Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/2307 |
Resumo: | The utilization of plants with aphrodisiac action is a practice that has been increasing in the last years, a fact that awakes the interest of researchers from phytochemical, pharmacological and clinic investigations. Ouratea semiserrata is popularly used as astringent, anti- inflammatory and sexual stimulator. In this study, the goal was to evaluate the effects of aqueous extract from roots of O. semiserrata (AEROS) over the body and testicular biometry, seminiferous tubule and intertubule components of adult mice testes, also the extract being subjected to phytochemical prospection and the quantification of phenolics compounds. Twenty seven animals were used, divided into a control group (G1), which received distilled water, and two groups that received AEROS in the concentrations of 750 (G2) and 1500 (G3) mg/kg of body weight. The phytochemical prospection by thin layer chromatography revealed the presence of triterpenes/steroids, flavonoids, essential oils and tannins. The content of total polyphenols, proanthocyanidins, and flavonoids in the plant root were 3.57%, 2.07% e 0.11%, respectively. It is suggested that AEROS aphrodisiac potential is probably related to the presence of flavonoids and proanthocyanidins, compounds with vasodilatation activity. The treatment in both extract concentrations interfered in no significant way into body and testicular biometry and tubular morphometry, suggesting absence of alterations in the spermatogenic process and root toxicity. A reduction in volume proportion and connective tissue volume in both groups treated was present. The highest extract concentration promoted an increase in Leydig nuclear diameter and volume, and a decrease in macrophage proportion and blood vessels volume. Alterations in Leydig nuclear diameter and volume may be associated to increased levels of testosterone, probably, not enough to act over this testosterone-dependent reproductive organs weight. |
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Matta, Ana Paula de Lima Florentinohttp://lattes.cnpq.br/1893440680741142Leite, João Paulo Vianahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763897U8Paula, Tarcízio Antônio Rego dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701637D5Matta, Sérgio Luis Pinto dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798314Z0Neves, Clóvis Andradehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785611E1Melo, Fabiana Cristina Silveira Alves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794529J2Fonseca, Cláudio Césarhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780777E62015-03-26T13:04:06Z2011-05-232015-03-26T13:04:06Z2008-07-28MATTA, Ana Paula de Lima Florentino. Effects of aqueous extract from Ouratea semiserrata (Mart.) Engl. (Ochnaceae) root on Swiss adult mice testes. 2008. 66 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/2307The utilization of plants with aphrodisiac action is a practice that has been increasing in the last years, a fact that awakes the interest of researchers from phytochemical, pharmacological and clinic investigations. Ouratea semiserrata is popularly used as astringent, anti- inflammatory and sexual stimulator. In this study, the goal was to evaluate the effects of aqueous extract from roots of O. semiserrata (AEROS) over the body and testicular biometry, seminiferous tubule and intertubule components of adult mice testes, also the extract being subjected to phytochemical prospection and the quantification of phenolics compounds. Twenty seven animals were used, divided into a control group (G1), which received distilled water, and two groups that received AEROS in the concentrations of 750 (G2) and 1500 (G3) mg/kg of body weight. The phytochemical prospection by thin layer chromatography revealed the presence of triterpenes/steroids, flavonoids, essential oils and tannins. The content of total polyphenols, proanthocyanidins, and flavonoids in the plant root were 3.57%, 2.07% e 0.11%, respectively. It is suggested that AEROS aphrodisiac potential is probably related to the presence of flavonoids and proanthocyanidins, compounds with vasodilatation activity. The treatment in both extract concentrations interfered in no significant way into body and testicular biometry and tubular morphometry, suggesting absence of alterations in the spermatogenic process and root toxicity. A reduction in volume proportion and connective tissue volume in both groups treated was present. The highest extract concentration promoted an increase in Leydig nuclear diameter and volume, and a decrease in macrophage proportion and blood vessels