Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | https://repositorio.unb.br/handle/10482/36338 https://doi.org/10.1016/j.bjp.2019.02.001 http://orcid.org/0000-0002-3393-5609 http://orcid.org/0000-0003-4392-4230 http://orcid.org/0000-0001-6098-3852 http://orcid.org/0000-0001-9599-4578 http://orcid.org/0000-0002-1503-1497 http://orcid.org/0000-0002-0531-7483 http://orcid.org/0000-0001-6072-7832 http://orcid.org/0000-0003-3401-290X http://orcid.org/0000-0003-1851-5224 http://orcid.org/0000-0003-3539-2916 |
Resumo: | Hippeastrum goianum (Ravenna) Meerow, Amaryllidaceae, is an endemic species from the Cerrado, Brazil; there are only few studies about its chemistry or biological activity. This study aimed to investigate the occurrence of lycorine in extracts from in vitro H. goianum plantlets, as well as evaluate a possible inhibition of acetylcholinesterase. The ethanol extract of plantlets produced by in vitro seed germination and micropropagation of bulblets was obtained from seedlings from in vitro germination, while the ethanol extract micropropagtion of bulblets was obtained from a subculture of those seedlings. The presence of lycorine was detected in only in the micropropagation of bulblets. The micropropagation of bulblets was more active than the plantlets produced by in vitro seed germination, with an IC50 of 114.8 ± 0.95 µg/ml and IC50 386.00 ± 0.97 µg/ml, respectively. These results showed that both in vitro germination and micropropagation of H. goianum can lead to the biosynthesis of lycorine. Moreover, the micropropagation led to improved anticholinesterase activity. |
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Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianumAlcalóidesBiossínteseCromatografia a líquidoLicorinaEspectrometria de massaHippeastrum goianum (Ravenna) Meerow, Amaryllidaceae, is an endemic species from the Cerrado, Brazil; there are only few studies about its chemistry or biological activity. This study aimed to investigate the occurrence of lycorine in extracts from in vitro H. goianum plantlets, as well as evaluate a possible inhibition of acetylcholinesterase. The ethanol extract of plantlets produced by in vitro seed germination and micropropagation of bulblets was obtained from seedlings from in vitro germination, while the ethanol extract micropropagtion of bulblets was obtained from a subculture of those seedlings. The presence of lycorine was detected in only in the micropropagation of bulblets. The micropropagation of bulblets was more active than the plantlets produced by in vitro seed germination, with an IC50 of 114.8 ± 0.95 µg/ml and IC50 386.00 ± 0.97 µg/ml, respectively. These results showed that both in vitro germination and micropropagation of H. goianum can lead to the biosynthesis of lycorine. Moreover, the micropropagation led to improved anticholinesterase activity.Sociedade Brasileira de Farmacognosia2020-01-24T10:30:21Z2020-01-24T10:30:21Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCOSTA, Gabriella Gusmão Pereira da et al. Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum. Revista Brasileira de Farmacognosia, v. 29, n. 2, p. 262-265, 2019. DOI: https://doi.org/10.1016/j.bjp.2019.02.001. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000200262. Acesso em: 23 jan.2020.https://repositorio.unb.br/handle/10482/36338https://doi.org/10.1016/j.bjp.2019.02.001http://orcid.org/0000-0002-3393-5609http://orcid.org/0000-0003-4392-4230http://orcid.org/0000-0001-6098-3852http://orcid.org/0000-0001-9599-4578http://orcid.org/0000-0002-1503-1497http://orcid.org/0000-0002-0531-7483http://orcid.org/0000-0001-6072-7832http://orcid.org/0000-0003-3401-290Xhttp://orcid.org/0000-0003-1851-5224http://orcid.org/0000-0003-3539-2916This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).info:eu-repo/semantics/openAccessCosta, Gabriella Gusmão Pereira daSilva, Cristian Aldemar GascaGomes, João Victor DutraTorres, Amanda GomesSantos, Izulmé Rita ImaculadaAlmeida, Fabrício Tavares Cunha deFagg, Christopher WilliamSimeoni, Luiz AlbertoSilveira, DâmarisGomes-Copeland, Kicia Karinne Pereiraengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-05-24T00:10:22Zoai:repositorio.unb.br:10482/36338Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T00:10:22Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.none.fl_str_mv |
Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
title |
Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
spellingShingle |
Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum Costa, Gabriella Gusmão Pereira da Alcalóides Biossíntese Cromatografia a líquido Licorina Espectrometria de massa |
title_short |
Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
title_full |
Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
title_fullStr |
Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
