Wounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet root
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.postharvbio.2005.10.019 http://www.locus.ufv.br/handle/123456789/22548 |
Resumo: | The wounding of sugarbeet (Beta vulgaris L.) roots by harvesting and piling operations increases the demand for sucrolytic and glycolytic products for wound-healing processes. To determine if sucrolytic and glycolytic enzyme activities increase to meet this demand and to identify those activities that may be induced, the activities of the major sucrolytic enzymes and the major regulatory enzymes of the glycolytic pathway were determined in wounded and unwounded sugarbeet roots during 13 days of storage at 10 °C. Activities of the enzymes responsible for catalysis of the first two reactions of the glycolytic pathway, hexokinase, fructokinase and phosphofructokinase, were elevated in wounded roots. Activities of the sucrolytic enzymes, sucrose synthase, alkaline invertase, and soluble acid invertase, and the glycolytic enzyme, pyruvate kinase, did not increase in wounded roots. The activities of the early glycolytic enzymes peaked 24–48 h after wounding when the demand for substrates for wound-healing processes was expected to be maximal. Fructokinase exhibited the greatest and most persistent increase in activity, increasing by 150%, 24 h after wounding and remaining elevated for the duration of the study. The increase in hexokinase, fructokinase, and phosphofructokinase activities suggests that expression of these early glycolytic enzymes may be up-regulated to meet the demand for glycolytic intermediates and products for wound-healing processes. The lack of an increase in any sucrolytic activities in response to wounding suggests that sucrolytic flux is not determined by the quantity of active protein present in the root, but by some undetermined mechanism. |
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Finge, Fernando L.Klotz, Karen L.Anderson, Marc D.2018-11-13T19:10:34Z2018-11-13T19:10:34Z2006-0709255214https://doi.org/10.1016/j.postharvbio.2005.10.019http://www.locus.ufv.br/handle/123456789/22548The wounding of sugarbeet (Beta vulgaris L.) roots by harvesting and piling operations increases the demand for sucrolytic and glycolytic products for wound-healing processes. To determine if sucrolytic and glycolytic enzyme activities increase to meet this demand and to identify those activities that may be induced, the activities of the major sucrolytic enzymes and the major regulatory enzymes of the glycolytic pathway were determined in wounded and unwounded sugarbeet roots during 13 days of storage at 10 °C. Activities of the enzymes responsible for catalysis of the first two reactions of the glycolytic pathway, hexokinase, fructokinase and phosphofructokinase, were elevated in wounded roots. Activities of the sucrolytic enzymes, sucrose synthase, alkaline invertase, and soluble acid invertase, and the glycolytic enzyme, pyruvate kinase, did not increase in wounded roots. The activities of the early glycolytic enzymes peaked 24–48 h after wounding when the demand for substrates for wound-healing processes was expected to be maximal. Fructokinase exhibited the greatest and most persistent increase in activity, increasing by 150%, 24 h after wounding and remaining elevated for the duration of the study. The increase in hexokinase, fructokinase, and phosphofructokinase activities suggests that expression of these early glycolytic enzymes may be up-regulated to meet the demand for glycolytic intermediates and products for wound-healing processes. The lack of an increase in any sucrolytic activities in response to wounding suggests that sucrolytic flux is not determined by the quantity of active protein present in the root, but by some undetermined mechanism.engPostharvest Biology and TechnologyVolume 41, Issue 1, Pages 48- 55, July 20062006 Elsevier B.V. All rights reserved.info:eu-repo/semantics/openAccessAcid invertaseAlkaline invertaseBeta vulgarisFructokinaseHexokinasePhosphofructokinasePyruvate kinaseSucrose synthaseWoundingWounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet rootinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf388423https://locus.ufv.br//bitstream/123456789/22548/1/artigo.pdff9e0cf71c6816da213744a2d416d0155MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/22548/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/225482018-11-13 16:47:20.77oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-11-13T19:47:20LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Wounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet root |
title |
Wounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet root |
spellingShingle |
Wounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet root Finge, Fernando L. Acid invertase Alkaline invertase Beta vulgaris Fructokinase Hexokinase Phosphofructokinase Pyruvate kinase Sucrose synthase Wounding |
title_short |
Wounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet root |
title_full |
Wounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet root |
title_fullStr |
Wounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet root |
title_full_unstemmed |
Wounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet root |
title_sort |
Wounding increases glycolytic but not soluble sucrolytic activities in stored sugarbeet root |
author |
Finge, Fernando L. |
author_facet |
Finge, Fernando L. Klotz, Karen L. Anderson, Marc D. |
author_role |
author |
author2 |
Klotz, Karen L. Anderson, Marc D. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Finge, Fernando L. Klotz, Karen L. Anderson, Marc D. |
dc.subject.pt-BR.fl_str_mv |
Acid invertase Alkaline invertase Beta vulgaris Fructokinase Hexokinase Phosphofructokinase Pyruvate kinase Sucrose synthase Wounding |
topic |
Acid invertase Alkaline invertase Beta vulgaris Fructokinase Hexokinase Phosphofructokinase Pyruvate kinase Sucrose synthase Wounding |
description |
The wounding of sugarbeet (Beta vulgaris L.) roots by harvesting and piling operations increases the demand for sucrolytic and glycolytic products for wound-healing processes. To determine if sucrolytic and glycolytic enzyme activities increase to meet this demand and to identify those activities that may be induced, the activities of the major sucrolytic enzymes and the major regulatory enzymes of the glycolytic pathway were determined in wounded and unwounded sugarbeet roots during 13 days of storage at 10 °C. Activities of the enzymes responsible for catalysis of the first two reactions of the glycolytic pathway, hexokinase, fructokinase and phosphofructokinase, were elevated in wounded roots. Activities of the sucrolytic enzymes, sucrose synthase, alkaline invertase, and soluble acid invertase, and the glycolytic enzyme, pyruvate kinase, did not increase in wounded roots. The activities of the early glycolytic enzymes peaked 24–48 h after wounding when the demand for substrates for wound-healing processes was expected to be maximal. Fructokinase exhibited the greatest and most persistent increase in activity, increasing by 150%, 24 h after wounding and remaining elevated for the duration of the study. The increase in hexokinase, fructokinase, and phosphofructokinase activities suggests that expression of these early glycolytic enzymes may be up-regulated to meet the demand for glycolytic intermediates and products for wound-healing processes. The lack of an increase in any sucrolytic activities in response to wounding suggests that sucrolytic flux is not determined by the quantity of active protein present in the root, but by some undetermined mechanism. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-07 |
dc.date.accessioned.fl_str_mv |
2018-11-13T19:10:34Z |
dc.date.available.fl_str_mv |
2018-11-13T19:10:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.postharvbio.2005.10.019 http://www.locus.ufv.br/handle/123456789/22548 |
dc.identifier.issn.none.fl_str_mv |
09255214 |
identifier_str_mv |
09255214 |
url |
https://doi.org/10.1016/j.postharvbio.2005.10.019 http://www.locus.ufv.br/handle/123456789/22548 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volume 41, Issue 1, Pages 48- 55, July 2006 |
dc.rights.driver.fl_str_mv |
2006 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
2006 Elsevier B.V. All rights reserved. |
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openAccess |
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Postharvest Biology and Technology |
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Postharvest Biology and Technology |
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