Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio.unb.br/handle/10482/29528 http://dx.doi.org/10.1590/0037-8682-0132-2015 |
Resumo: | Introduction: in the Americas, mucosal leishmaniasis is primarily associated with infection by Leishmania (Viannia) braziliensis. However, Leishmania (Viannia) guyanensis is another important cause of this disease in the Brazilian Amazon. In this study, we aimed at detecting Leishmaniadeoxyribonucleic acid (DNA) within paraffin-embedded fragments of mucosal tissues, and characterizing the infecting parasite species. Methods: we evaluated samples collected from 114 patients treated at a reference center in the Brazilian Amazon by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses. Results: direct examination of biopsy imprints detected parasites in 10 of the 114 samples, while evaluation of hematoxylin and eosin-stained slides detected amastigotes in an additional 17 samples. Meanwhile, 31/114 samples (27.2%) were positive for Leishmania spp. kinetoplast deoxyribonucleic acid (kDNA) by PCR analysis. Of these, 17 (54.8%) yielded amplification of the mini-exon PCR target, thereby allowing for PCR-RFLP-based identification. Six of the samples were identified as L. (V.) braziliensis, while the remaining 11 were identified as L. (V.) guyanensis. Conclusions: the results of this study demonstrate the feasibility of applying molecular techniques for the diagnosis of human parasites within paraffin-embedded tissues. Moreover, our findings confirm that L. (V.) guyanensisis a relevant causative agent of mucosal leishmaniasis in the Brazilian Amazon. |
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Prestes, Suzane RibeiroGuerra, Jorge Augusto de OliveiraRomero, Gustavo Adolfo SierraMagalhães, Laylah Kelre CostaSantana, Rosa Amelia GonçalvesMaciel, Marcel GonçalvesCustódio, AnaBarbosa, Maria das Graças ValeSilveira, Henrique2017-12-07T05:11:35Z2017-12-07T05:11:35Z2015-09PRESTES, Suzane Ribeiro et al. Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 48, n. 5, p. 555-559, set./out. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500555&lng=en&nrm=iso>. Acesso em: 10 maio 2018. doi: http://dx.doi.org/10.1590/0037-8682-0132-2015.http://repositorio.unb.br/handle/10482/29528http://dx.doi.org/10.1590/0037-8682-0132-2015Sociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical - This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500555&lng=en&nrm=iso. Acesso em: 10 maio 2018.info:eu-repo/semantics/openAccessPolymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmanioseDiagnóstico molecularIntroduction: in the Americas, mucosal leishmaniasis is primarily associated with infection by Leishmania (Viannia) braziliensis. However, Leishmania (Viannia) guyanensis is another important cause of this disease in the Brazilian Amazon. In this study, we aimed at detecting Leishmaniadeoxyribonucleic acid (DNA) within paraffin-embedded fragments of mucosal tissues, and characterizing the infecting parasite species. Methods: we evaluated samples collected from 114 patients treated at a reference center in the Brazilian Amazon by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses. Results: direct examination of biopsy imprints detected parasites in 10 of the 114 samples, while evaluation of hematoxylin and eosin-stained slides detected amastigotes in an additional 17 samples. Meanwhile, 31/114 samples (27.2%) were positive for Leishmania spp. kinetoplast deoxyribonucleic acid (kDNA) by PCR analysis. Of these, 17 (54.8%) yielded amplification of the mini-exon PCR target, thereby allowing for PCR-RFLP-based identification. Six of the samples were identified as L. (V.) braziliensis, while the remaining 11 were identified as L. (V.) guyanensis. Conclusions: the results of this study demonstrate the feasibility of applying molecular techniques for the diagnosis of human parasites within paraffin-embedded tissues. Moreover, our findings confirm that L. (V.) guyanensisis a relevant causative agent of mucosal leishmaniasis in the Brazilian Amazon.Faculdade de Medicina (FMD)engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_PolymeraseChainReaction-based.pdfARTIGO_PolymeraseChainReaction-based.pdfapplication/pdf690222http://repositorio2.unb.br/jspui/bitstream/10482/29528/1/ARTIGO_PolymeraseChainReaction-based.pdfc12bd9c62b81e711b99d65f8426d8dfaMD51open access10482/295282023-08-25 15:42:42.094open accessoai:repositorio2.unb.br:10482/29528Biblioteca Digital de Teses e DissertaçõesPUBhttps://repositorio.unb.br/oai/requestopendoar:2023-08-25T18:42:42Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.pt_BR.fl_str_mv |
Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin |
title |
Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin |
spellingShingle |
Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin Prestes, Suzane Ribeiro Leishmaniose Diagnóstico molecular |
title_short |
Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin |
title_full |
Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin |
title_fullStr |
Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin |
title_full_unstemmed |
Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin |
title_sort |
Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin |
author |
Prestes, Suzane Ribeiro |
author_facet |
Prestes, Suzane Ribeiro Guerra, Jorge Augusto de Oliveira Romero, Gustavo Adolfo Sierra Magalhães, Laylah Kelre Costa Santana, Rosa Amelia Gonçalves Maciel, Marcel Gonçalves Custódio, Ana Barbosa, Maria das Graças Vale Silveira, Henrique |
author_role |
author |
author2 |
Guerra, Jorge Augusto de Oliveira Romero, Gustavo Adolfo Sierra Magalhães, Laylah Kelre Costa Santana, Rosa Amelia Gonçalves Maciel, Marcel Gonçalves Custódio, Ana Barbosa, Maria das Graças Vale Silveira, Henrique |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Prestes, Suzane Ribeiro Guerra, Jorge Augusto de Oliveira Romero, Gustavo Adolfo Sierra Magalhães, Laylah Kelre Costa Santana, Rosa Amelia Gonçalves Maciel, Marcel Gonçalves Custódio, Ana Barbosa, Maria das Graças Vale Silveira, Henrique |
dc.subject.keyword.pt_BR.fl_str_mv |
Leishmaniose Diagnóstico molecular |
topic |
Leishmaniose Diagnóstico molecular |
description |
Introduction: in the Americas, mucosal leishmaniasis is primarily associated with infection by Leishmania (Viannia) braziliensis. However, Leishmania (Viannia) guyanensis is another important cause of this disease in the Brazilian Amazon. In this study, we aimed at detecting Leishmaniadeoxyribonucleic acid (DNA) within paraffin-embedded fragments of mucosal tissues, and characterizing the infecting parasite species. Methods: we evaluated samples collected from 114 patients treated at a reference center in the Brazilian Amazon by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses. Results: direct examination of biopsy imprints detected parasites in 10 of the 114 samples, while evaluation of hematoxylin and eosin-stained slides detected amastigotes in an additional 17 samples. Meanwhile, 31/114 samples (27.2%) were positive for Leishmania spp. kinetoplast deoxyribonucleic acid (kDNA) by PCR analysis. Of these, 17 (54.8%) yielded amplification of the mini-exon PCR target, thereby allowing for PCR-RFLP-based identification. Six of the samples were identified as L. (V.) braziliensis, while the remaining 11 were identified as L. (V.) guyanensis. Conclusions: the results of this study demonstrate the feasibility of applying molecular techniques for the diagnosis of human parasites within paraffin-embedded tissues. Moreover, our findings confirm that L. (V.) guyanensisis a relevant causative agent of mucosal leishmaniasis in the Brazilian Amazon. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-09 |
dc.date.accessioned.fl_str_mv |
2017-12-07T05:11:35Z |
dc.date.available.fl_str_mv |
2017-12-07T05:11:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PRESTES, Suzane Ribeiro et al. Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 48, n. 5, p. 555-559, set./out. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500555&lng=en&nrm=iso>. Acesso em: 10 maio 2018. doi: http://dx.doi.org/10.1590/0037-8682-0132-2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unb.br/handle/10482/29528 |
dc.identifier.doi.pt_BR.fl_str_mv |
http://dx.doi.org/10.1590/0037-8682-0132-2015 |
identifier_str_mv |
PRESTES, Suzane Ribeiro et al. Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 48, n. 5, p. 555-559, set./out. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500555&lng=en&nrm=iso>. Acesso em: 10 maio 2018. doi: http://dx.doi.org/10.1590/0037-8682-0132-2015. |
url |
http://repositorio.unb.br/handle/10482/29528 http://dx.doi.org/10.1590/0037-8682-0132-2015 |
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eng |
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eng |
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Sociedade Brasileira de Medicina Tropical - SBMT |
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Sociedade Brasileira de Medicina Tropical - SBMT |
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reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
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UNB |
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UNB |
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Repositório Institucional da UnB |
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http://repositorio2.unb.br/jspui/bitstream/10482/29528/1/ARTIGO_PolymeraseChainReaction-based.pdf |
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