Impaired cyclic electron flow around photosystem I disturbs high-light respiratory metabolism
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1104/pp.16.01025 http://www.locus.ufv.br/handle/123456789/15011 |
Resumo: | The cyclic electron flow around photosystem I (CEF-PSI) increases ATP/NADPH production in the chloroplast, acting as an energy balance mechanism. Higher export of reducing power from the chloroplast in CEF-PSI mutants has been correlated with higher mitochondrial alternative oxidase (AOX) capacity and protein amount under high-light (HL) conditions. However, in vivo measurements of AOX activity are still required to confirm the exact role of AOX in dissipating the excess of reductant power from the chloroplast. Here, CEF-PSI single and double mutants were exposed to short-term HL conditions in Arabidopsis (Arabidopsis thaliana). Chlorophyll fluorescence, in vivo activities of the cytochrome oxidase (νcyt) and AOX (νalt) pathways, levels of mitochondrial proteins, metabolite profiles, and pyridine nucleotide levels were determined under normal growth and HL conditions. νalt was not increased in CEF-PSI mutants, while AOX capacity was positively correlated with photoinhibition, probably due to a reactive oxygen species-induced increase of AOX protein. The severe metabolic impairment observed in CEF-PSI mutants, as indicated by the increase in photoinhibition and changes in the levels of stress-related metabolites, can explain their lack of νalt induction. By contrast, νcyt was positively correlated with photosynthetic performance. Correlations with metabolite changes suggest that νcyt is coordinated with sugar metabolism and stress-related amino acid synthesis. Furthermore, changes in glycine-serine and NADH-NAD+ ratios were highly correlated to νcyt Taken together, our results suggest that νcyt can act as a sink for the excess of electrons from the chloroplast, probably via photorespiratory glycine oxidation, thus improving photosynthetic performance when νalt is not induced under severe HL stress. |
id |
UFV_df3809f535b50140fed88c5c2f86724c |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/15011 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
2145 |
spelling |
Florez-Sarasa, IgorNoguchi, KoAraújo, Wagner L.Garcia-Nogales, AnaFernie, Alisdair R.Flexas, JaumeRibas-Carbo, Miquel2017-12-14T10:12:14Z2017-12-14T10:12:14Z2016-120176-1617https://doi.org/10.1104/pp.16.01025http://www.locus.ufv.br/handle/123456789/15011The cyclic electron flow around photosystem I (CEF-PSI) increases ATP/NADPH production in the chloroplast, acting as an energy balance mechanism. Higher export of reducing power from the chloroplast in CEF-PSI mutants has been correlated with higher mitochondrial alternative oxidase (AOX) capacity and protein amount under high-light (HL) conditions. However, in vivo measurements of AOX activity are still required to confirm the exact role of AOX in dissipating the excess of reductant power from the chloroplast. Here, CEF-PSI single and double mutants were exposed to short-term HL conditions in Arabidopsis (Arabidopsis thaliana). Chlorophyll fluorescence, in vivo activities of the cytochrome oxidase (νcyt) and AOX (νalt) pathways, levels of mitochondrial proteins, metabolite profiles, and pyridine nucleotide levels were determined under normal growth and HL conditions. νalt was not increased in CEF-PSI mutants, while AOX capacity was positively correlated with photoinhibition, probably due to a reactive oxygen species-induced increase of AOX protein. The severe metabolic impairment observed in CEF-PSI mutants, as indicated by the increase in photoinhibition and changes in the levels of stress-related metabolites, can explain their lack of νalt induction. By contrast, νcyt was positively correlated with photosynthetic performance. Correlations with metabolite changes suggest that νcyt is coordinated with sugar metabolism and stress-related amino acid synthesis. Furthermore, changes in glycine-serine and NADH-NAD+ ratios were highly correlated to νcyt Taken together, our results suggest that νcyt can act as a sink for the excess of electrons from the chloroplast, probably via photorespiratory glycine oxidation, thus improving photosynthetic performance when νalt is not induced under severe HL stress.engPlant PhysiologyVol. 172, n. 4, p. 2176–2189, Dec. 2016Photosystem IRespiratory metabolismImpaired cyclic electron flow around photosystem I disturbs high-light respiratory metabolisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL2176.full.pdf2176.full.pdftexto completoapplication/pdf1118147https://locus.ufv.br//bitstream/123456789/15011/1/2176.full.pdf80c54920fbd3e53fcf115fdf4089110cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/15011/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAIL2176.full.pdf.jpg2176.full.pdf.jpgIM Thumbnailimage/jpeg5207https://locus.ufv.br//bitstream/123456789/15011/3/2176.full.pdf.jpg9f07735d6d019e97a8b18f0e216cbce5MD53123456789/150112017-12-14 22:00:42.126oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-12-15T01:00:42LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Impaired cyclic electron flow around photosystem I disturbs high-light respiratory metabolism |
