Avaliações fisiológicas, bioquímicas e histoquímica de Ipomoea pes-caprae cultivada em diferentes concentrações de ferro

Detalhes bibliográficos
Autor(a) principal: Poelking, Viviane Guzzo de Carli
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/2509
Resumo: The aim of this study was to evaluate the effects of excess iron over Ipomoea pescaprae, a native species common to Restinga. Saplings presenting three pairs of leaves were grown under 2, 3, 4 and 5 mM Fe-EDTA concentrations in Hoagland solution at half strength. After seven days, it was evaluated the accumulation of iron in plant tissues, alterations in the content of chlorophyll a, chlorophyll b, carotenoids, in fluorescence of chlorophyll a and gas exchanges parameters and the development of oxidative stress. After 48 hours there were visual symptoms on leaves. The iron was absorbed and accumulated in roots and leaves, reaching phytotoxic values under the lowest iron concentration. The accumulation sites of iron were identified hystochemicaly in roots and leaves tissues. Significant alterations were observed for fluorescence parameters, for enzymatic activity of peroxidase, superoxide dismutase and ascorbato peroxidase and malondialdehyde concentration, indicating the development of oxidative stress. There were also significant reductions of 67.3% in the content of chlorophyll a, 64.0% in chlorophyll b and 48.9% in carotenoids. The alterations observed in chlorophyll content, as well as in fluorescence of chlorophyll a parameters, were obtained at concentrations above of 2 mM Fe-EDTA. On the other hand, gas exchanges parameters were affected under all iron concentrations. The assimilation of CO2 decreased 87%, the stomatal conductance 63% and the ratio Ci/Ca decreased 22%. The 50% reduction of photosynthesis was reached at lower concentration, in comparison to stomatal conductance, chlorophyll content and electron transport rate, which presented reductions at higher concentrations. It can be concluded that Ipomoea pes-caprae showed to be sensitive to iron in concentrations above 2 mM and that the parameters evaluated in this studies may be potential tools as biomarkers of environmental hazards.
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spelling Poelking, Viviane Guzzo de Carlihttp://lattes.cnpq.br/5568088478803341Silva, Luzimar Campos dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799707J8Almeida, Andréa Miyasaka dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792501H4Cano, Marco Antonio Olivahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787546T4Ribas, Rogério Ferreirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4777511A8Kuki, Kacilda Naomihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784674P62015-03-26T13:08:44Z2009-09-022015-03-26T13:08:44Z2008-07-11POELKING, Viviane Guzzo de Carli. Physiological, biochemical and histochemical evaluations of Ipomoea pes-caprae cultivated at different concentrations of iron. 2008. 3 f. Dissertação (Mestrado em Botânica estrutural; Ecologia e Sistemática) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/2509The aim of this study was to evaluate the effects of excess iron over Ipomoea pescaprae, a native species common to Restinga. Saplings presenting three pairs of leaves were grown under 2, 3, 4 and 5 mM Fe-EDTA concentrations in Hoagland solution at half strength. After seven days, it was evaluated the accumulation of iron in plant tissues, alterations in the content of chlorophyll a, chlorophyll b, carotenoids, in fluorescence of chlorophyll a and gas exchanges parameters and the development of oxidative stress. After 48 hours there were visual symptoms on leaves. The iron was absorbed and accumulated in roots and leaves, reaching phytotoxic values under the lowest iron concentration. The accumulation sites of iron were identified hystochemicaly in roots and leaves tissues. Significant alterations were observed for fluorescence parameters, for enzymatic activity of peroxidase, superoxide dismutase and ascorbato peroxidase and malondialdehyde concentration, indicating the development of oxidative stress. There were also significant reductions of 67.3% in the content of chlorophyll a, 64.0% in chlorophyll b and 48.9% in carotenoids. The alterations observed in chlorophyll content, as well as in fluorescence of chlorophyll a parameters, were obtained at concentrations above of 2 mM Fe-EDTA. On the other hand, gas exchanges parameters were affected under all iron concentrations. The assimilation of CO2 decreased 87%, the stomatal conductance 63% and the ratio Ci/Ca decreased 22%. The 