Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region

Detalhes bibliográficos
Autor(a) principal: Rabello, Aline Rodrigues
Data de Publicação: 2008
Outros Autores: Queiroz, Paulo Roberto, Simões, Kenya Carla Cardoso, Hiragi, Cássia de Oliveira, Lima, Luzia Helena C., Oliveira, Maria Regina V., Mehta, Angela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/27349
https://doi.org/10.1590/S1415-47572008000300029
Resumo: The Bemisia tabaci complex is formed by approximately 41 biotypes, two of which (B and BR) occur in Brazil. In this work we aimed at obtaining genetic markers to assess the genetic diversity of the different biotypes. In order to do that we analyzed Bemisia tabaci biotypes B, BR, Q and Cassava using molecular techniques including RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. The analyses revealed a high similarity between the individuals of the B and Q biotypes, which could be distinguished from the BR individuals. A phylogenetic tree based on ITS1 rDNA sequence was constructed. This is the first report of the ITS1 rDNA sequence of Bemisia tuberculata and of the BR biotype of B. tabaci.
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spelling Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA regionMosca-brancaDiversidade genéticaAnálise filogenéticaThe Bemisia tabaci complex is formed by approximately 41 biotypes, two of which (B and BR) occur in Brazil. In this work we aimed at obtaining genetic markers to assess the genetic diversity of the different biotypes. In order to do that we analyzed Bemisia tabaci biotypes B, BR, Q and Cassava using molecular techniques including RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. The analyses revealed a high similarity between the individuals of the B and Q biotypes, which could be distinguished from the BR individuals. A phylogenetic tree based on ITS1 rDNA sequence was constructed. This is the first report of the ITS1 rDNA sequence of Bemisia tuberculata and of the BR biotype of B. tabaci.Faculdade de Agronomia e Medicina Veterinária (FAV)Sociedade Brasileira de Genética2017-12-07T04:50:15Z2017-12-07T04:50:15Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRABELLO, Aline R. et al. Diversity analysis of Bemisia tabaci biotypes: RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. Genetics and Molecular Biology, v. 31, n. 2, p. 585-590, 2008. DOI: https://doi.org/10.1590/S1415-47572008000300029. Disponível em: https://www.scielo.br/j/gmb/a/yGKfxdZC3qmRMMBCyZWsChP/abstract/?lang=en#. Acesso em: 20 maio 2022.http://repositorio.unb.br/handle/10482/27349https://doi.org/10.1590/S1415-47572008000300029This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.info:eu-repo/semantics/openAccessRabello, Aline RodriguesQueiroz, Paulo RobertoSimões, Kenya Carla CardosoHiragi, Cássia de OliveiraLima, Luzia Helena C.Oliveira, Maria Regina V.Mehta, Angelaengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-08-30T17:37:19Zoai:repositorio.unb.br:10482/27349Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-08-30T17:37:19Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
title Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
spellingShingle Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
Rabello, Aline Rodrigues
Mosca-branca
Diversidade genética
Análise filogenética
title_short Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
title_full Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
title_fullStr Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
title_full_unstemmed Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
title_sort Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
author Rabello, Aline Rodrigues
author_facet Rabello, Aline Rodrigues
Queiroz, Paulo Roberto
Simões, Kenya Carla Cardoso
Hiragi, Cássia de Oliveira
Lima, Luzia Helena C.
Oliveira, Maria Regina V.
Mehta, Angela
author_role author
author2 Queiroz, Paulo Roberto
Simões, Kenya Carla Cardoso
Hiragi, Cássia de Oliveira
Lima, Luzia Helena C.
Oliveira, Maria Regina V.
Mehta, Angela
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rabello, Aline Rodrigues
Queiroz, Paulo Roberto
Simões, Kenya Carla Cardoso
Hiragi, Cássia de Oliveira
Lima, Luzia Helena C.
Oliveira, Maria Regina V.
Mehta, Angela
dc.subject.por.fl_str_mv Mosca-branca
Diversidade genética
Análise filogenética
topic Mosca-branca
Diversidade genética
Análise filogenética
description The Bemisia tabaci complex is formed by approximately 41 biotypes, two of which (B and BR) occur in Brazil. In this work we aimed at obtaining genetic markers to assess the genetic diversity of the different biotypes. In order to do that we analyzed Bemisia tabaci biotypes B, BR, Q and Cassava using molecular techniques including RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. The analyses revealed a high similarity between the individuals of the B and Q biotypes, which could be distinguished from the BR individuals. A phylogenetic tree based on ITS1 rDNA sequence was constructed. This is the first report of the ITS1 rDNA sequence of Bemisia tuberculata and of the BR biotype of B. tabaci.
publishDate 2008
dc.date.none.fl_str_mv 2008
2017-12-07T04:50:15Z
2017-12-07T04:50:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv RABELLO, Aline R. et al. Diversity analysis of Bemisia tabaci biotypes: RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. Genetics and Molecular Biology, v. 31, n. 2, p. 585-590, 2008. DOI: https://doi.org/10.1590/S1415-47572008000300029. Disponível em: https://www.scielo.br/j/gmb/a/yGKfxdZC3qmRMMBCyZWsChP/abstract/?lang=en#. Acesso em: 20 maio 2022.
http://repositorio.unb.br/handle/10482/27349
https://doi.org/10.1590/S1415-47572008000300029
identifier_str_mv RABELLO, Aline R. et al. Diversity analysis of Bemisia tabaci biotypes: RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. Genetics and Molecular Biology, v. 31, n. 2, p. 585-590, 2008. DOI: https://doi.org/10.1590/S1415-47572008000300029. Disponível em: https://www.scielo.br/j/gmb/a/yGKfxdZC3qmRMMBCyZWsChP/abstract/?lang=en#. Acesso em: 20 maio 2022.
url http://repositorio.unb.br/handle/10482/27349
https://doi.org/10.1590/S1415-47572008000300029
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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