Kinetics of growth and ethanol formation from a mix of glucose/xylose substrate by Kluyveromyces marxianus UFV-3
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1007/s10482-012-9794-z http://www.locus.ufv.br/handle/123456789/21822 |
Resumo: | The fermentation of both glucose and xylose is important to maximize ethanol yield from renewable biomass feedstocks. In this article, we analyze growth, sugar consumption, and ethanol formation by the yeast Kluyveromyces marxianus UFV-3 using various glucose and xylose concentrations and also under conditions of reduced respiratory activity. In almost all the conditions analyzed, glucose repressed xylose assimilation and xylose consumption began after glucose had been exhausted. A remarkable difference was observed when mixtures of 5 g L^−1 glucose/20 g L^−1 xylose and 20 g L^−1 glucose/20 g L^−1 xylose were used. In the former, the xylose consumption began immediately after the glucose depletion. Indeed, there was no striking diauxic phase, as observed in the latter condition, in which there was an interval of 30 h between glucose depletion and the beginning of xylose consumption. Ethanol production was always higher in a mixture of glucose and xylose than in glucose alone. The highest ethanol concentration (8.65 g L^−1) and cell mass concentration (4.42 g L^−1) were achieved after 8 and 74 h, respectively, in a mixture of 20 g L^−1 glucose/20 g L^−1 xylose. When inhibitors of respiration were added to the medium, glucose repression of xylose consumption was alleviated completely and K. marxianus was able to consume xylose and glucose simultaneously. |
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LOCUS Repositório Institucional da UFV |
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2145 |
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Kinetics of growth and ethanol formation from a mix of glucose/xylose substrate by Kluyveromyces marxianus UFV-3FermentationGlucoseXyloseKluyveromyces marxianus UFV-3BioethanolThe fermentation of both glucose and xylose is important to maximize ethanol yield from renewable biomass feedstocks. In this article, we analyze growth, sugar consumption, and ethanol formation by the yeast Kluyveromyces marxianus UFV-3 using various glucose and xylose concentrations and also under conditions of reduced respiratory activity. In almost all the conditions analyzed, glucose repressed xylose assimilation and xylose consumption began after glucose had been exhausted. A remarkable difference was observed when mixtures of 5 g L^−1 glucose/20 g L^−1 xylose and 20 g L^−1 glucose/20 g L^−1 xylose were used. In the former, the xylose consumption began immediately after the glucose depletion. Indeed, there was no striking diauxic phase, as observed in the latter condition, in which there was an interval of 30 h between glucose depletion and the beginning of xylose consumption. Ethanol production was always higher in a mixture of glucose and xylose than in glucose alone. The highest ethanol concentration (8.65 g L^−1) and cell mass concentration (4.42 g L^−1) were achieved after 8 and 74 h, respectively, in a mixture of 20 g L^−1 glucose/20 g L^−1 xylose. When inhibitors of respiration were added to the medium, glucose repression of xylose consumption was alleviated completely and K. marxianus was able to consume xylose and glucose simultaneously.Antonie van Leeuwenhoek2018-09-13T18:27:09Z2018-09-13T18:27:09Z2012-09-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf15729699http://dx.doi.org/10.1007/s10482-012-9794-zhttp://www.locus.ufv.br/handle/123456789/21822engv. 103, n. 1, p. 153– 161, jan. 2013Springer Nature Switzerland AG.info:eu-repo/semantics/openAccessPassos, Flávia Maria LopesSantos, Valdilene Canazart dosBragança, Caio Roberto SoaresPassos, Frederico José Vieirareponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T08:45:02Zoai:locus.ufv.br:123456789/21822Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T08:45:02LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Kinetics of growth and ethanol formation from a mix of glucose/xylose substrate by Kluyveromyces marxianus UFV-3 |
title |
Kinetics of growth and ethanol formation from a mix of glucose/xylose substrate by Kluyveromyces marxianus UFV-3 |
spellingShingle |
Kinetics of growth and ethanol formation from a mix of glucose/xylose substrate by Kluyveromyces marxianus UFV-3 Passos, Flávia Maria Lopes Fermentation Glucose Xylose Kluyveromyces marxianus UFV-3 Bioethanol |
title_short |
Kinetics of growth and ethanol formation from a mix of glucose/xylose substrate by Kluyveromyces marxianus UFV-3 |
title_full |
Kinetics of growth and ethanol formation from a mix of glucose/xylose substrate by Kluyveromyces marxianus UFV-3 |
title_fullStr |
Kinetics of growth and ethanol formation from a mix of glucose/xylose substrate by Kluyveromyces marxianus UFV-3 |
title_full_unstemmed |
Kinetics of growth and ethanol formation from a mix of glucose/xylose substrate by Kluyveromyces marxianus UFV-3 |
title_sort |
Kinetics of growth and ethanol formation from a mix of glucose/xylose substrate by Kluyveromyces marxianus UFV-3 |
author |
Passos, Flávia Maria Lopes |
author_facet |
Passos, Flávia Maria Lopes Santos, Valdilene Canazart dos Bragança, Caio Roberto Soares Passos, Frederico José Vieira |
author_role |
author |
author2 |
Santos, Valdilene Canazart dos Bragança, Caio Roberto Soares Passos, Frederico José Vieira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Passos, Flávia Maria Lopes Santos, Valdilene Canazart dos Bragança, Caio Roberto Soares Passos, Frederico José Vieira |
dc.subject.por.fl_str_mv |
Fermentation Glucose Xylose Kluyveromyces marxianus UFV-3 Bioethanol |
topic |
Fermentation Glucose Xylose Kluyveromyces marxianus UFV-3 Bioethanol |
description |
The fermentation of both glucose and xylose is important to maximize ethanol yield from renewable biomass feedstocks. In this article, we analyze growth, sugar consumption, and ethanol formation by the yeast Kluyveromyces marxianus UFV-3 using various glucose and xylose concentrations and also under conditions of reduced respiratory activity. In almost all the conditions analyzed, glucose repressed xylose assimilation and xylose consumption began after glucose had been exhausted. A remarkable difference was observed when mixtures of 5 g L^−1 glucose/20 g L^−1 xylose and 20 g L^−1 glucose/20 g L^−1 xylose were used. In the former, the xylose consumption began immediately after the glucose depletion. Indeed, there was no striking diauxic phase, as observed in the latter condition, in which there was an interval of 30 h between glucose depletion and the beginning of xylose consumption. Ethanol production was always higher in a mixture of glucose and xylose than in glucose alone. The highest ethanol concentration (8.65 g L^−1) and cell mass concentration (4.42 g L^−1) were achieved after 8 and 74 h, respectively, in a mixture of 20 g L^−1 glucose/20 g L^−1 xylose. When inhibitors of respiration were added to the medium, glucose repression of xylose consumption was alleviated completely and K. marxianus was able to consume xylose and glucose simultaneously. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-11 2018-09-13T18:27:09Z 2018-09-13T18:27:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
15729699 http://dx.doi.org/10.1007/s10482-012-9794-z http://www.locus.ufv.br/handle/123456789/21822 |
identifier_str_mv |
15729699 |
url |
http://dx.doi.org/10.1007/s10482-012-9794-z http://www.locus.ufv.br/handle/123456789/21822 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
v. 103, n. 1, p. 153– 161, jan. 2013 |
dc.rights.driver.fl_str_mv |
Springer Nature Switzerland AG. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Springer Nature Switzerland AG. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Antonie van Leeuwenhoek |
publisher.none.fl_str_mv |
Antonie van Leeuwenhoek |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1817560040342552576 |