one isolate at an estimated concentration of 0 32 mg ml 1 . The time-kill curves showed a strong fungicidal effect starting 1 h after incubation at a concentration of 12 5 lg ml 1 , representing a fourfold increase in the most frequent MIC. The C 16 MImCl sprayed onto the spikes of potted wheat plants during the flowering stage reduced disease intensity at levels comparable to the commercial fungicide when applied preventatively (1 h prior to fungal inoculation), rather than curatively, and at the higher dosage (2 mg ml 1 ) rather than lower dosage (0 5 mg ml 1 ). C 16 MImCl proved to be a potent inhibitor of F. graminearum growth and provided good levels of control of the disease at levels comparable to a commercial fungicide, in wheat plants treated prior to fungal infection during flowering stages.This study suggests the potential of using IMS as alternative to the hazardous standard fungicides in the management of Fusarium head blight of wheat.
publishDate 2016
dc.date.issued.fl_str_mv 2016-03-07
dc.date.accessioned.fl_str_mv 2018-04-17T14:17:42Z
dc.date.available.fl_str_mv 2018-04-17T14:17:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://doi.org/10.1111/jam.13125
http://www.locus.ufv.br/handle/123456789/18758
dc.identifier.issn.none.fl_str_mv 13645072
identifier_str_mv 13645072
url https://doi.org/10.1111/jam.13125
http://www.locus.ufv.br/handle/123456789/18758
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 121, Issue 2, p. 445-452, August 2016
dc.rights.driver.fl_str_mv The Society for Applied Microbiology
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