50% reduction of photosynthesis was reached at lower concentration, in comparison to stomatal conductance, chlorophyll content and electron transport rate, which presented reductions at higher concentrations. It can be concluded that Ipomoea pes-caprae showed to be sensitive to iron in concentrations above 2 mM and that the parameters evaluated in this studies may be potential tools as biomarkers of environmental hazards.Plantas de Ipomoea pes-caprae, espécie da restinga, foram cultivas com objetivo de avaliar os efeitos da absorção de ferro quando oferecido em excesso. Plantas com três pares de folhas foram submetidas a doses de 2, 3, 4 e 5mM de Fe- EDTA em solução de Hoanglad meia força. Após sete dias foram avaliados o acúmulo de ferro nos tecidos, as alterações no teor de clorofila a, clorofila b, carotenóides, a fluorescência da clorofila a, as trocas gasosas e o estresse oxidativos. Ocorreram sintomas visuais na lamina foliar 48h após aplicação do tratamento. O ferro foi absorvido e acumulado nas raízes e folhas, alcançando valores fitotóxicos na primeira dose. O acúmulo de ferro foi localizado histoquimicamente em todos os tecidos das raízes e folhas. Ocorreram alterações significativas nos valores de fluorescência da clorofila a, na atividade enzimática da superóxido dismutase, peroxidases, e peroxidase do ascorbato e nas concentrações de malonaldeído, o que indica a ocorrência de estresse oxidativo. Foram verificas reduções significativas de 67,3% da clorofila a, 64% da clorofila b e 48,9% dos carotenóides. As alterações do teor de clorofila e fluorescência da clorofila a tiveram início em doses superiores a 2mM. As trocas gasosas foram alteradas em todas as concentrações de ferro, a fotossíntese reduziu 87%, a condutância estomática 63% e a razão Ci/Ca obteve acréscimo de 22%. A redução da fotossíntese em 50% ocorreu com menor quantidade de ferro total nas folhas quando comparada a condutância estomática, teor de clorofila a e taxa de transporte de elétrons, que apresentaram redução com dose de ferro nas folhas superior a necessária para reduzir a fotossíntese. É possível inferir que, Ipomoea pes-caprae mostrou-se susceptível ao ferro nas doses superiores a 2 mM e a sensibilidade dos parâmetros avaliados podem ser potencias biomarcadores de risco ambiental.application/pdfporUniversidade Federal de ViçosaMestrado em BotânicaUFVBRBotânica estrutural; Ecologia e SistemáticaIpomoea pes-capraeFerroToxidezIpomoea pes-capraeIronToxicityCNPQ::CIENCIAS BIOLOGICAS::BOTANICAAvaliações fisiológicas, bioquímicas e histoquímica de Ipomoea pes-caprae cultivada em diferentes concentrações de ferroPhysiological, biochemical and histochemical evaluations of Ipomoea pes-caprae cultivated at different concentrations of ironinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL01 - capa_abstract.pdfapplication/pdf19725https://locus.ufv.br//bitstream/123456789/2509/1/01%20-%20capa_abstract.pdfd43f6bb6faac172c125d1dd4b5080b05MD51TEXT01 - capa_abstract.pdf.txt01 - capa_abstract.pdf.txtExtracted texttext/plain4889https://locus.ufv.br//bitstream/123456789/2509/2/01%20-%20capa_abstract.pdf.txt9f454c4b6b09fa503a0f22a48528be7bMD52THUMBNAIL01 - capa_abstract.pdf.jpg01 - capa_abstract.pdf.jpgIM Thumbnailimage/jpeg3463https://locus.ufv.br//bitstream/123456789/2509/3/01%20-%20capa_abstract.pdf.jpgaf1bb5b7afa484a31075507edf13bbb5MD53123456789/25092016-04-08 23:08:10.836oai:locus.ufv.br:123456789/2509Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-09T02:08:10LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Avaliações fisiológicas, bioquímicas e histoquímica de Ipomoea pes-caprae cultivada em diferentes concentrações de ferro
dc.title.alternative.eng.fl_str_mv Physiological, biochemical and histochemical evaluations of Ipomoea pes-caprae cultivated at different concentrations of iron
title Avaliações fisiológicas, bioquímicas e histoquímica de Ipomoea pes-caprae cultivada em diferentes concentrações de ferro
spellingShingle Avaliações fisiológicas, bioquímicas e histoquímica de Ipomoea pes-caprae cultivada em diferentes concentrações de ferro
Poelking, Viviane Guzzo de Carli
Ipomoea pes-caprae
Ferro
Toxidez
Ipomoea pes-caprae
Iron
Toxicity
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA
title_short Avaliações fisiológicas, bioquímicas e histoquímica de Ipomoea pes-caprae cultivada em diferentes concentrações de ferro
title_full Avaliações fisiológicas, bioquímicas e histoquímica de Ipomoea pes-caprae cultivada em diferentes concentrações de ferro
title_fullStr Avaliações fisiológicas, bioquímicas e histoquímica de Ipomoea pes-caprae cultivada em diferentes concentrações de ferro