volume. Alterations in Leydig nuclear diameter and volume may be associated to increased levels of testosterone, probably, not enough to act over this testosterone-dependent reproductive organs weight.A utilização de plantas com ação afrodisíaca é uma prática que tem aumentado nos últimos anos, o que desperta o interesse de pesquisadores em investigações fitoquímicas, farmacológicas e clínicas. Ouratea semiserrata é utilizada popularmente como adstringente, antiinflamatória e estimulante sexual. Neste trabalho, buscou-se avaliar os efeitos do extrato aquoso da raiz de O.semiserrata (EAROS) sobre a biometria corporal e testicular, túbulos seminíferos e elementos intertubulares dos testículos de camundongos adultos, sendo o extrato também submetido à prospecção fitoquímica e a quantificação de compostos fenólicos. Foram utilizados 27 animais, sendo um grupo controle (G1), que recebeu água destilada e dois que receberam o EAROS nas concentrações de 750 (G2) e 1500 (G3) mg/Kg de peso corporal. A prospecção fitoquímica por cromatografia em camada delgada revelou a presença de triterpenos/esteróides, flavonóides, óleos essenciais e taninos. Os teores de polifenóis totais, proantocianidinas e flavonóides na raiz da planta foram 3,57%; 2,07% e 0,11%, respectivamente. Sugere-se que o potencial afrodisíaco do EAROS, provavelmente esteja relacionado com a presença de flavonóides e proantocianidinas, compostos com atividade vasodilatadora. O tratamento em ambas as concentrações do extrato não interferiu de forma significativa na biometria corporal e testicular e na morfometria tubular, sugerindo ausência de alterações no processo espermatogênico e de toxicidade da raiz. Houve redução na proporção volumétrica e no volume de tecido conjuntivo em ambos os grupos tratados. A maior concentração do extrato promoveu aumento no diâmetro e volume nuclear de Leydig, e diminuição na proporção de macrófagos e no volume de vasos sanguíneos. As alterações no volume e diâmetro nuclear de Leydig podem estar associadas com aumento nos níveis de testosterona, provavelmente, não o suficiente para atuar sobre os pesos dos órgãos reprodutivos testosterona- dependentes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Biologia Celular e EstruturalUFVBRAnálises quantitativas e moleculares do Genoma; Biologia das células e dos tecidosReproduçãoMorfometria testicularRaiz de bugreCélula de LeydigFitoterapiaReproductionTesticular morphometryOuratea semiserrataLeydig cellPhytotherapyCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALEfeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultosEffects of aqueous extract from Ouratea semiserrata (Mart.) Engl. (Ochnaceae) root on Swiss adult mice testesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf310832https://locus.ufv.br//bitstream/123456789/2307/1/texto%20completo.pdf46204e20e1493d119ca08ec64b548f99MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain139855https://locus.ufv.br//bitstream/123456789/2307/2/texto%20completo.pdf.txtfd8076a2489cef8d307e5f95182013a9MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3526https://locus.ufv.br//bitstream/123456789/2307/3/texto%20completo.pdf.jpg274c796f020a5f89b15fb9247efb1735MD53123456789/23072016-04-08 23:05:25.394oai:locus.ufv.br:123456789/2307Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-09T02:05:25LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos |
dc.title.alternative.eng.fl_str_mv |
Effects of aqueous extract from Ouratea semiserrata (Mart.) Engl. (Ochnaceae) root on Swiss adult mice testes |
title |
Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos |
spellingShingle |
Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos Matta, Ana Paula de Lima Florentino Reprodução Morfometria testicular Raiz de bugre Célula de Leydig Fitoterapia Reproduction Testicular morphometry Ouratea semiserrata Leydig cell Phytotherapy CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos |
title_full |
Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos |
title_fullStr |
Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos |
title_full_unstemmed |
Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos |
title_sort |
Efeitos do extrato aquoso da raiz de Ouratea semiserrata (Mart.) Engl. (Ochnaceae) sobre os testículos de camundongos suíços adultos |
author |
Matta, Ana Paula de Lima Florentino |
author_facet |
Matta, Ana Paula de Lima Florentino |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/1893440680741142 |
dc.contributor.author.fl_str_mv |
Matta, Ana Paula de Lima Florentino |
dc.contributor.advisor-co1.fl_str_mv |
Leite, João Paulo Viana |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763897U8 |
