Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio.unb.br/handle/10482/29441 https://dx.doi.org/10.1590/S1982-56762014000500005 |
Resumo: | Biological control of seed-borne pathogens has shown to enhance germination and physiological quality of seeds. The objectives of this study were to evaluate the in vitro antagonistic effect of five Trichoderma harzianum isolates (CEN287, CEN288, CEN289, CEN290, and CEN316) against Fusarium oxysporum f. sp. phaseoli (Foxy) and test its potential use in seed treatment. Initially, pathogen and antagonists were grown in paired cultures at 25ºC, from which samples were assessed using scanning electron microscopy (SEM). Then, clean or Foxy-infected seeds were treated with conidial suspension of the antagonists. Percent of Foxy-infected seeds and normal seedlings were evaluated at seven and nine days of incubation, respectively. All but one Trichoderma isolate (CEN290) inhibited Foxy mycelial growth. SEM analysis revealed that only one Trichoderma isolate (CEN287) showed parasitic interaction with Foxy. Two isolates (CEN287 and CEN316) significantly reduced the Foxy incidence and enhanced seed germination, though less effective than the fungicide mixture (carboxin + thiram). A principal component analysis indicated the importance of volatile metabolites in reducing Foxy incidence on common bean seeds. CEN287 Trichoderma harzianum isolate formed a single group due to its increase in germination rate of Foxyinfected seeds. |
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Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatmentFeijãoSementesControle biológicoBiological control of seed-borne pathogens has shown to enhance germination and physiological quality of seeds. The objectives of this study were to evaluate the in vitro antagonistic effect of five Trichoderma harzianum isolates (CEN287, CEN288, CEN289, CEN290, and CEN316) against Fusarium oxysporum f. sp. phaseoli (Foxy) and test its potential use in seed treatment. Initially, pathogen and antagonists were grown in paired cultures at 25ºC, from which samples were assessed using scanning electron microscopy (SEM). Then, clean or Foxy-infected seeds were treated with conidial suspension of the antagonists. Percent of Foxy-infected seeds and normal seedlings were evaluated at seven and nine days of incubation, respectively. All but one Trichoderma isolate (CEN290) inhibited Foxy mycelial growth. SEM analysis revealed that only one Trichoderma isolate (CEN287) showed parasitic interaction with Foxy. Two isolates (CEN287 and CEN316) significantly reduced the Foxy incidence and enhanced seed germination, though less effective than the fungicide mixture (carboxin + thiram). A principal component analysis indicated the importance of volatile metabolites in reducing Foxy incidence on common bean seeds. CEN287 Trichoderma harzianum isolate formed a single group due to its increase in germination rate of Foxyinfected seeds.Sociedade Brasileira de Fitopatologia2017-12-07T05:09:13Z2017-12-07T05:09:13Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCARVALHO, Daniel D. C. et al. Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment. Tropical Plant Pathology, Brasília, v. 39, n. 5, p. 384-391, out. 2014. DOI: http://dx.doi.org/10.1590/S1982-56762014000500005. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000500005&lng=en&nrm=iso. Acesso em: 30 out. 2019.http://repositorio.unb.br/handle/10482/29441https://dx.doi.org/10.1590/S1982-56762014000500005Tropical Plant Pathology - (CC BY NC) - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000500005&lng=en&nrm=iso&tlng=. Acesso em: 30 out. 2019.info:eu-repo/semantics/openAccessCarvalho, Daniel Diego CostaLobo Junior, MurilloMartins, IreneInglis, Peter W.Mello, Sueli Corrêa Marques deengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-05-24T23:45:10Zoai:repositorio.unb.br:10482/29441Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:45:10Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.none.fl_str_mv |
Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment |
title |
Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment |
spellingShingle |
Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment Carvalho, Daniel Diego Costa Feijão Sementes Controle biológico |
title_short |
Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment |
title_full |
Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment |
title_fullStr |
Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment |
title_full_unstemmed |
Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment |
title_sort |
Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment |
author |
Carvalho, Daniel Diego Costa |
author_facet |
Carvalho, Daniel Diego Costa Lobo Junior, Murillo Martins, Irene Inglis, Peter W. Mello, Sueli Corrêa Marques de |
author_role |
author |
author2 |
Lobo Junior, Murillo Martins, Irene Inglis, Peter W. Mello, Sueli Corrêa Marques de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Carvalho, Daniel Diego Costa Lobo Junior, Murillo Martins, Irene Inglis, Peter W. Mello, Sueli Corrêa Marques de |
dc.subject.por.fl_str_mv |
Feijão Sementes Controle biológico |
topic |
Feijão Sementes Controle biológico |
description |
Biological control of seed-borne pathogens has shown to enhance germination and physiological quality of seeds. The objectives of this study were to evaluate the in vitro antagonistic effect of five Trichoderma harzianum isolates (CEN287, CEN288, CEN289, CEN290, and CEN316) against Fusarium oxysporum f. sp. phaseoli (Foxy) and test its potential use in seed treatment. Initially, pathogen and antagonists were grown in paired cultures at 25ºC, from which samples were assessed using scanning electron microscopy (SEM). Then, clean or Foxy-infected seeds were treated with conidial suspension of the antagonists. Percent of Foxy-infected seeds and normal seedlings were evaluated at seven and nine days of incubation, respectively. All but one Trichoderma isolate (CEN290) inhibited Foxy mycelial growth. SEM analysis revealed that only one Trichoderma isolate (CEN287) showed parasitic interaction with Foxy. Two isolates (CEN287 and CEN316) significantly reduced the Foxy incidence and enhanced seed germination, though less effective than the fungicide mixture (carboxin + thiram). A principal component analysis indicated the importance of volatile metabolites in reducing Foxy incidence on common bean seeds. CEN287 Trichoderma harzianum isolate formed a single group due to its increase in germination rate of Foxyinfected seeds. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2017-12-07T05:09:13Z 2017-12-07T05:09:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CARVALHO, Daniel D. C. et al. Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment. Tropical Plant Pathology, Brasília, v. 39, n. 5, p. 384-391, out. 2014. DOI: http://dx.doi.org/10.1590/S1982-56762014000500005. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000500005&lng=en&nrm=iso. Acesso em: 30 out. 2019. http://repositorio.unb.br/handle/10482/29441 https://dx.doi.org/10.1590/S1982-56762014000500005 |
identifier_str_mv |
CARVALHO, Daniel D. C. et al. Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment. Tropical Plant Pathology, Brasília, v. 39, n. 5, p. 384-391, out. 2014. DOI: http://dx.doi.org/10.1590/S1982-56762014000500005. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000500005&lng=en&nrm=iso. Acesso em: 30 out. 2019. |
url |
http://repositorio.unb.br/handle/10482/29441 https://dx.doi.org/10.1590/S1982-56762014000500005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
instname_str |
Universidade de Brasília (UnB) |
instacron_str |
UNB |
institution |
UNB |
reponame_str |
Repositório Institucional da UnB |
collection |
Repositório Institucional da UnB |
repository.name.fl_str_mv |
Repositório Institucional da UnB - Universidade de Brasília (UnB) |
repository.mail.fl_str_mv |
repositorio@unb.br |
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