Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus

Detalhes bibliográficos
Autor(a) principal: Vieira, Michele Juliane
Data de Publicação: 2014
Outros Autores: Perosa, Sandra Regina, Argañaraz, Gustavo Adolfo, Silva Junior, José Antônio, Cavalheiro, Esper Abrão, Naffah-Mazzacoratti, Maria da Graça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/29416
https://dx.doi.org/10.6061/clinics/2014(09)08
Resumo: OBJECTIVE: Refractory status epilepticus is one of the most life-threatening neurological emergencies and is characterized by high morbidity and mortality. Additionally, the use of anti-inflammatory drugs during this period is very controversial. Thus, this study has been designed to analyze the effect of a low dose of indomethacin (a COX inhibitor) on the expression of inflammatory molecules. METHOD: The hippocampus of rats submitted to pilocarpine-induced long-lasting status epilepticus was analyzed to determine the expression of inflammatory molecules with RT-PCR and immunohistochemistry. RESULTS: Compared with controls, reduced levels of the kinin B2 receptors IL1β and TNFα were found in the hippocampus of rats submitted to long-lasting status epilepticus and treated with indomethacin. CONCLUSIONS: These data show that low doses of indomethacin could be employed to minimize inflammation during long-lasting status epilepticus.
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spelling Vieira, Michele JulianePerosa, Sandra ReginaArgañaraz, Gustavo AdolfoSilva Junior, José AntônioCavalheiro, Esper AbrãoNaffah-Mazzacoratti, Maria da Graça2017-12-07T05:09:09Z2017-12-07T05:09:09Z2014VIEIRA, Michele Juliane et al. Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus. Clinics, São Paulo, v. 69, n. 9, p. 621-626, set. 2014. DOI: http://dx.doi.org/10.6061/clinics/2014(09)08. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322014000900621&lng=en&nrm=iso. Acesso em: 30 out. 2019. http://repositorio.unb.br/handle/10482/29416https://dx.doi.org/10.6061/clinics/2014(09)08Faculdade de Medicina / USPClinics - (CC BY NC) - This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322014000900621&lng=en&nrm=iso&tlng=en. Acesso em: 30 out. 2019.info:eu-repo/semantics/openAccessIndomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnti-inflamatóriosInflamaçãoEpilepsiaOBJECTIVE: Refractory status epilepticus is one of the most life-threatening neurological emergencies and is characterized by high morbidity and mortality. Additionally, the use of anti-inflammatory drugs during this period is very controversial. Thus, this study has been designed to analyze the effect of a low dose of indomethacin (a COX inhibitor) on the expression of inflammatory molecules. METHOD: The hippocampus of rats submitted to pilocarpine-induced long-lasting status epilepticus was analyzed to determine the expression of inflammatory molecules with RT-PCR and immunohistochemistry. RESULTS: Compared with controls, reduced levels of the kinin B2 receptors IL1β and TNFα were found in the hippocampus of rats submitted to long-lasting status epilepticus and treated with indomethacin. CONCLUSIONS: These data show that low doses of indomethacin could be employed to minimize inflammation during long-lasting status epilepticus.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_ IndomethacinDownregulateLevels.pdfARTIGO_ IndomethacinDownregulateLevels.pdfapplication/pdf2444073http://repositorio2.unb.br/jspui/bitstream/10482/29416/1/ARTIGO_%20IndomethacinDownregulateLevels.pdffddd3a0e1495d00437926ce7d2b097ecMD51open access10482/294162023-05-24 20:08:58.799open accessoai:repositorio2.unb.br:10482/29416Biblioteca Digital de Teses e DissertaçõesPUBhttps://repositorio.unb.br/oai/requestopendoar:2023-05-24T23:08:58Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.pt_BR.fl_str_mv Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
title Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
spellingShingle Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
Vieira, Michele Juliane
Anti-inflamatórios
Inflamação
Epilepsia
title_short Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
title_full Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
title_fullStr Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
title_full_unstemmed Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
title_sort Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
author Vieira, Michele Juliane
author_facet Vieira, Michele Juliane
Perosa, Sandra Regina
Argañaraz, Gustavo Adolfo
Silva Junior, José Antônio
Cavalheiro, Esper Abrão
Naffah-Mazzacoratti, Maria da Graça
author_role author
author2 Perosa, Sandra Regina
Argañaraz, Gustavo Adolfo
Silva Junior, José Antônio
Cavalheiro, Esper Abrão
Naffah-Mazzacoratti, Maria da Graça
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Michele Juliane
Perosa, Sandra Regina
Argañaraz, Gustavo Adolfo
Silva Junior, José Antônio
Cavalheiro, Esper Abrão
Naffah-Mazzacoratti, Maria da Graça
dc.subject.keyword.pt_BR.fl_str_mv Anti-inflamatórios
Inflamação
Epilepsia
topic Anti-inflamatórios
Inflamação
Epilepsia
description OBJECTIVE: Refractory status epilepticus is one of the most life-threatening neurological emergencies and is characterized by high morbidity and mortality. Additionally, the use of anti-inflammatory drugs during this period is very controversial. Thus, this study has been designed to analyze the effect of a low dose of indomethacin (a COX inhibitor) on the expression of inflammatory molecules. METHOD: The hippocampus of rats submitted to pilocarpine-induced long-lasting status epilepticus was analyzed to determine the expression of inflammatory molecules with RT-PCR and immunohistochemistry. RESULTS: Compared with controls, reduced levels of the kinin B2 receptors IL1β and TNFα were found in the hippocampus of rats submitted to long-lasting status epilepticus and treated with indomethacin. CONCLUSIONS: These data show that low doses of indomethacin could be employed to minimize inflammation during long-lasting status epilepticus.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2017-12-07T05:09:09Z
dc.date.available.fl_str_mv 2017-12-07T05:09:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIEIRA, Michele Juliane et al. Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus. Clinics, São Paulo, v. 69, n. 9, p. 621-626, set. 2014. DOI: http://dx.doi.org/10.6061/clinics/2014(09)08. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322014000900621&lng=en&nrm=iso. Acesso em: 30 out. 2019. 
dc.identifier.uri.fl_str_mv http://repositorio.unb.br/handle/10482/29416
dc.identifier.doi.pt_BR.fl_str_mv https://dx.doi.org/10.6061/clinics/2014(09)08
identifier_str_mv VIEIRA, Michele Juliane et al. Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus. Clinics, São Paulo, v. 69, n. 9, p. 621-626, set. 2014. DOI: http://dx.doi.org/10.6061/clinics/2014(09)08. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322014000900621&lng=en&nrm=iso. Acesso em: 30 out. 2019. 
url http://repositorio.unb.br/handle/10482/29416
https://dx.doi.org/10.6061/clinics/2014(09)08
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Faculdade de Medicina / USP
publisher.none.fl_str_mv Faculdade de Medicina / USP
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
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reponame_str Repositório Institucional da UnB
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