Physiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stress
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.jtherbio.2006.08.009 http://www.locus.ufv.br/handle/123456789/22271 |
Resumo: | The trehalose cycle in filamentous fungi has been suggested to be an important mechanism of tolerance against adverse stress conditions, particularly in thermal stress. Here, we demonstrate that trehalose and trehalase activity can be involved as an additional mechanism in development of thermotolerance in Pisolithus sp. In response to heat shock at 42 °C, an accumulation of intracellular trehalose and an increase in trehalase activity in the mycelial mass of the fungus was observed, when compared to the control treatment (28 °C). In vitro, assays showed that trehalose had a protective effect on β-glucosidase activity under thermal stress. Therefore, trehalose production may be an important mechanism of protection in ectomycorrhizal fungi, and this capacity could be used in the selection of isolates with greater capacity for adaptation to environmental stress. |
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Tótola, Marcos R.Ferreira, Adão S.Borges, Arnaldo C.2018-10-16T11:36:12Z2018-10-16T11:36:12Z2007-0103064565https://doi.org/10.1016/j.jtherbio.2006.08.009http://www.locus.ufv.br/handle/123456789/22271The trehalose cycle in filamentous fungi has been suggested to be an important mechanism of tolerance against adverse stress conditions, particularly in thermal stress. Here, we demonstrate that trehalose and trehalase activity can be involved as an additional mechanism in development of thermotolerance in Pisolithus sp. In response to heat shock at 42 °C, an accumulation of intracellular trehalose and an increase in trehalase activity in the mycelial mass of the fungus was observed, when compared to the control treatment (28 °C). In vitro, assays showed that trehalose had a protective effect on β-glucosidase activity under thermal stress. Therefore, trehalose production may be an important mechanism of protection in ectomycorrhizal fungi, and this capacity could be used in the selection of isolates with greater capacity for adaptation to environmental stress.engJournal of Thermal Biologyv. 32, n.1, p. 34- 41, jan. 2007Elsevier Ltd.info:eu-repo/semantics/openAccessCellular protectionEctomycorrhizal fungusβ-GlucosidaseThermal stressTrehalasePhysiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stressinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf203036https://locus.ufv.br//bitstream/123456789/22271/1/artigo.pdffd514e9d44a1e9522abc0e3d461797bcMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22271/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/222712018-10-16 09:00:32.109oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-10-16T12:00:32LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Physiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stress |
title |
Physiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stress |
spellingShingle |
Physiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stress Tótola, Marcos R. Cellular protection Ectomycorrhizal fungus β-Glucosidase Thermal stress Trehalase |
title_short |
Physiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stress |
title_full |
Physiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stress |
title_fullStr |
Physiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stress |
title_full_unstemmed |
Physiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stress |
title_sort |
Physiological implications of trehalose in the ectomycorrhizal fungus Pisolithus sp. under thermal stress |
author |
Tótola, Marcos R. |
author_facet |
Tótola, Marcos R. Ferreira, Adão S. Borges, Arnaldo C. |
author_role |
author |
author2 |
Ferreira, Adão S. Borges, Arnaldo C. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Tótola, Marcos R. Ferreira, Adão S. Borges, Arnaldo C. |
dc.subject.pt-BR.fl_str_mv |
Cellular protection Ectomycorrhizal fungus β-Glucosidase Thermal stress Trehalase |
topic |
Cellular protection Ectomycorrhizal fungus β-Glucosidase Thermal stress Trehalase |
description |
The trehalose cycle in filamentous fungi has been suggested to be an important mechanism of tolerance against adverse stress conditions, particularly in thermal stress. Here, we demonstrate that trehalose and trehalase activity can be involved as an additional mechanism in development of thermotolerance in Pisolithus sp. In response to heat shock at 42 °C, an accumulation of intracellular trehalose and an increase in trehalase activity in the mycelial mass of the fungus was observed, when compared to the control treatment (28 °C). In vitro, assays showed that trehalose had a protective effect on β-glucosidase activity under thermal stress. Therefore, trehalose production may be an important mechanism of protection in ectomycorrhizal fungi, and this capacity could be used in the selection of isolates with greater capacity for adaptation to environmental stress. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-01 |
dc.date.accessioned.fl_str_mv |
2018-10-16T11:36:12Z |
dc.date.available.fl_str_mv |
2018-10-16T11:36:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.jtherbio.2006.08.009 http://www.locus.ufv.br/handle/123456789/22271 |
dc.identifier.issn.none.fl_str_mv |
03064565 |
identifier_str_mv |
03064565 |
url |
https://doi.org/10.1016/j.jtherbio.2006.08.009 http://www.locus.ufv.br/handle/123456789/22271 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 32, n.1, p. 34- 41, jan. 2007 |
dc.rights.driver.fl_str_mv |
Elsevier Ltd. info:eu-repo/semantics/openAccess |
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Elsevier Ltd. |
eu_rights_str_mv |
openAccess |
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Journal of Thermal Biology |
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Journal of Thermal Biology |
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LOCUS Repositório Institucional da UFV |
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