title |
Impaired cyclic electron flow around photosystem I disturbs high-light respiratory metabolism |
spellingShingle |
Impaired cyclic electron flow around photosystem I disturbs high-light respiratory metabolism Florez-Sarasa, Igor Photosystem I Respiratory metabolism |
title_short |
Impaired cyclic electron flow around photosystem I disturbs high-light respiratory metabolism |
title_full |
Impaired cyclic electron flow around photosystem I disturbs high-light respiratory metabolism |
title_fullStr |
Impaired cyclic electron flow around photosystem I disturbs high-light respiratory metabolism |
title_full_unstemmed |
Impaired cyclic electron flow around photosystem I disturbs high-light respiratory metabolism |
title_sort |
Impaired cyclic electron flow around photosystem I disturbs high-light respiratory metabolism |
author |
Florez-Sarasa, Igor |
author_facet |
Florez-Sarasa, Igor Noguchi, Ko Araújo, Wagner L. Garcia-Nogales, Ana Fernie, Alisdair R. Flexas, Jaume Ribas-Carbo, Miquel |
author_role |
author |
author2 |
Noguchi, Ko Araújo, Wagner L. Garcia-Nogales, Ana Fernie, Alisdair R. Flexas, Jaume Ribas-Carbo, Miquel |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Florez-Sarasa, Igor Noguchi, Ko Araújo, Wagner L. Garcia-Nogales, Ana Fernie, Alisdair R. Flexas, Jaume Ribas-Carbo, Miquel |
dc.subject.pt-BR.fl_str_mv |
Photosystem I Respiratory metabolism |
topic |
Photosystem I Respiratory metabolism |
description |
The cyclic electron flow around photosystem I (CEF-PSI) increases ATP/NADPH production in the chloroplast, acting as an energy balance mechanism. Higher export of reducing power from the chloroplast in CEF-PSI mutants has been correlated with higher mitochondrial alternative oxidase (AOX) capacity and protein amount under high-light (HL) conditions. However, in vivo measurements of AOX activity are still required to confirm the exact role of AOX in dissipating the excess of reductant power from the chloroplast. Here, CEF-PSI single and double mutants were exposed to short-term HL conditions in Arabidopsis (Arabidopsis thaliana). Chlorophyll fluorescence, in vivo activities of the cytochrome oxidase (νcyt) and AOX (νalt) pathways, levels of mitochondrial proteins, metabolite profiles, and pyridine nucleotide levels were determined under normal growth and HL conditions. νalt was not increased in CEF-PSI mutants, while AOX capacity was positively correlated with photoinhibition, probably due to a reactive oxygen species-induced increase of AOX protein. The severe metabolic impairment observed in CEF-PSI mutants, as indicated by the increase in photoinhibition and changes in the levels of stress-related metabolites, can explain their lack of νalt induction. By contrast, νcyt was positively correlated with photosynthetic performance. Correlations with metabolite changes suggest that νcyt is coordinated with sugar metabolism and stress-related amino acid synthesis. Furthermore, changes in glycine-serine and NADH-NAD+ ratios were highly correlated to νcyt Taken together, our results suggest that νcyt can act as a sink for the excess of electrons from the chloroplast, probably via photorespiratory glycine oxidation, thus improving photosynthetic performance when νalt is not induced under severe HL stress. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-12 |
dc.date.accessioned.fl_str_mv |
2017-12-14T10:12:14Z |
dc.date.available.fl_str_mv |
2017-12-14T10:12:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1104/pp.16.01025 http://www.locus.ufv.br/handle/123456789/15011 |
dc.identifier.issn.none.fl_str_mv |
0176-1617 |
identifier_str_mv |
0176-1617 |
url |
https://doi.org/10.1104/pp.16.01025 http://www.locus.ufv.br/handle/123456789/15011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Vol. 172, n. 4, p. 2176–2189, Dec. 2016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Plant Physiology |
publisher.none.fl_str_mv |
Plant Physiology |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/15011/1/2176.full.pdf https://locus.ufv.br//bitstream/123456789/15011/2/license.txt https://locus.ufv.br//bitstream/123456789/15011/3/2176.full.pdf.jpg |
bitstream.checksum.fl_str_mv |
80c54920fbd3e53fcf115fdf4089110c 8a4605be74aa9ea9d79846c1fba20a33 9f07735d6d019e97a8b18f0e216cbce5 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1801213117759225856 |