title_full_unstemmed |
Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
title_sort |
Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum |
author |
Costa, Gabriella Gusmão Pereira da |
author_facet |
Costa, Gabriella Gusmão Pereira da Silva, Cristian Aldemar Gasca Gomes, João Victor Dutra Torres, Amanda Gomes Santos, Izulmé Rita Imaculada Almeida, Fabrício Tavares Cunha de Fagg, Christopher William Simeoni, Luiz Alberto Silveira, Dâmaris Gomes-Copeland, Kicia Karinne Pereira |
author_role |
author |
author2 |
Silva, Cristian Aldemar Gasca Gomes, João Victor Dutra Torres, Amanda Gomes Santos, Izulmé Rita Imaculada Almeida, Fabrício Tavares Cunha de Fagg, Christopher William Simeoni, Luiz Alberto Silveira, Dâmaris Gomes-Copeland, Kicia Karinne Pereira |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Gabriella Gusmão Pereira da Silva, Cristian Aldemar Gasca Gomes, João Victor Dutra Torres, Amanda Gomes Santos, Izulmé Rita Imaculada Almeida, Fabrício Tavares Cunha de Fagg, Christopher William Simeoni, Luiz Alberto Silveira, Dâmaris Gomes-Copeland, Kicia Karinne Pereira |
dc.subject.por.fl_str_mv |
Alcalóides Biossíntese Cromatografia a líquido Licorina Espectrometria de massa |
topic |
Alcalóides Biossíntese Cromatografia a líquido Licorina Espectrometria de massa |
description |
Hippeastrum goianum (Ravenna) Meerow, Amaryllidaceae, is an endemic species from the Cerrado, Brazil; there are only few studies about its chemistry or biological activity. This study aimed to investigate the occurrence of lycorine in extracts from in vitro H. goianum plantlets, as well as evaluate a possible inhibition of acetylcholinesterase. The ethanol extract of plantlets produced by in vitro seed germination and micropropagation of bulblets was obtained from seedlings from in vitro germination, while the ethanol extract micropropagtion of bulblets was obtained from a subculture of those seedlings. The presence of lycorine was detected in only in the micropropagation of bulblets. The micropropagation of bulblets was more active than the plantlets produced by in vitro seed germination, with an IC50 of 114.8 ± 0.95 µg/ml and IC50 386.00 ± 0.97 µg/ml, respectively. These results showed that both in vitro germination and micropropagation of H. goianum can lead to the biosynthesis of lycorine. Moreover, the micropropagation led to improved anticholinesterase activity. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2020-01-24T10:30:21Z 2020-01-24T10:30:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
COSTA, Gabriella Gusmão Pereira da et al. Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum. Revista Brasileira de Farmacognosia, v. 29, n. 2, p. 262-265, 2019. DOI: https://doi.org/10.1016/j.bjp.2019.02.001. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000200262. Acesso em: 23 jan.2020. https://repositorio.unb.br/handle/10482/36338 https://doi.org/10.1016/j.bjp.2019.02.001 http://orcid.org/0000-0002-3393-5609 http://orcid.org/0000-0003-4392-4230 http://orcid.org/0000-0001-6098-3852 http://orcid.org/0000-0001-9599-4578 http://orcid.org/0000-0002-1503-1497 http://orcid.org/0000-0002-0531-7483 http://orcid.org/0000-0001-6072-7832 http://orcid.org/0000-0003-3401-290X http://orcid.org/0000-0003-1851-5224 http://orcid.org/0000-0003-3539-2916 |
identifier_str_mv |
COSTA, Gabriella Gusmão Pereira da et al. Influence of in vitro micropropagation on lycorine biosynthesis and anticholinesterase activity in Hippeastrum goianum. Revista Brasileira de Farmacognosia, v. 29, n. 2, p. 262-265, 2019. DOI: https://doi.org/10.1016/j.bjp.2019.02.001. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2019000200262. Acesso em: 23 jan.2020. |
url |
https://repositorio.unb.br/handle/10482/36338 https://doi.org/10.1016/j.bjp.2019.02.001 http://orcid.org/0000-0002-3393-5609 http://orcid.org/0000-0003-4392-4230 http://orcid.org/0000-0001-6098-3852 http://orcid.org/0000-0001-9599-4578 http://orcid.org/0000-0002-1503-1497 http://orcid.org/0000-0002-0531-7483 http://orcid.org/0000-0001-6072-7832 http://orcid.org/0000-0003-3401-290X http://orcid.org/0000-0003-1851-5224 http://orcid.org/0000-0003-3539-2916 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
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reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
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Universidade de Brasília (UnB) |
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UNB |
institution |
UNB |
reponame_str |
Repositório Institucional da UnB |
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Repositório Institucional da UnB |
repository.name.fl_str_mv |
Repositório Institucional da UnB - Universidade de Brasília (UnB) |
repository.mail.fl_str_mv |
repositorio@unb.br |
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1810580894257250304 |