title_full_unstemmed Avaliações fisiológicas, bioquímicas e histoquímica de Ipomoea pes-caprae cultivada em diferentes concentrações de ferro
title_sort Avaliações fisiológicas, bioquímicas e histoquímica de Ipomoea pes-caprae cultivada em diferentes concentrações de ferro
author Poelking, Viviane Guzzo de Carli
author_facet Poelking, Viviane Guzzo de Carli
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/5568088478803341
dc.contributor.author.fl_str_mv Poelking, Viviane Guzzo de Carli
dc.contributor.advisor-co1.fl_str_mv Silva, Luzimar Campos da
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799707J8
dc.contributor.advisor-co2.fl_str_mv Almeida, Andréa Miyasaka de
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792501H4
dc.contributor.advisor1.fl_str_mv Cano, Marco Antonio Oliva
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787546T4
dc.contributor.referee1.fl_str_mv Ribas, Rogério Ferreira
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4777511A8
dc.contributor.referee2.fl_str_mv Kuki, Kacilda Naomi
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784674P6
contributor_str_mv Silva, Luzimar Campos da
Almeida, Andréa Miyasaka de
Cano, Marco Antonio Oliva
Ribas, Rogério Ferreira
Kuki, Kacilda Naomi
dc.subject.por.fl_str_mv Ipomoea pes-caprae
Ferro
Toxidez
topic Ipomoea pes-caprae
Ferro
Toxidez
Ipomoea pes-caprae
Iron
Toxicity
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA
dc.subject.eng.fl_str_mv Ipomoea pes-caprae
Iron
Toxicity
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BOTANICA
description The aim of this study was to evaluate the effects of excess iron over Ipomoea pescaprae, a native species common to Restinga. Saplings presenting three pairs of leaves were grown under 2, 3, 4 and 5 mM Fe-EDTA concentrations in Hoagland solution at half strength. After seven days, it was evaluated the accumulation of iron in plant tissues, alterations in the content of chlorophyll a, chlorophyll b, carotenoids, in fluorescence of chlorophyll a and gas exchanges parameters and the development of oxidative stress. After 48 hours there were visual symptoms on leaves. The iron was absorbed and accumulated in roots and leaves, reaching phytotoxic values under the lowest iron concentration. The accumulation sites of iron were identified hystochemicaly in roots and leaves tissues. Significant alterations were observed for fluorescence parameters, for enzymatic activity of peroxidase, superoxide dismutase and ascorbato peroxidase and malondialdehyde concentration, indicating the development of oxidative stress. There were also significant reductions of 67.3% in the content of chlorophyll a, 64.0% in chlorophyll b and 48.9% in carotenoids. The alterations observed in chlorophyll content, as well as in fluorescence of chlorophyll a parameters, were obtained at concentrations above of 2 mM Fe-EDTA. On the other hand, gas exchanges parameters were affected under all iron concentrations. The assimilation of CO2 decreased 87%, the stomatal conductance 63% and the ratio Ci/Ca decreased 22%. The 50% reduction of photosynthesis was reached at lower concentration, in comparison to stomatal conductance, chlorophyll content and electron transport rate, which presented reductions at higher concentrations. It can be concluded that Ipomoea pes-caprae showed to be sensitive to iron in concentrations above 2 mM and that the parameters evaluated in this studies may be potential tools as biomarkers of environmental hazards.
publishDate 2008
dc.date.issued.fl_str_mv 2008-07-11
dc.date.available.fl_str_mv 2009-09-02
2015-03-26T13:08:44Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:08:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv POELKING, Viviane Guzzo de Carli. Physiological, biochemical and histochemical evaluations of Ipomoea pes-caprae cultivated at different concentrations of iron. 2008. 3 f. Dissertação (Mestrado em Botânica estrutural; Ecologia e Sistemática) - Universidade Federal de Viçosa, Viçosa, 2008.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/2509
identifier_str_mv POELKING, Viviane Guzzo de Carli. Physiological, biochemical and histochemical evaluations of Ipomoea pes-caprae cultivated at different concentrations of iron. 2008. 3 f. Dissertação (Mestrado em Botânica estrutural; Ecologia e Sistemática) - Universidade Federal de Viçosa, Viçosa, 2008.
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dc.publisher.department.fl_str_mv Botânica estrutural; Ecologia e Sistemática
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