dc.contributor.advisor-co2.fl_str_mv |
Paula, Tarcízio Antônio Rego de |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701637D5 |
dc.contributor.advisor1.fl_str_mv |
Matta, Sérgio Luis Pinto da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798314Z0 |
dc.contributor.referee1.fl_str_mv |
Neves, Clóvis Andrade |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785611E1 |
dc.contributor.referee2.fl_str_mv |
Melo, Fabiana Cristina Silveira Alves de |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794529J2 |
dc.contributor.referee3.fl_str_mv |
Fonseca, Cláudio César |
dc.contributor.referee3Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780777E6 |
contributor_str_mv |
Leite, João Paulo Viana Paula, Tarcízio Antônio Rego de Matta, Sérgio Luis Pinto da Neves, Clóvis Andrade Melo, Fabiana Cristina Silveira Alves de Fonseca, Cláudio César |
dc.subject.por.fl_str_mv |
Reprodução Morfometria testicular Raiz de bugre Célula de Leydig Fitoterapia |
topic |
Reprodução Morfometria testicular Raiz de bugre Célula de Leydig Fitoterapia Reproduction Testicular morphometry Ouratea semiserrata Leydig cell Phytotherapy CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Reproduction Testicular morphometry Ouratea semiserrata Leydig cell Phytotherapy |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
The utilization of plants with aphrodisiac action is a practice that has been increasing in the last years, a fact that awakes the interest of researchers from phytochemical, pharmacological and clinic investigations. Ouratea semiserrata is popularly used as astringent, anti- inflammatory and sexual stimulator. In this study, the goal was to evaluate the effects of aqueous extract from roots of O. semiserrata (AEROS) over the body and testicular biometry, seminiferous tubule and intertubule components of adult mice testes, also the extract being subjected to phytochemical prospection and the quantification of phenolics compounds. Twenty seven animals were used, divided into a control group (G1), which received distilled water, and two groups that received AEROS in the concentrations of 750 (G2) and 1500 (G3) mg/kg of body weight. The phytochemical prospection by thin layer chromatography revealed the presence of triterpenes/steroids, flavonoids, essential oils and tannins. The content of total polyphenols, proanthocyanidins, and flavonoids in the plant root were 3.57%, 2.07% e 0.11%, respectively. It is suggested that AEROS aphrodisiac potential is probably related to the presence of flavonoids and proanthocyanidins, compounds with vasodilatation activity. The treatment in both extract concentrations interfered in no significant way into body and testicular biometry and tubular morphometry, suggesting absence of alterations in the spermatogenic process and root toxicity. A reduction in volume proportion and connective tissue volume in both groups treated was present. The highest extract concentration promoted an increase in Leydig nuclear diameter and volume, and a decrease in macrophage proportion and blood vessels volume. Alterations in Leydig nuclear diameter and volume may be associated to increased levels of testosterone, probably, not enough to act over this testosterone-dependent reproductive organs weight. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-07-28 |
dc.date.available.fl_str_mv |
2011-05-23 2015-03-26T13:04:06Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:04:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
MATTA, Ana Paula de Lima Florentino. Effects of aqueous extract from Ouratea semiserrata (Mart.) Engl. (Ochnaceae) root on Swiss adult mice testes. 2008. 66 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2008. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/2307 |
identifier_str_mv |
MATTA, Ana Paula de Lima Florentino. Effects of aqueous extract from Ouratea semiserrata (Mart.) Engl. (Ochnaceae) root on Swiss adult mice testes. 2008. 66 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2008. |
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http://locus.ufv.br/handle/123456789/2307 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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Universidade Federal de Viçosa |
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Mestrado em Biologia Celular e Estrutural |
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UFV |
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BR |
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Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos |
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Universidade Federal de Viçosa |
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