Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio2.unb.br/jspui/handle/10482/46911 https://doi.org/10.1039/D0RA03240A https://orcid.org/0000-0003-3248-1286 https://orcid.org/0000-0002-2144-0959 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0003-1210-8329 |
Resumo: | We describe here a green method for the preparation of silver nanoparticles (AgNPs), by a microwave assisted synthesis route using Handroanthus impetiginosus underbark extract, with antibacterial activity. After optimizing the synthesis parameters with a Box–Benhken designed experiment, samples were characterized by powder XRD, TEM, UV-Vis spectroscopy, FTIR and zetametry. Using the overall optimized conditions of synthesis – time of reaction 15 min at 200 C and plant extract/AgNO3 volume ratio equal to 10% – highly crystalline 13.4 nm-sized spherical AgNPs in a well-dispersed colloidal state were obtained. It was also proved that the plant extract compounds act as reductant and capping agents during synthesis to functionalize AgNPs, resulting in a negatively charged surface with high values of zeta potential in a wide range of pH, from acidic to alkaline media. Biological activity tests against Staphylococcus aureus and Escherichia coli and cell viability experiments showed that synthesized AgNPs were not toxic to HaCaT mammalian cells and presented a high efficiency against Gram-positive bacteria (S. aureus). This was associated with the synergistic combination of AgNP silver cores with the capping layer containing natural compounds with antimicrobial properties and considered an alternative to the AgNPs commonly obtained from conventional routes that present antibacterial effectiveness preferentially against Gram-negative strains. |
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Illanes Tormena, Renata PascoalRosa, Eliane VieiraMota, Bruna de Fatima OliveiraChaker, Juliano AlexandreFagg, Christopher WilliamFreire, Daniel OliveiraMartins, Paula MeloSilva, Izabel Cristina Rodrigues daSousa, Marcelo HenriqueUniversity of Brasilia, Faculty of Ceilandia, Green Nanotechnology GroupUniversity of Brasilia, Faculty of Ceilandia, Green Nanotechnology GroupFederal Institute of Education, Science and Technology Goiano, Ceres, GOUniversity of Brasilia, Faculty of Ceilandia, Department of PharmacyUniversity of Brasilia, Faculty of Ceilandia, Green Nanotechnology GroupUniversity of Brasilia, Faculty of Ceilandia, Graduate Program in Health Sciences and TechnologiesLS Educational, Microbiology Laboratory, Brasilia, BrazilUniversity of Brasilia, Faculty of Ceilandia, Department of PharmacyUniversity of Brasilia, Faculty of Ceilandia, Graduate Program in Health Sciences and TechnologiesUniversity of Brasilia, Faculty of Ceilandia, Green Nanotechnology Group2023-11-27T13:46:52Z2023-11-27T13:46:52Z2020-06-21ILLANES TORMENA, Renata Pascoal et al. Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract. RSC Advances, [S. l.], v. 10, n. 35, 20676-20681, 2020. DOI: 10.1039/d0ra03240a. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA03240A. Acesso em: 28 nov. 2023.http://repositorio2.unb.br/jspui/handle/10482/46911https://doi.org/10.1039/D0RA03240Ahttps://orcid.org/0000-0003-3248-1286https://orcid.org/0000-0002-2144-0959https://orcid.org/0000-0001-6072-7832https://orcid.org/0000-0003-1210-8329engThe Royal Society of Chemistry(CC BY-NC). This article is licensed under aCreative Commons Attribution-NonCommercial 3.0 Unported Licenceinfo:eu-repo/semantics/openAccessEvaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extractinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAtividade antimicrobianaNanopartículas de prataIpê roxoWe describe here a green method for the preparation of silver nanoparticles (AgNPs), by a microwave assisted synthesis route using Handroanthus impetiginosus underbark extract, with antibacterial activity. After optimizing the synthesis parameters with a Box–Benhken designed experiment, samples were characterized by powder XRD, TEM, UV-Vis spectroscopy, FTIR and zetametry. Using the overall optimized conditions of synthesis – time of reaction 15 min at 200 C and plant extract/AgNO3 volume ratio equal to 10% – highly crystalline 13.4 nm-sized spherical AgNPs in a well-dispersed colloidal state were obtained. It was also proved that the plant extract compounds act as reductant and capping agents during synthesis to functionalize AgNPs, resulting in a negatively charged surface with high values of zeta potential in a wide range of pH, from acidic to alkaline media. Biological activity tests against Staphylococcus aureus and Escherichia coli and cell viability experiments showed that synthesized AgNPs were not toxic to HaCaT mammalian cells and presented a high efficiency against Gram-positive bacteria (S. aureus). This was associated with the synergistic combination of AgNP silver cores with the capping layer containing natural compounds with antimicrobial properties and considered an alternative to the AgNPs commonly obtained from conventional routes that present antibacterial effectiveness preferentially against Gram-negative strains.Faculdade UnB Ceilândia (FCE)Curso de Farmácia (FCE-FAR)Programa de Pós-Graduação em Ciências e Tecnologias em Saúdereponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_Evaluation of the antimicrobial activity.pdfARTIGO_Evaluation of the antimicrobial activity.pdfapplication/pdf639440http://repositorio2.unb.br/jspui/bitstream/10482/46911/3/ARTIGO_Evaluation%20of%20the%20antimicrobial%20activity.pdf696ad265c860778bf42a72120ee2ab5cMD53open accessLICENSElicense.txtlicense.txttext/plain102http://repositorio2.unb.br/jspui/bitstream/10482/46911/2/license.txtaed4704d04bb260d4decd80db311aaa5MD52open access10482/469112023-12-05 18:22:02.382open accessoai:repositorio2.unb.br:10482/46911U3VibWlzc8OjbyBlZmV0aXZhZGEgZGUgYWNvcmRvIGNvbSBsaWNlbsOnYSBjb25jZWRpZGEgcGVsbyBhdXRvciBlL291IGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcy4KBiblioteca Digital de Teses e DissertaçõesPUBhttps://repositorio.unb.br/oai/requestopendoar:2023-12-05T21:22:02Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.pt_BR.fl_str_mv |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
