Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment

Detalhes bibliográficos
Autor(a) principal: Carvalho, Daniel Diego Costa
Data de Publicação: 2014
Outros Autores: Lobo Junior, Murillo, Martins, Irene, Inglis, Peter W., Mello, Sueli Corrêa Marques de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/29441
https://dx.doi.org/10.1590/S1982-56762014000500005
Resumo: Biological control of seed-borne pathogens has shown to enhance germination and physiological quality of seeds. The objectives of this study were to evaluate the in vitro antagonistic effect of five Trichoderma harzianum isolates (CEN287, CEN288, CEN289, CEN290, and CEN316) against Fusarium oxysporum f. sp. phaseoli (Foxy) and test its potential use in seed treatment. Initially, pathogen and antagonists were grown in paired cultures at 25ºC, from which samples were assessed using scanning electron microscopy (SEM). Then, clean or Foxy-infected seeds were treated with conidial suspension of the antagonists. Percent of Foxy-infected seeds and normal seedlings were evaluated at seven and nine days of incubation, respectively. All but one Trichoderma isolate (CEN290) inhibited Foxy mycelial growth. SEM analysis revealed that only one Trichoderma isolate (CEN287) showed parasitic interaction with Foxy. Two isolates (CEN287 and CEN316) significantly reduced the Foxy incidence and enhanced seed germination, though less effective than the fungicide mixture (carboxin + thiram). A principal component analysis indicated the importance of volatile metabolites in reducing Foxy incidence on common bean seeds. CEN287 Trichoderma harzianum isolate formed a single group due to its increase in germination rate of Foxyinfected seeds.
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spelling Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatmentFeijãoSementesControle biológicoBiological control of seed-borne pathogens has shown to enhance germination and physiological quality of seeds. The objectives of this study were to evaluate the in vitro antagonistic effect of five Trichoderma harzianum isolates (CEN287, CEN288, CEN289, CEN290, and CEN316) against Fusarium oxysporum f. sp. phaseoli (Foxy) and test its potential use in seed treatment. Initially, pathogen and antagonists were grown in paired cultures at 25ºC, from which samples were assessed using scanning electron microscopy (SEM). Then, clean or Foxy-infected seeds were treated with conidial suspension of the antagonists. Percent of Foxy-infected seeds and normal seedlings were evaluated at seven and nine days of incubation, respectively. All but one Trichoderma isolate (CEN290) inhibited Foxy mycelial growth. SEM analysis revealed that only one Trichoderma isolate (CEN287) showed parasitic interaction with Foxy. Two isolates (CEN287 and CEN316) significantly reduced the Foxy incidence and enhanced seed germination, though less effective than the fungicide mixture (carboxin + thiram). A principal component analysis indicated the importance of volatile metabolites in reducing Foxy incidence on common bean seeds. CEN287 Trichoderma harzianum isolate formed a single group due to its increase in germination rate of Foxyinfected seeds.Sociedade Brasileira de Fitopatologia2017-12-07T05:09:13Z2017-12-07T05:09:13Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCARVALHO, Daniel D. C. et al. Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment. Tropical Plant Pathology, Brasília, v. 39, n. 5, p. 384-391, out. 2014. DOI: http://dx.doi.org/10.1590/S1982-56762014000500005. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000500005&lng=en&nrm=iso. Acesso em: 30 out. 2019.http://repositorio.unb.br/handle/10482/29441https://dx.doi.org/10.1590/S1982-56762014000500005Tropical Plant Pathology - (CC BY NC) - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000500005&lng=en&nrm=iso&tlng=. Acesso em: 30 out. 2019.info:eu-repo/semantics/openAccessCarvalho, Daniel Diego CostaLobo Junior, MurilloMartins, IreneInglis, Peter W.Mello, Sueli Corrêa Marques deengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-05-24T23:45:10Zoai:repositorio.unb.br:10482/29441Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:45:10Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment
title Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment
spellingShingle Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment
Carvalho, Daniel Diego Costa
Feijão
Sementes
Controle biológico
title_short Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment
title_full Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment
title_fullStr Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment
title_full_unstemmed Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment
title_sort Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment
author Carvalho, Daniel Diego Costa
author_facet Carvalho, Daniel Diego Costa
Lobo Junior, Murillo
Martins, Irene
Inglis, Peter W.
Mello, Sueli Corrêa Marques de
author_role author
author2 Lobo Junior, Murillo
Martins, Irene
Inglis, Peter W.
Mello, Sueli Corrêa Marques de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Daniel Diego Costa
Lobo Junior, Murillo
Martins, Irene
Inglis, Peter W.
Mello, Sueli Corrêa Marques de
dc.subject.por.fl_str_mv Feijão
Sementes
Controle biológico
topic Feijão
Sementes
Controle biológico
description Biological control of seed-borne pathogens has shown to enhance germination and physiological quality of seeds. The objectives of this study were to evaluate the in vitro antagonistic effect of five Trichoderma harzianum isolates (CEN287, CEN288, CEN289, CEN290, and CEN316) against Fusarium oxysporum f. sp. phaseoli (Foxy) and test its potential use in seed treatment. Initially, pathogen and antagonists were grown in paired cultures at 25ºC, from which samples were assessed using scanning electron microscopy (SEM). Then, clean or Foxy-infected seeds were treated with conidial suspension of the antagonists. Percent of Foxy-infected seeds and normal seedlings were evaluated at seven and nine days of incubation, respectively. All but one Trichoderma isolate (CEN290) inhibited Foxy mycelial growth. SEM analysis revealed that only one Trichoderma isolate (CEN287) showed parasitic interaction with Foxy. Two isolates (CEN287 and CEN316) significantly reduced the Foxy incidence and enhanced seed germination, though less effective than the fungicide mixture (carboxin + thiram). A principal component analysis indicated the importance of volatile metabolites in reducing Foxy incidence on common bean seeds. CEN287 Trichoderma harzianum isolate formed a single group due to its increase in germination rate of Foxyinfected seeds.
publishDate 2014
dc.date.none.fl_str_mv 2014
2017-12-07T05:09:13Z
2017-12-07T05:09:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv CARVALHO, Daniel D. C. et al. Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment. Tropical Plant Pathology, Brasília, v. 39, n. 5, p. 384-391, out. 2014. DOI: http://dx.doi.org/10.1590/S1982-56762014000500005. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000500005&lng=en&nrm=iso. Acesso em: 30 out. 2019.
http://repositorio.unb.br/handle/10482/29441
https://dx.doi.org/10.1590/S1982-56762014000500005
identifier_str_mv CARVALHO, Daniel D. C. et al. Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment. Tropical Plant Pathology, Brasília, v. 39, n. 5, p. 384-391, out. 2014. DOI: http://dx.doi.org/10.1590/S1982-56762014000500005. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000500005&lng=en&nrm=iso. Acesso em: 30 out. 2019.
url http://repositorio.unb.br/handle/10482/29441
https://dx.doi.org/10.1590/S1982-56762014000500005
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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