Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio.unb.br/handle/10482/27170 https://dx.doi.org/10.1590/S0074-02762008000300011 |
Resumo: | Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development. |
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Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of BrazilRotavírus grupo AGene nsp4GenótiposRegião Centro-OesteBrasilNonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development.Em processamentoInstituto Oswaldo Cruz, Ministério da Saúde2017-12-07T04:49:23Z2017-12-07T04:49:23Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMem. Inst. Oswaldo Cruz,v.103,n.3,p.288-294,2008http://repositorio.unb.br/handle/10482/27170https://dx.doi.org/10.1590/S0074-02762008000300011Tavares, Talissa de MoraesBrito, Wilia Marta Elsner Diederichsen deFiaccadori, Fabíola SouzaFreitas, Erika Regina Leal deParente, Juliana AlvesCosta, Paulo Sérgio Sucasas daGiugliano, Loreny GimenesAndreasi, Márcia Sueli AssisSoares, Célia Maria AlmeidaCardoso, Divina das Dôres de Paulainfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T19:04:09Zoai:repositorio.unb.br:10482/27170Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:09Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.none.fl_str_mv |
Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
title |
Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
spellingShingle |
Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil Tavares, Talissa de Moraes Rotavírus grupo A Gene nsp4 Genótipos Região Centro-Oeste Brasil |
title_short |
Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
title_full |
Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
title_fullStr |
Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
title_full_unstemmed |
Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
title_sort |
Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
author |
Tavares, Talissa de Moraes |
author_facet |
Tavares, Talissa de Moraes Brito, Wilia Marta Elsner Diederichsen de Fiaccadori, Fabíola Souza Freitas, Erika Regina Leal de Parente, Juliana Alves Costa, Paulo Sérgio Sucasas da Giugliano, Loreny Gimenes Andreasi, Márcia Sueli Assis Soares, Célia Maria Almeida Cardoso, Divina das Dôres de Paula |
author_role |
author |
author2 |
Brito, Wilia Marta Elsner Diederichsen de Fiaccadori, Fabíola Souza Freitas, Erika Regina Leal de Parente, Juliana Alves Costa, Paulo Sérgio Sucasas da Giugliano, Loreny Gimenes Andreasi, Márcia Sueli Assis Soares, Célia Maria Almeida Cardoso, Divina das Dôres de Paula |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Tavares, Talissa de Moraes Brito, Wilia Marta Elsner Diederichsen de Fiaccadori, Fabíola Souza Freitas, Erika Regina Leal de Parente, Juliana Alves Costa, Paulo Sérgio Sucasas da Giugliano, Loreny Gimenes Andreasi, Márcia Sueli Assis Soares, Célia Maria Almeida Cardoso, Divina das Dôres de Paula |
dc.subject.por.fl_str_mv |
Rotavírus grupo A Gene nsp4 Genótipos Região Centro-Oeste Brasil |
topic |
Rotavírus grupo A Gene nsp4 Genótipos Região Centro-Oeste Brasil |
description |
Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2017-12-07T04:49:23Z 2017-12-07T04:49:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Mem. Inst. Oswaldo Cruz,v.103,n.3,p.288-294,2008 http://repositorio.unb.br/handle/10482/27170 https://dx.doi.org/10.1590/S0074-02762008000300011 |
identifier_str_mv |
Mem. Inst. Oswaldo Cruz,v.103,n.3,p.288-294,2008 |
url |
http://repositorio.unb.br/handle/10482/27170 https://dx.doi.org/10.1590/S0074-02762008000300011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
instname_str |
Universidade de Brasília (UnB) |
instacron_str |
UNB |
institution |
UNB |
reponame_str |
Repositório Institucional da UnB |
collection |
Repositório Institucional da UnB |
repository.name.fl_str_mv |
Repositório Institucional da UnB - Universidade de Brasília (UnB) |
repository.mail.fl_str_mv |
repositorio@unb.br |
_version_ |
1814508399360999424 |