Estrutura e diversidade genética no complexo Cedrela fissilis (Meliaceae) estimadas com marcadores microssatélites

• Autor(a) principal: Mangaravite, érica
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4774
• topic: Cedrela fissilis; Marcadores microssatélites; Diversidade genética; Cedrela fissilis; Microsatellite markers; Genetic diversity; CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL

The knowledge of how genetic variation is partitioned among populations may have important implications in evolutionary, ecology and conservation biology. The Cedrela fissilis species (Meliaceae) is a tree associated with Seasonal and Moist Forest. In addition, this species is considered "endangered" on the red list of the IUCN due to the loss of habitat and its high timber value. C. fissilis is formed by two phylogenetic lineages that are not a monophyletic clade, comprising sequences of C. odorata and C. balansae, called C. fissilis complex. The aim of this study was to characterize the genetic diversity of C. fissilis complex intra and inter populations in Brazil and Bolivia. Eight microsatellites were used to genotype 245 individuals, then it was obtained high values of heterozygosity (HO=0.73, HE=0.81), with positive values of FIS (FIS=0.11), indicating excess of homozygotes. For AMOVA, 94.39% of the variation were within populations and 5.61% were among populations which also reflected low levels of genetic divergence (FST=0.075). The populations of the Atlantic range showed slightly more diverse, with 20 of 37 private alleles found. It presented FST value (FST=0.063) greater than the populations of Amazonic-Chiquitano range (FST=0.022). It has been found weak populations structure showing a number of groups equal to eight and being greater than the number of known phylogenetic lineages based on DNA sequences. The low interpopulation differences found suggest the same mechanisms for conservation management. Furthermore, some populations showed conservation relevance due to the presence of peculiar genetic material and characteristics of refuge.