Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae

Detalhes bibliográficos
Autor(a) principal: Araujo, Marcela Uli Peixoto
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://locus.ufv.br//handle/123456789/27457
Resumo: Blast, caused by Pyricularia oryzae, has become an economically important disease on wheat in Brazil. Alternative methods for blast management are demanded by the growers and silicon (Si) stands out for its potential to decrease the intensities of important diseases in several crops. The present study aimed to investigate the effect of Si in improving the production of photoassimilates on flag leaves and their partitioning to spikes in a scenario where the blast symptoms will be lowered due to the potentiation of mechanisms of defense by this element on the source-sink interface. Wheat plants (cultivar BRS-Guamirim) were grown in hydroponic culture with either 0 or 2 mM Si and plants were inoculated with P. oryzae at 10 days after anthesis. The Si concentration on flag leaves and spikes of plants supplied with Si increased and contributed to decrease disease symptoms. Higher concentrations of total soluble phenolics and lignin-thioglycolic acid derivatives and greater activities of peroxidases, polyphenoloxidases, phenylalanine ammonia-lyases, β-1,3- glucanases and chitinases were noticed on flag leaves and spikes of plants supplied with Si and contributed to their resistance to blast. There was less concentration of pigments and an impairment of the photosynthetic performance of infected flag leaves and spikes from plants non-supplied with Si based on the values for the chlorophyll a fluorescence parameters maximal photosystem II quantum efficiency, fraction of energy absorbed used in photochemistry, quantum yield of non-regulated energy dissipation and quantum yield of regulated energy dissipation. On infected flag leaves and spikes from plants non-supplied with Si, the concentration of soluble sugars was lower while the hexoses-to-sucrose ratio increased on infected flag leaves. The sucrose phosphate synthase activity was lower while higher expression and activity of acid invertases was higher and lower, respectively, on flag leaves and spikes of plants non-supplied with Si in comparison to Si-supplied plants. The starch concentration on spikes of plants supplied with Si increased. All together, the results from the present study confirm the active role played by Si in increasing wheat resistance to blast, but present novel evidences of the effect of this element in improve the source-sink relationship on infected flag leaves and spikes by preserving the alterations in assimilate production and partitioning during the grain filling process.
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spelling Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzaeSilício alivia as mudanças na relação fonte-dreno em plantas de trigo infectadas por Pyricularia oryzaeBrusonePyricularia oryzaeTriticum aestivumFotossínteseFitopatologiaBlast, caused by Pyricularia oryzae, has become an economically important disease on wheat in Brazil. Alternative methods for blast management are demanded by the growers and silicon (Si) stands out for its potential to decrease the intensities of important diseases in several crops. The present study aimed to investigate the effect of Si in improving the production of photoassimilates on flag leaves and their partitioning to spikes in a scenario where the blast symptoms will be lowered due to the potentiation of mechanisms of defense by this element on the source-sink interface. Wheat plants (cultivar BRS-Guamirim) were grown in hydroponic culture with either 0 or 2 mM Si and plants were inoculated with P. oryzae at 10 days after anthesis. The Si concentration on flag leaves and spikes of plants supplied with Si increased and contributed to decrease disease symptoms. Higher concentrations of total soluble phenolics and lignin-thioglycolic acid derivatives and greater activities of peroxidases, polyphenoloxidases, phenylalanine ammonia-lyases, β-1,3- glucanases and chitinases were noticed on flag leaves and spikes of plants supplied with Si and contributed to their resistance to blast. There was less concentration of pigments and an impairment of the photosynthetic performance of infected flag leaves and spikes from plants non-supplied with Si based on the values for the chlorophyll a fluorescence parameters maximal photosystem II quantum efficiency, fraction of energy absorbed used in photochemistry, quantum yield of non-regulated energy dissipation and quantum yield of regulated energy dissipation. On infected flag leaves and spikes from plants non-supplied with Si, the concentration of soluble sugars was lower while the hexoses-to-sucrose ratio increased on infected flag leaves. The sucrose phosphate synthase activity was lower while higher expression and activity of acid invertases was higher and lower, respectively, on flag leaves and spikes of plants non-supplied with Si in comparison to Si-supplied plants. The starch concentration on spikes of plants supplied with Si increased. All together, the results from the present study confirm the active role played by Si in increasing wheat resistance to blast, but present novel evidences of the effect of this element in improve the source-sink relationship on infected flag leaves and spikes by preserving the alterations in assimilate production and partitioning during the grain filling process.A brusone, causada por Pyricularia oryzae, tornou-se uma doença no trigo economicamente importante no Brasil. Métodos alternativos para o manejo da brusone são exigidos pelos produtores e o silício (Si) se destaca por seu potencial para diminuir a intensidade de doenças importantes em várias culturas. O presente estudo teve como objetivo investigar o efeito de Si em melhorar a produção de fotoassimilados em folhas bandeiras e espigas em um cenário onde os sintomas da brusone diminuem devido à potencialização de mecanismos de defesa por este elemento na relação fonte-dreno. As plantas de trigo (cultivar BRS-Guamirim) foram cultivadas em cultura hidropônica com 0 ou 2 mM de Si e as plantas foram inoculadas com P. oryzae aos 10 dias após a antese. A concentração de Si em folhas bandeiras e espigas de plantas fornecidas com Si aumentou e contribuiu para diminuir os sintomas da doença. As concentrações mais elevadas de fenóis solúveis totais e derivados de ácido lignina-tioglicólico e maiores atividades de peroxidases, polifenoloxidases, fenilalanina amônia-liases, β-1,3-glucanases e