Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil

Detalhes bibliográficos
Autor(a) principal: Tavares, Talissa de Moraes
Data de Publicação: 2008
Outros Autores: Brito, Wilia Marta Elsner Diederichsen de, Fiaccadori, Fabíola Souza, Freitas, Erika Regina Leal de, Parente, Juliana Alves, Costa, Paulo Sérgio Sucasas da, Giugliano, Loreny Gimenes, Andreasi, Márcia Sueli Assis, Soares, Célia Maria Almeida, Cardoso, Divina das Dôres de Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/27170
https://dx.doi.org/10.1590/S0074-02762008000300011
Resumo: Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development.
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spelling Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of BrazilRotavírus grupo AGene nsp4GenótiposRegião Centro-OesteBrasilNonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development.Em processamentoInstituto Oswaldo Cruz, Ministério da Saúde2017-12-07T04:49:23Z2017-12-07T04:49:23Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMem. Inst. Oswaldo Cruz,v.103,n.3,p.288-294,2008http://repositorio.unb.br/handle/10482/27170https://dx.doi.org/10.1590/S0074-02762008000300011Tavares, Talissa de MoraesBrito, Wilia Marta Elsner Diederichsen deFiaccadori, Fabíola SouzaFreitas, Erika Regina Leal deParente, Juliana AlvesCosta, Paulo Sérgio Sucasas daGiugliano, Loreny GimenesAndreasi, Márcia Sueli AssisSoares, Célia Maria AlmeidaCardoso, Divina das Dôres de Paulainfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T19:04:09Zoai:repositorio.unb.br:10482/27170Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:09Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil
title Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil
spellingShingle Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil
Tavares, Talissa de Moraes
Rotavírus grupo A
Gene nsp4
Genótipos
Região Centro-Oeste
Brasil
title_short Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil
title_full Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil
title_fullStr Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil
title_full_unstemmed Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil
title_sort Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil
author Tavares, Talissa de Moraes
author_facet Tavares, Talissa de Moraes
Brito, Wilia Marta Elsner Diederichsen de
Fiaccadori, Fabíola Souza
Freitas, Erika Regina Leal de
Parente, Juliana Alves
Costa, Paulo Sérgio Sucasas da
Giugliano, Loreny Gimenes
Andreasi, Márcia Sueli Assis
Soares, Célia Maria Almeida
Cardoso, Divina das Dôres de Paula
author_role author
author2 Brito, Wilia Marta Elsner Diederichsen de
Fiaccadori, Fabíola Souza
Freitas, Erika Regina Leal de
Parente, Juliana Alves
Costa, Paulo Sérgio Sucasas da
Giugliano, Loreny Gimenes
Andreasi, Márcia Sueli Assis
Soares, Célia Maria Almeida
Cardoso, Divina das Dôres de Paula
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tavares, Talissa de Moraes
Brito, Wilia Marta Elsner Diederichsen de
Fiaccadori, Fabíola Souza
Freitas, Erika Regina Leal de
Parente, Juliana Alves
Costa, Paulo Sérgio Sucasas da
Giugliano, Loreny Gimenes
Andreasi, Márcia Sueli Assis
Soares, Célia Maria Almeida
Cardoso, Divina das Dôres de Paula
dc.subject.por.fl_str_mv Rotavírus grupo A
Gene nsp4
Genótipos
Região Centro-Oeste
Brasil
topic Rotavírus grupo A
Gene nsp4
Genótipos
Região Centro-Oeste
Brasil
description Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development.
publishDate 2008
dc.date.none.fl_str_mv 2008
2017-12-07T04:49:23Z
2017-12-07T04:49:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Mem. Inst. Oswaldo Cruz,v.103,n.3,p.288-294,2008
http://repositorio.unb.br/handle/10482/27170
https://dx.doi.org/10.1590/S0074-02762008000300011
identifier_str_mv Mem. Inst. Oswaldo Cruz,v.103,n.3,p.288-294,2008
url http://repositorio.unb.br/handle/10482/27170
https://dx.doi.org/10.1590/S0074-02762008000300011
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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