Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil

Detalhes bibliográficos
Autor(a) principal: Aranha, Silvia de Araujo
Data de Publicação: 2011
Outros Autores: Albuquerque, Leonardo Cunha de, Boiteux, Leonardo Silva, Nagata, Alice Kazuko Inoue
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/28238
https://dx.doi.org/10.1590/S1982-56762011000100002
Resumo: A survey of okra begomoviruses was carried out in Central Brazil. Foliar samples were collected in okra production fields and tested by using begomovirus universal primers. Begomovirus infection was confirmed in only one (#5157) out of 196 samples. Total DNA was subjected to PCR amplification and introduced into okra seedlings by a biolistic method; the bombarded DNA sample was infectious to okra plants. The DNA-A and DNA-B of isolate #5157 were cloned and their nucleotide sequences exhibited typical characteristics of New World bipartite begomoviruses. The DNA-A sequence shared 95.6% nucleotide identity with an isolate of Sida micrantha mosaic virus from Brazil and thus identified as its okra strain. The clones derived from #5157 were infectious to okra, Sida santaremnensis and to a group of Solanaceae plants when inoculated by biolistics after circularization of the isolated insert, followed by rolling circle amplification.
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spelling Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in BrazilDetecção e caracterização do genoma completo de um begomovírus que infecta o quiabeiro (Abelmoschus esculentus) no BrasilVírus do mosaico Sida MicranthaGeminivírussimmvA survey of okra begomoviruses was carried out in Central Brazil. Foliar samples were collected in okra production fields and tested by using begomovirus universal primers. Begomovirus infection was confirmed in only one (#5157) out of 196 samples. Total DNA was subjected to PCR amplification and introduced into okra seedlings by a biolistic method; the bombarded DNA sample was infectious to okra plants. The DNA-A and DNA-B of isolate #5157 were cloned and their nucleotide sequences exhibited typical characteristics of New World bipartite begomoviruses. The DNA-A sequence shared 95.6% nucleotide identity with an isolate of Sida micrantha mosaic virus from Brazil and thus identified as its okra strain. The clones derived from #5157 were infectious to okra, Sida santaremnensis and to a group of Solanaceae plants when inoculated by biolistics after circularization of the isolated insert, followed by rolling circle amplification.Um levantamento de begomovírus de quiabeiro foi realizado no Brasil Central. Amostras foliares foram coletadas em campos de produção de quiabo e avaliadas em testes utilizando primers universais para begomovírus. A infecção por begomovírus foi confirmada em apenas uma amostra (#5157) de um total de 196 amostras. O DNA total foi submetido à amplificação por PCR e introduzido em plântulas de quiabeiro pelo método de biobalística, sendo que a amostra de DNA bombardeada foi infecciosa em plantas de quiabeiro. O DNA-A e DNA-B do isolado #5157 foram clonados e a sequência de nucleotídeos mostrou características típicas de begomovírus do Novo Mundo. A sequência do DNA-A apresentou 95,6% de identidade nucleotídica com um isolado de Sida micrantha mosaic virus do Brasil, sendo assim identificado como sua estirpe de quiabeiro. Os clones gerados a partir da amostra #5157 foram infecciosos para quiabeiro, Sida santaremnensis e em um grupo de plantas solanáceas quando inoculados por biobalística após circularização do inserto isolado, seguido por amplificação por círculo rolante.Em processamentoSociedade Brasileira de Fitopatologia2017-12-07T04:57:33Z2017-12-07T04:57:33Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfTrop. plant pathol.,v.36,n.1,p.14-20,2011http://repositorio.unb.br/handle/10482/28238https://dx.doi.org/10.1590/S1982-56762011000100002Aranha, Silvia de AraujoAlbuquerque, Leonardo Cunha deBoiteux, Leonardo SilvaNagata, Alice Kazuko Inoueinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T19:04:08Zoai:repositorio.unb.br:10482/28238Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:08Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
Detecção e caracterização do genoma completo de um begomovírus que infecta o quiabeiro (Abelmoschus esculentus) no Brasil
title Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
spellingShingle Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
Aranha, Silvia de Araujo
Vírus do mosaico Sida Micrantha
Geminivírus
simmv
title_short Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
title_full Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
title_fullStr Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
title_full_unstemmed Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
title_sort Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
author Aranha, Silvia de Araujo
author_facet Aranha, Silvia de Araujo
Albuquerque, Leonardo Cunha de
Boiteux, Leonardo Silva
Nagata, Alice Kazuko Inoue
author_role author
author2 Albuquerque, Leonardo Cunha de
Boiteux, Leonardo Silva
Nagata, Alice Kazuko Inoue
author2_role author
author
author
dc.contributor.author.fl_str_mv Aranha, Silvia de Araujo
Albuquerque, Leonardo Cunha de
Boiteux, Leonardo Silva
Nagata, Alice Kazuko Inoue
dc.subject.por.fl_str_mv Vírus do mosaico Sida Micrantha
Geminivírus
simmv
topic Vírus do mosaico Sida Micrantha
Geminivírus
simmv
description A survey of okra begomoviruses was carried out in Central Brazil. Foliar samples were collected in okra production fields and tested by using begomovirus universal primers. Begomovirus infection was confirmed in only one (#5157) out of 196 samples. Total DNA was subjected to PCR amplification and introduced into okra seedlings by a biolistic method; the bombarded DNA sample was infectious to okra plants. The DNA-A and DNA-B of isolate #5157 were cloned and their nucleotide sequences exhibited typical characteristics of New World bipartite begomoviruses. The DNA-A sequence shared 95.6% nucleotide identity with an isolate of Sida micrantha mosaic virus from Brazil and thus identified as its okra strain. The clones derived from #5157 were infectious to okra, Sida santaremnensis and to a group of Solanaceae plants when inoculated by biolistics after circularization of the isolated insert, followed by rolling circle amplification.
publishDate 2011
dc.date.none.fl_str_mv 2011
2017-12-07T04:57:33Z
2017-12-07T04:57:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Trop. plant pathol.,v.36,n.1,p.14-20,2011
http://repositorio.unb.br/handle/10482/28238
https://dx.doi.org/10.1590/S1982-56762011000100002
identifier_str_mv Trop. plant pathol.,v.36,n.1,p.14-20,2011
url http://repositorio.unb.br/handle/10482/28238
https://dx.doi.org/10.1590/S1982-56762011000100002
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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