quitinases foram notadas em folhas bandeiras e espigas de plantas fornecidas com Si e contribuíram para a sua resistência à brusone. Houve menos concentração de pigmentos e um comprometimento da performance fotossintética de folhas bandeiras e espigas infectadas de plantas não fornecidas com Si com base nos valores dos parâmetros da fluorescência da clorofila a a partir da máxima eficiência quântica do fotossistema II, fração de energia absorvida usada em fotoquímica, rendimento quântico de dissipação de energia não regulada e produção quântica de dissipação de energia regulada. Nas folhas bandeiras e espigas infectadas de plantas não fornecidas com Si, a concentração de açúcares solúveis foi menor, enquanto a relação hexose-sacarose aumentou em folhas bandeiras infectadas. A atividade da sacarose fosfato sintase foi menor, enquanto a maior expressão e atividade das invertases ácidas foi maior e menor, respectivamente, nas folhas bandeira e espigas de plantas não fornecidas com Si em comparação com plantas fornecidas por Si. A concentração de amido em espigas de plantas fornecidas com Si aumentou. Em conjunto, os resultados do presente estudo confirmam o papel ativo desempenhado pelo Si no aumento da resistência do trigo à brusone, mas apresenta novas evidências do efeito desse elemento em melhorar a relação fonte-dreno em folhas bandeiras e espigas infectadas preservando as alterações na produção de assimilados e partição durante o processo de enchimento de grãos.Universidade Federal de ViçosaRodrigues, Fabrício de Ávilahttp://lattes.cnpq.br/2773703667955616Rios, Jonas AlbertoAraujo, Marcela Uli Peixoto2019-11-21T18:27:24Z2019-11-21T18:27:24Z2017-07-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfARAUJO, Marcela Uli Peixoto. Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae. 2017. 44 f. Dissertação (Mestrado em Fitopatologia) - Universidade Federal de Viçosa, Viçosa. 2017.https://locus.ufv.br//handle/123456789/27457enginfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:57:24Zoai:locus.ufv.br:123456789/27457Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:57:24LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae
Silício alivia as mudanças na relação fonte-dreno em plantas de trigo infectadas por Pyricularia oryzae
title Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae
spellingShingle Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae
Araujo, Marcela Uli Peixoto
Brusone
Pyricularia oryzae
Triticum aestivum
Fotossíntese
Fitopatologia
title_short Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae
title_full Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae
title_fullStr Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae
title_full_unstemmed Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae
title_sort Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae
author Araujo, Marcela Uli Peixoto
author_facet Araujo, Marcela Uli Peixoto
author_role author
dc.contributor.none.fl_str_mv Rodrigues, Fabrício de Ávila
http://lattes.cnpq.br/2773703667955616
Rios, Jonas Alberto
dc.contributor.author.fl_str_mv Araujo, Marcela Uli Peixoto
dc.subject.por.fl_str_mv Brusone
Pyricularia oryzae
Triticum aestivum
Fotossíntese
Fitopatologia
topic Brusone
Pyricularia oryzae
Triticum aestivum
Fotossíntese
Fitopatologia
description Blast, caused by Pyricularia oryzae, has become an economically important disease on wheat in Brazil. Alternative methods for blast management are demanded by the growers and silicon (Si) stands out for its potential to decrease the intensities of important diseases in several crops. The present study aimed to investigate the effect of Si in improving the production of photoassimilates on flag leaves and their partitioning to spikes in a scenario where the blast symptoms will be lowered due to the potentiation of mechanisms of defense by this element on the source-sink interface. Wheat plants (cultivar BRS-Guamirim) were grown in hydroponic culture with either 0 or 2 mM Si and plants were inoculated with P. oryzae at 10 days after anthesis. The Si concentration on flag leaves and spikes of plants supplied with Si increased and contributed to decrease disease symptoms. Higher concentrations of total soluble phenolics and lignin-thioglycolic acid derivatives and greater activities of peroxidases, polyphenoloxidases, phenylalanine ammonia-lyases, β-1,3- glucanases and chitinases were noticed on flag leaves and spikes of plants supplied with Si and contributed to their resistance to blast. There was less concentration of pigments and an impairment of the photosynthetic performance of infected flag leaves and spikes from plants non-supplied with Si based on the values for the chlorophyll a fluorescence parameters maximal photosystem II quantum efficiency, fraction of energy absorbed used in photochemistry, quantum yield of non-regulated energy dissipation and quantum yield of regulated energy dissipation. On infected flag leaves and spikes from plants non-supplied with Si, the concentration of soluble sugars was lower while the hexoses-to-sucrose ratio increased on infected flag leaves. The sucrose phosphate synthase activity was lower while higher expression and activity of acid invertases was higher and lower, respectively, on flag leaves and spikes of plants non-supplied with Si in comparison to Si-supplied plants. The starch concentration on spikes of plants supplied with Si increased. All together, the results from the present study confirm the active role played by Si in increasing wheat resistance to blast, but present novel evidences of the effect of this element in improve the source-sink relationship on infected flag leaves and spikes by preserving the alterations in assimilate production and partitioning during the grain filling process.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-26
2019-11-21T18:27:24Z
2019-11-21T18:27:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv ARAUJO, Marcela Uli Peixoto. Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae. 2017. 44 f. Dissertação (Mestrado em Fitopatologia) - Universidade Federal de Viçosa, Viçosa. 2017.
https://locus.ufv.br//handle/123456789/27457
identifier_str_mv ARAUJO, Marcela Uli Peixoto. Silicon alleviates the changes in the source-sink relationship on wheat plants infected by Pyricularia oryzae. 2017. 44 f. Dissertação (Mestrado em Fitopatologia) - Universidade Federal de Viçosa, Viçosa. 2017.
url https://locus.ufv.br//handle/123456789/27457
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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