title |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
spellingShingle |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract Illanes Tormena, Renata Pascoal Atividade antimicrobiana Nanopartículas de prata Ipê roxo |
title_short |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
title_full |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
title_fullStr |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
title_full_unstemmed |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
title_sort |
Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
author |
Illanes Tormena, Renata Pascoal |
author_facet |
Illanes Tormena, Renata Pascoal Rosa, Eliane Vieira Mota, Bruna de Fatima Oliveira Chaker, Juliano Alexandre Fagg, Christopher William Freire, Daniel Oliveira Martins, Paula Melo Silva, Izabel Cristina Rodrigues da Sousa, Marcelo Henrique |
author_role |
author |
author2 |
Rosa, Eliane Vieira Mota, Bruna de Fatima Oliveira Chaker, Juliano Alexandre Fagg, Christopher William Freire, Daniel Oliveira Martins, Paula Melo Silva, Izabel Cristina Rodrigues da Sousa, Marcelo Henrique |
author2_role |
author author author author author author author author |
dc.contributor.affiliation.pt_BR.fl_str_mv |
University of Brasilia, Faculty of Ceilandia, Green Nanotechnology Group University of Brasilia, Faculty of Ceilandia, Green Nanotechnology Group Federal Institute of Education, Science and Technology Goiano, Ceres, GO University of Brasilia, Faculty of Ceilandia, Department of Pharmacy University of Brasilia, Faculty of Ceilandia, Green Nanotechnology Group University of Brasilia, Faculty of Ceilandia, Graduate Program in Health Sciences and Technologies LS Educational, Microbiology Laboratory, Brasilia, Brazil University of Brasilia, Faculty of Ceilandia, Department of Pharmacy University of Brasilia, Faculty of Ceilandia, Graduate Program in Health Sciences and Technologies University of Brasilia, Faculty of Ceilandia, Green Nanotechnology Group |
dc.contributor.author.fl_str_mv |
Illanes Tormena, Renata Pascoal Rosa, Eliane Vieira Mota, Bruna de Fatima Oliveira Chaker, Juliano Alexandre Fagg, Christopher William Freire, Daniel Oliveira Martins, Paula Melo Silva, Izabel Cristina Rodrigues da Sousa, Marcelo Henrique |
dc.subject.keyword.pt_BR.fl_str_mv |
Atividade antimicrobiana Nanopartículas de prata Ipê roxo |
topic |
Atividade antimicrobiana Nanopartículas de prata Ipê roxo |
description |
We describe here a green method for the preparation of silver nanoparticles (AgNPs), by a microwave assisted synthesis route using Handroanthus impetiginosus underbark extract, with antibacterial activity. After optimizing the synthesis parameters with a Box–Benhken designed experiment, samples were characterized by powder XRD, TEM, UV-Vis spectroscopy, FTIR and zetametry. Using the overall optimized conditions of synthesis – time of reaction 15 min at 200 C and plant extract/AgNO3 volume ratio equal to 10% – highly crystalline 13.4 nm-sized spherical AgNPs in a well-dispersed colloidal state were obtained. It was also proved that the plant extract compounds act as reductant and capping agents during synthesis to functionalize AgNPs, resulting in a negatively charged surface with high values of zeta potential in a wide range of pH, from acidic to alkaline media. Biological activity tests against Staphylococcus aureus and Escherichia coli and cell viability experiments showed that synthesized AgNPs were not toxic to HaCaT mammalian cells and presented a high efficiency against Gram-positive bacteria (S. aureus). This was associated with the synergistic combination of AgNP silver cores with the capping layer containing natural compounds with antimicrobial properties and considered an alternative to the AgNPs commonly obtained from conventional routes that present antibacterial effectiveness preferentially against Gram-negative strains. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-06-21 |
dc.date.accessioned.fl_str_mv |
2023-11-27T13:46:52Z |
dc.date.available.fl_str_mv |
2023-11-27T13:46:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ILLANES TORMENA, Renata Pascoal et al. Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract. RSC Advances, [S. l.], v. 10, n. 35, 20676-20681, 2020. DOI: 10.1039/d0ra03240a. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA03240A. Acesso em: 28 nov. 2023. |
dc.identifier.uri.fl_str_mv |
http://repositorio2.unb.br/jspui/handle/10482/46911 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1039/D0RA03240A |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0003-3248-1286 https://orcid.org/0000-0002-2144-0959 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0003-1210-8329 |
identifier_str_mv |
ILLANES TORMENA, Renata Pascoal et al. Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract. RSC Advances, [S. l.], v. 10, n. 35, 20676-20681, 2020. DOI: 10.1039/d0ra03240a. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA03240A. Acesso em: 28 nov. 2023. |
url |
http://repositorio2.unb.br/jspui/handle/10482/46911 https://doi.org/10.1039/D0RA03240A https://orcid.org/0000-0003-3248-1286 https://orcid.org/0000-0002-2144-0959 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0003-1210-8329 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
The Royal Society of Chemistry |
publisher.none.fl_str_mv |
The Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
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