Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1099/mic.0.062901-0 http://www.locus.ufv.br/handle/123456789/19604 |
Resumo: | Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6–12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus. |
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Pereira, Jorge FernandoAlmeida, Ana Paula Morais MartinsCota, JúnioPamphile, João AlencarSilva, Gilvan Ferreira daAraújo, Elza Fernandes deGramacho, Karina PeresBrommonschenkel, Sérgio Hermı́nioPereira, Gonçalo Amarante GuimarãesQueiroz, Marisa Vieira de2018-05-16T12:10:59Z2018-05-16T12:10:59Z2012-10-2414652080https://doi.org/10.1099/mic.0.062901-0http://www.locus.ufv.br/handle/123456789/19604Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6–12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus.engMicrobiologyv. 159, p. 112-125, January 2013SGMinfo:eu-repo/semantics/openAccessMoniliophthora perniciosaPIF/HarbingerBoto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf921858https://locus.ufv.br//bitstream/123456789/19604/1/artigo.pdf07bb04848831f4beb04fdd97b5808dfaMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19604/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5085https://locus.ufv.br//bitstream/123456789/19604/3/artigo.pdf.jpge08a7abe2de5553508cc649ed5408fb6MD53123456789/196042018-05-16 23:01:07.034oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-05-17T02:01:07LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus |
title |
Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus |
spellingShingle |
Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus Pereira, Jorge Fernando Moniliophthora perniciosa PIF/Harbinger |
title_short |
Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus |
title_full |
Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus |
title_fullStr |
Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus |
title_full_unstemmed |
Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus |
title_sort |
Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus |
author |
Pereira, Jorge Fernando |
author_facet |
Pereira, Jorge Fernando Almeida, Ana Paula Morais Martins Cota, Júnio Pamphile, João Alencar Silva, Gilvan Ferreira da Araújo, Elza Fernandes de Gramacho, Karina Peres Brommonschenkel, Sérgio Hermı́nio Pereira, Gonçalo Amarante Guimarães Queiroz, Marisa Vieira de |
author_role |
author |
author2 |
Almeida, Ana Paula Morais Martins Cota, Júnio Pamphile, João Alencar Silva, Gilvan Ferreira da Araújo, Elza Fernandes de Gramacho, Karina Peres Brommonschenkel, Sérgio Hermı́nio Pereira, Gonçalo Amarante Guimarães Queiroz, Marisa Vieira de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pereira, Jorge Fernando Almeida, Ana Paula Morais Martins Cota, Júnio Pamphile, João Alencar Silva, Gilvan Ferreira da Araújo, Elza Fernandes de Gramacho, Karina Peres Brommonschenkel, Sérgio Hermı́nio Pereira, Gonçalo Amarante Guimarães Queiroz, Marisa Vieira de |
dc.subject.pt-BR.fl_str_mv |
Moniliophthora perniciosa PIF/Harbinger |
topic |
Moniliophthora perniciosa PIF/Harbinger |
description |
Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6–12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-10-24 |
dc.date.accessioned.fl_str_mv |
2018-05-16T12:10:59Z |
dc.date.available.fl_str_mv |
2018-05-16T12:10:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1099/mic.0.062901-0 http://www.locus.ufv.br/handle/123456789/19604 |
dc.identifier.issn.none.fl_str_mv |
14652080 |
identifier_str_mv |
14652080 |
url |
https://doi.org/10.1099/mic.0.062901-0 http://www.locus.ufv.br/handle/123456789/19604 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 159, p. 112-125, January 2013 |
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SGM info:eu-repo/semantics/openAccess |
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SGM |
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Microbiology |
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Microbiology |
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LOCUS Repositório Institucional da UFV |
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