Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos

Detalhes bibliográficos
Autor(a) principal: Matta, Ana Paula de Lima Florentino
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/2307
Resumo: The utilization of plants with aphrodisiac action is a practice that has been increasing in the last years, a fact that awakes the interest of researchers from phytochemical, pharmacological and clinic investigations. Ouratea semiserrata is popularly used as astringent, anti- inflammatory and sexual stimulator. In this study, the goal was to evaluate the effects of aqueous extract from roots of O. semiserrata (AEROS) over the body and testicular biometry, seminiferous tubule and intertubule components of adult mice testes, also the extract being subjected to phytochemical prospection and the quantification of phenolics compounds. Twenty seven animals were used, divided into a control group (G1), which received distilled water, and two groups that received AEROS in the concentrations of 750 (G2) and 1500 (G3) mg/kg of body weight. The phytochemical prospection by thin layer chromatography revealed the presence of triterpenes/steroids, flavonoids, essential oils and tannins. The content of total polyphenols, proanthocyanidins, and flavonoids in the plant root were 3.57%, 2.07% e 0.11%, respectively. It is suggested that AEROS aphrodisiac potential is probably related to the presence of flavonoids and proanthocyanidins, compounds with vasodilatation activity. The treatment in both extract concentrations interfered in no significant way into body and testicular biometry and tubular morphometry, suggesting absence of alterations in the spermatogenic process and root toxicity. A reduction in volume proportion and connective tissue volume in both groups treated was present. The highest extract concentration promoted an increase in Leydig nuclear diameter and volume, and a decrease in macrophage proportion and blood vessels volume. Alterations in Leydig nuclear diameter and volume may be associated to increased levels of testosterone, probably, not enough to act over this testosterone-dependent reproductive organs weight.
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spelling Matta, Ana Paula de Lima Florentinohttp://lattes.cnpq.br/1893440680741142Leite, João Paulo Vianahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763897U8Paula, Tarcízio Antônio Rego dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701637D5Matta, Sérgio Luis Pinto dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798314Z0Neves, Clóvis Andradehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785611E1Melo, Fabiana Cristina Silveira Alves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794529J2Fonseca, Cláudio Césarhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780777E62015-03-26T13:04:06Z2011-05-232015-03-26T13:04:06Z2008-07-28MATTA, Ana Paula de Lima Florentino. Effects of aqueous extract from Ouratea semiserrata (Mart.) Engl. (Ochnaceae) root on Swiss adult mice testes. 2008. 66 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/2307The utilization of plants with aphrodisiac action is a practice that has been increasing in the last years, a fact that awakes the interest of researchers from phytochemical, pharmacological and clinic investigations. Ouratea semiserrata is popularly used as astringent, anti- inflammatory and sexual stimulator. In this study, the goal was to evaluate the effects of aqueous extract from roots of O. semiserrata (AEROS) over the body and testicular biometry, seminiferous tubule and intertubule components of adult mice testes, also the extract being subjected to phytochemical prospection and the quantification of phenolics compounds. Twenty seven animals were used, divided into a control group (G1), which received distilled water, and two groups that received AEROS in the concentrations of 750 (G2) and 1500 (G3) mg/kg of body weight. The phytochemical prospection by thin layer chromatography revealed the presence of triterpenes/steroids, flavonoids, essential oils and tannins. The content of total polyphenols, proanthocyanidins, and flavonoids in the plant root were 3.57%, 2.07% e 0.11%, respectively. It is suggested that AEROS aphrodisiac potential is probably related to the presence of flavonoids and proanthocyanidins, compounds with vasodilatation activity. The treatment in both extract concentrations interfered in no significant way into body and testicular biometry and tubular morphometry, suggesting absence of alterations in the spermatogenic process and root toxicity. A reduction in volume proportion and connective tissue volume in both groups treated was present. The highest extract concentration promoted an increase in Leydig nuclear diameter and volume, and a decrease in macrophage proportion and blood vessels volume. Alterations in Leydig nuclear diameter and volume may be associated to increased levels of testosterone, probably, not enough to act over this testosterone-dependent reproductive organs weight.A utilização de plantas com ação afrodisíaca é uma prática que tem aumentado nos últimos anos, o que desperta o interesse de pesquisadores em investigações fitoquímicas, farmacológicas e clínicas. Ouratea semiserrata é utilizada popularmente como adstringente, antiinflamatória e estimulante sexual. Neste trabalho, buscou-se avaliar os efeitos do extrato aquoso da raiz de O.semiserrata (EAROS) sobre a biometria corporal e testicular, túbulos seminíferos e elementos intertubulares dos testículos de camundongos adultos, sendo o extrato também submetido à prospecção fitoquímica e a quantificação de compostos fenólicos. Foram utilizados 27 animais, sendo um grupo controle (G1), que recebeu água destilada e dois que receberam o EAROS nas concentrações de 750 (G2) e 1500 (G3) mg/Kg de peso corporal. A prospecção fitoquímica por cromatografia em camada delgada revelou a presença de triterpenos/esteróides, flavonóides, óleos essenciais e taninos. Os teores de polifenóis totais, proantocianidinas e flavonóides na raiz da planta foram 3,57%; 2,07% e 0,11%, respectivamente. Sugere-se que o potencial afrodisíaco do EAROS, provavelmente esteja relacionado com a presença de flavonóides e proantocianidinas, compostos com atividade vasodilatadora. O tratamento em ambas as concentrações do extrato não interferiu de forma significativa na biometria corporal e testicular e na morfometria