Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil

Detalhes bibliográficos
Autor(a) principal: Silva, Aline Suelen da
Data de Publicação: 2023
Outros Autores: Rennó, Marcelo Henrique Lisboa, Quitania, Ana Clara Ribeiro, Café‑Filho, Adalberto Corrêa, Miller, Robert NeilGerard, Araújo, Alderi Emidio de, Pinho, Danilo Batista
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio2.unb.br/jspui/handle/10482/46863
https://doi.org/10.1038/s41598-023-33530-3
https://orcid.org/0000-0002-0550-5847
https://orcid.org/0000-0001-5195-9930
https://orcid.org/0000-0002-8166-7127
https://orcid.org/0000-0002-5204-2961
https://orcid.org/0000-0002-5798-4552
https://orcid.org/0000-0003-0257-1539
https://orcid.org/0000-0003-2624-302X
Resumo: Whilst Brazil is the fourth largest cotton producer globally, incidence of ramularia leaf spot (RLS) has decreased yield. In 2017–18 and 2018–19, ca. 300 fungal samples were collected throughout Brazil. Hyphal tip cultures were obtained for amplification of the RNA polymerase II (RPB2), 28S rRNA, the ribosomal DNA internal transcribed spacers (ITS), actin (ACT), elongation factor (EF1-α) and histone H3 (HIS3) genomic regions. Additionally, sequences of the glyceraldehyde‑3‑phosphate dehydrogenase (GAPDH) were obtained by nanopore sequencing and the EF1-α region was selected as a marker for rapid recognition of Ramulariopsis species. Clade assignments based on the concatenated‑sequence tree were identical to those in tree generated by RPB2‑sequences, as well as in an RPB2 haplotype network and an ISSR (TGTC) 4 dendrogram, in identification with species‑specific primers and based on morphological comparisons. Out of 267 examined isolates, 252 were identified as Ramulariopsis pseudoglycines, indicating this species as the most widespread causal agent of cotton RLS in the Brazilian growing regions. Species‑specific primers developed in the study that target the EF1-α gene provide an opportunity for extensive RLS sampling worldwide to study the distribution of Ramulariopsis species. Such data will aid breeders and plant pathologists in cotton disease resistance development and fungicide resistance avoidance.
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spelling Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in BrazilWhilst Brazil is the fourth largest cotton producer globally, incidence of ramularia leaf spot (RLS) has decreased yield. In 2017–18 and 2018–19, ca. 300 fungal samples were collected throughout Brazil. Hyphal tip cultures were obtained for amplification of the RNA polymerase II (RPB2), 28S rRNA, the ribosomal DNA internal transcribed spacers (ITS), actin (ACT), elongation factor (EF1-α) and histone H3 (HIS3) genomic regions. Additionally, sequences of the glyceraldehyde‑3‑phosphate dehydrogenase (GAPDH) were obtained by nanopore sequencing and the EF1-α region was selected as a marker for rapid recognition of Ramulariopsis species. Clade assignments based on the concatenated‑sequence tree were identical to those in tree generated by RPB2‑sequences, as well as in an RPB2 haplotype network and an ISSR (TGTC) 4 dendrogram, in identification with species‑specific primers and based on morphological comparisons. Out of 267 examined isolates, 252 were identified as Ramulariopsis pseudoglycines, indicating this species as the most widespread causal agent of cotton RLS in the Brazilian growing regions. Species‑specific primers developed in the study that target the EF1-α gene provide an opportunity for extensive RLS sampling worldwide to study the distribution of Ramulariopsis species. Such data will aid breeders and plant pathologists in cotton disease resistance development and fungicide resistance avoidance.Instituto de Ciências Biológicas (IB)Departamento de Fitopatologia (IB FIT)Departamento de Biologia Celular (IB CEL)Springer NatureUniversidade de BrasíliaUniversidade de BrasíliaUniversidade de BrasíliaUniversidade de BrasíliaUniversidade de BrasíliaEmbrapa Algodão, Campina GrandeUniversidade de BrasíliaSilva, Aline Suelen daRennó, Marcelo Henrique LisboaQuitania, Ana Clara RibeiroCafé‑Filho, Adalberto CorrêaMiller, Robert NeilGerardAraújo, Alderi Emidio dePinho, Danilo Batista2023-11-15T14:04:35Z2023-11-15T14:04:35Z2023-06-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA,a line Suelen da et al. Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil. Scientific Reports, v. 13, 9826, 2023. DOI: https://doi.org/10.1038/s41598-023-33530-3. Disponível em: https://www.nature.com/articles/s41598-023-33530-3. Acesso em: 15 nov. 2023.http://repositorio2.unb.br/jspui/handle/10482/46863https://doi.org/10.1038/s41598-023-33530-3https://orcid.org/0000-0002-0550-5847https://orcid.org/0000-0001-5195-9930https://orcid.org/0000-0002-8166-7127https://orcid.org/0000-0002-5204-2961https://orcid.org/0000-0002-5798-4552https://orcid.org/0000-0003-0257-1539https://orcid.org/0000-0003-2624-302XengOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creativecommons. org/ licenses/ by/4. 0/.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-11-15T14:04:36Zoai:repositorio.unb.br:10482/46863Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-11-15T14:04:36Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
title Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
spellingShingle Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
Silva, Aline Suelen da
title_short Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
title_full Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
title_fullStr Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
title_full_unstemmed Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
title_sort Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
author Silva, Aline Suelen da
author_facet Silva, Aline Suelen da
Rennó, Marcelo Henrique Lisboa
Quitania, Ana Clara Ribeiro
Café‑Filho, Adalberto Corrêa
Miller, Robert NeilGerard
Araújo, Alderi Emidio de
Pinho, Danilo Batista
author_role author
author2 Rennó, Marcelo Henrique Lisboa
Quitania, Ana Clara Ribeiro
Café‑Filho, Adalberto Corrêa
Miller, Robert NeilGerard
Araújo, Alderi Emidio de
Pinho, Danilo Batista
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Brasília
Universidade de Brasília
Universidade de Brasília
Universidade de Brasília
Universidade de Brasília
Embrapa Algodão, Campina Grande
Universidade de Brasília
dc.contributor.author.fl_str_mv Silva, Aline Suelen da
Rennó, Marcelo Henrique Lisboa
Quitania, Ana Clara Ribeiro
Café‑Filho, Adalberto Corrêa
Miller, Robert NeilGerard
Araújo, Alderi Emidio de
Pinho, Danilo Batista
description Whilst Brazil is the fourth largest cotton producer globally, incidence of ramularia leaf spot (RLS) has decreased yield. In 2017–18 and 2018–19, ca. 300 fungal samples were collected throughout Brazil. Hyphal tip cultures were obtained for amplification of the RNA polymerase II (RPB2), 28S rRNA, the ribosomal DNA internal transcribed spacers (ITS), actin (ACT), elongation factor (EF1-α) and histone H3 (HIS3) genomic regions. Additionally, sequences of the glyceraldehyde‑3‑phosphate dehydrogenase (GAPDH) were obtained by nanopore sequencing and the EF1-α region was selected as a marker for rapid recognition of Ramulariopsis species. Clade assignments based on the concatenated‑sequence tree were identical to those in tree generated by RPB2‑sequences, as well as in an RPB2 haplotype network and an ISSR (TGTC) 4 dendrogram, in identification with species‑specific primers and based on morphological comparisons. Out of 267 examined isolates, 252 were identified as Ramulariopsis pseudoglycines, indicating this species as the most widespread causal agent of cotton RLS in the Brazilian growing regions. Species‑specific primers developed in the study that target the EF1-α gene provide an opportunity for extensive RLS sampling worldwide to study the distribution of Ramulariopsis species. Such data will aid breeders and plant pathologists in cotton disease resistance development and fungicide resistance avoidance.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-15T14:04:35Z
2023-11-15T14:04:35Z
2023-06-17
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA,a line Suelen da et al. Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil. Scientific Reports, v. 13, 9826, 2023. DOI: https://doi.org/10.1038/s41598-023-33530-3. Disponível em: https://www.nature.com/articles/s41598-023-33530-3. Acesso em: 15 nov. 2023.
http://repositorio2.unb.br/jspui/handle/10482/46863
https://doi.org/10.1038/s41598-023-33530-3
https://orcid.org/0000-0002-0550-5847
https://orcid.org/0000-0001-5195-9930
https://orcid.org/0000-0002-8166-7127
https://orcid.org/0000-0002-5204-2961
https://orcid.org/0000-0002-5798-4552
https://orcid.org/0000-0003-0257-1539
https://orcid.org/0000-0003-2624-302X
identifier_str_mv SILVA,a line Suelen da et al. Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil. Scientific Reports, v. 13, 9826, 2023. DOI: https://doi.org/10.1038/s41598-023-33530-3. Disponível em: https://www.nature.com/articles/s41598-023-33530-3. Acesso em: 15 nov. 2023.
url http://repositorio2.unb.br/jspui/handle/10482/46863
https://doi.org/10.1038/s41598-023-33530-3
https://orcid.org/0000-0002-0550-5847
https://orcid.org/0000-0001-5195-9930
https://orcid.org/0000-0002-8166-7127
https://orcid.org/0000-0002-5204-2961
https://orcid.org/0000-0002-5798-4552
https://orcid.org/0000-0003-0257-1539
https://orcid.org/0000-0003-2624-302X
dc.language.iso.fl_str_mv eng
language eng
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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