Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus

Detalhes bibliográficos
Autor(a) principal: Araújo, J.V.
Data de Publicação: 1997
Outros Autores: Junghans, T.G., Alfenas, A.C., Gomes, A.P.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S0100-879X1997001000001
http://www.locus.ufv.br/handle/123456789/11962
Resumo: Extraction and isoenzyme analysis of four isolates of rthrobotrys including A. musiformis, A. robusta and A. conoides were conducted. Among the 14 enzymes studied by starch gel electrophoresis, using morpholine-citrate as gel/electrode buffer, the following nine enzymes showed interpretable banding patterns: α-esterase, fumarase, hexokinase, isocitrate dehydrogenase, leucine aminopeptidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphogluco- mutase and phosphoglucoisomerase. All isolates studied displayed typical isoenzyme phenotypes for each species. Two isolates of A. conoides differed in their α-isoesterase banding patterns, but no differences were observed for the other enzymes. The assay was satisfactory for enzyme extraction and resolution of Arthrobotrys and could be used in future taxonomic and genetic studies of this organism.
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spelling Araújo, J.V.Junghans, T.G.Alfenas, A.C.Gomes, A.P.S.2017-10-09T18:32:34Z2017-10-09T18:32:34Z1997-06-281414-431Xhttp://dx.doi.org/10.1590/S0100-879X1997001000001http://www.locus.ufv.br/handle/123456789/11962Extraction and isoenzyme analysis of four isolates of rthrobotrys including A. musiformis, A. robusta and A. conoides were conducted. Among the 14 enzymes studied by starch gel electrophoresis, using morpholine-citrate as gel/electrode buffer, the following nine enzymes showed interpretable banding patterns: α-esterase, fumarase, hexokinase, isocitrate dehydrogenase, leucine aminopeptidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphogluco- mutase and phosphoglucoisomerase. All isolates studied displayed typical isoenzyme phenotypes for each species. Two isolates of A. conoides differed in their α-isoesterase banding patterns, but no differences were observed for the other enzymes. The assay was satisfactory for enzyme extraction and resolution of Arthrobotrys and could be used in future taxonomic and genetic studies of this organism.engBrazilian Journal of Medical and Biological Researchvol. 30 n. 10 1149-1152 Oct. 1997Nematophagous fungiNematode-trapping fungusPredacious fungiArthrobotrysIsoenzymesElectrophoresisIsoenzyme analysis of Arthrobotrys, a nematode-trapping fungusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL2849c.pdf2849c.pdftexto completoapplication/pdf189068https://locus.ufv.br//bitstream/123456789/11962/1/2849c.pdf7771b931dd9f4d716ce9779281c87782MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/11962/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAIL2849c.pdf.jpg2849c.pdf.jpgIM Thumbnailimage/jpeg4373https://locus.ufv.br//bitstream/123456789/11962/3/2849c.pdf.jpg1c879df478059887b7184f8f0c6af2faMD53123456789/119622018-04-17 11:04:27.603oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-17T14:04:27LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
title Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
spellingShingle Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
Araújo, J.V.
Nematophagous fungi
Nematode-trapping fungus
Predacious fungi
Arthrobotrys
Isoenzymes
Electrophoresis
title_short Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
title_full Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
title_fullStr Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
title_full_unstemmed Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
title_sort Isoenzyme analysis of Arthrobotrys, a nematode-trapping fungus
author Araújo, J.V.
author_facet Araújo, J.V.
Junghans, T.G.
Alfenas, A.C.
Gomes, A.P.S.
author_role author
author2 Junghans, T.G.
Alfenas, A.C.
Gomes, A.P.S.
author2_role author
author
author
dc.contributor.author.fl_str_mv Araújo, J.V.
Junghans, T.G.
Alfenas, A.C.
Gomes, A.P.S.
dc.subject.pt-BR.fl_str_mv Nematophagous fungi
Nematode-trapping fungus
Predacious fungi
Arthrobotrys
Isoenzymes
Electrophoresis
topic Nematophagous fungi
Nematode-trapping fungus
Predacious fungi
Arthrobotrys
Isoenzymes
Electrophoresis
description Extraction and isoenzyme analysis of four isolates of rthrobotrys including A. musiformis, A. robusta and A. conoides were conducted. Among the 14 enzymes studied by starch gel electrophoresis, using morpholine-citrate as gel/electrode buffer, the following nine enzymes showed interpretable banding patterns: α-esterase, fumarase, hexokinase, isocitrate dehydrogenase, leucine aminopeptidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphogluco- mutase and phosphoglucoisomerase. All isolates studied displayed typical isoenzyme phenotypes for each species. Two isolates of A. conoides differed in their α-isoesterase banding patterns, but no differences were observed for the other enzymes. The assay was satisfactory for enzyme extraction and resolution of Arthrobotrys and could be used in future taxonomic and genetic studies of this organism.
publishDate 1997
dc.date.issued.fl_str_mv 1997-06-28
dc.date.accessioned.fl_str_mv 2017-10-09T18:32:34Z
dc.date.available.fl_str_mv 2017-10-09T18:32:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X1997001000001
http://www.locus.ufv.br/handle/123456789/11962
dc.identifier.issn.none.fl_str_mv 1414-431X
identifier_str_mv 1414-431X
url http://dx.doi.org/10.1590/S0100-879X1997001000001
http://www.locus.ufv.br/handle/123456789/11962
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv vol. 30 n. 10 1149-1152 Oct. 1997
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Brazilian Journal of Medical and Biological Research
publisher.none.fl_str_mv Brazilian Journal of Medical and Biological Research
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