tubular, sugerindo ausência de alterações no processo espermatogênico e de toxicidade da raiz. Houve redução na proporção volumétrica e no volume de tecido conjuntivo em ambos os grupos tratados. A maior concentração do extrato promoveu aumento no diâmetro e volume nuclear de Leydig, e diminuição na proporção de macrófagos e no volume de vasos sanguíneos. As alterações no volume e diâmetro nuclear de Leydig podem estar associadas com aumento nos níveis de testosterona, provavelmente, não o suficiente para atuar sobre os pesos dos órgãos reprodutivos testosterona- dependentes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Biologia Celular e EstruturalUFVBRAnálises quantitativas e moleculares do Genoma; Biologia das células e dos tecidosReproduçãoMorfometria testicularRaiz de bugreCélula de LeydigFitoterapiaReproductionTesticular morphometryOuratea semiserrataLeydig cellPhytotherapyCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALEfeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultosEffects of aqueous extract from Ouratea semiserrata (Mart.) Engl. 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dc.title.por.fl_str_mv Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos
dc.title.alternative.eng.fl_str_mv Effects of aqueous extract from Ouratea semiserrata (Mart.) Engl. (Ochnaceae) root on Swiss adult mice testes
title Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos
spellingShingle Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos
Matta, Ana Paula de Lima Florentino
Reprodução
Morfometria testicular
Raiz de bugre
Célula de Leydig
Fitoterapia
Reproduction
Testicular morphometry
Ouratea semiserrata
Leydig cell
Phytotherapy
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos
title_full Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos
title_fullStr Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos
title_full_unstemmed Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos
title_sort Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos
author Matta, Ana Paula de Lima Florentino
author_facet Matta, Ana Paula de Lima Florentino
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/1893440680741142
dc.contributor.author.fl_str_mv Matta, Ana Paula de Lima Florentino
dc.contributor.advisor-co1.fl_str_mv Leite, João Paulo Viana
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763897U8
dc.contributor.advisor-co2.fl_str_mv Paula, Tarcízio Antônio Rego de
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701637D5
dc.contributor.advisor1.fl_str_mv Matta, Sérgio Luis Pinto da
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798314Z0
dc.contributor.referee1.fl_str_mv Neves, Clóvis Andrade
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785611E1
dc.contributor.referee2.fl_str_mv Melo, Fabiana Cristina Silveira Alves de
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794529J2
dc.contributor.referee3.fl_str_mv Fonseca, Cláudio César
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780777E6
contributor_str_mv Leite, João Paulo Viana
Paula, Tarcízio Antônio Rego de
Matta, Sérgio Luis Pinto da
Neves, Clóvis Andrade
Melo, Fabiana Cristina Silveira Alves de
Fonseca, Cláudio César
dc.subject.por.fl_str_mv Reprodução
Morfometria testicular
Raiz de bugre
Célula de Leydig
Fitoterapia
topic Reprodução
Morfometria testicular
Raiz de bugre
Célula de Leydig
Fitoterapia
Reproduction
Testicular morphometry
Ouratea semiserrata
Leydig cell
Phytotherapy
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.eng.fl_str_mv Reproduction
Testicular morphometry
Ouratea semiserrata
Leydig cell
Phytotherapy
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description The utilization of plants with aphrodisiac action is a practice that has been increasing in the last years, a fact that awakes the interest of researchers from phytochemical, pharmacological and clinic investigations. Ouratea semiserrata is popularly used as astringent, anti- inflammatory and sexual stimulator. In this study, the goal was to evaluate the effects of aqueous extract from roots of O. semiserrata (AEROS) over the body and testicular biometry, seminiferous tubule and intertubule components of adult mice testes, also the extract being subjected to phytochemical prospection and the quantification of phenolics compounds. Twenty seven animals were used, divided into a control group (G1), which received distilled water, and two groups that received AEROS in the concentrations of 750 (G2) and 1500 (G3) mg/kg of body weight. The phytochemical prospection by thin layer chromatography revealed the presence of triterpenes/steroids, flavonoids, essential oils and tannins. The content of total polyphenols, proanthocyanidins, and flavonoids in the plant root were 3.57%, 2.07% e 0.11%, respectively. It is suggested that AEROS aphrodisiac potential is probably related to the presence of flavonoids and proanthocyanidins, compounds with vasodilatation activity. The treatment in both extract concentrations interfered in no significant way into body and testicular biometry and tubular morphometry, suggesting absence of alterations in the spermatogenic process and root toxicity. A reduction in volume proportion and connective tissue volume in both groups treated was present. The highest extract concentration promoted an increase in Leydig nuclear diameter and volume, and a decrease in macrophage proportion and blood vessels volume. Alterations in Leydig nuclear diameter and volume may be associated to increased levels of testosterone, probably, not enough to act over this testosterone-dependent reproductive organs weight.
publishDate 2008
dc.date.issued.fl_str_mv 2008-07-28
dc.date.available.fl_str_mv 2011-05-23
2015-03-26T13:04:06Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:04:06Z
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dc.identifier.citation.fl_str_mv MATTA, Ana Paula de Lima Florentino. Effects of aqueous extract from Ouratea semiserrata (Mart.) Engl. (Ochnaceae) root on Swiss adult mice testes. 2008. 66 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2008.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/2307
identifier_str_mv MATTA, Ana Paula de Lima Florentino. Effects of aqueous extract from Ouratea semiserrata (Mart.) Engl. (Ochnaceae) root on Swiss adult mice testes. 2008. 66 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2008.
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