Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection

Detalhes bibliográficos
Autor(a) principal: Arruda, Mauro Maciel de
Data de Publicação: 2016
Outros Autores: Figueiredo, Fabiano Borges, Marcelino, Andreza Pain, Barbosa, José Ronaldo, Werneck, Guilherme Loureiro, Noronha, Elza Ferreira, Romero, Gustavo Adolfo Sierra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/30037
http://dx.doi.org/10.1590/0074-02760150364
Resumo: In Brazil, human and canine visceral leishmaniasis (CVL) caused byLeishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays (IFA) as the screening and confirmatory methods, respectively, for the detection of canine infection. The purpose of this study was to estimate the accuracy of ELISA and IFA in parallel or serial combinations. The reference standard comprised the results of direct visualisation of parasites in histological sections, immunohistochemical test, or isolation of the parasite in culture. Samples from 98 cases and 1,327 noncases were included. Individually, both tests presented sensitivity of 91.8% and 90.8%, and specificity of 83.4 and 53.4%, for the ELISA and IFA, respectively. When tests were used in parallel combination, sensitivity attained 99.2%, while specificity dropped to 44.8%. When used in serial combination (ELISA followed by IFA), decreased sensitivity (83.3%) and increased specificity (92.5%) were observed. Serial testing approach improved specificity with moderate loss in sensitivity. This strategy could partially fulfill the needs of public health and dog owners for a more accurate diagnosis of CVL.
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spelling Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infectionCãoProtozoárioLeishmaniose visceralSorologiaIn Brazil, human and canine visceral leishmaniasis (CVL) caused byLeishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays (IFA) as the screening and confirmatory methods, respectively, for the detection of canine infection. The purpose of this study was to estimate the accuracy of ELISA and IFA in parallel or serial combinations. The reference standard comprised the results of direct visualisation of parasites in histological sections, immunohistochemical test, or isolation of the parasite in culture. Samples from 98 cases and 1,327 noncases were included. Individually, both tests presented sensitivity of 91.8% and 90.8%, and specificity of 83.4 and 53.4%, for the ELISA and IFA, respectively. When tests were used in parallel combination, sensitivity attained 99.2%, while specificity dropped to 44.8%. When used in serial combination (ELISA followed by IFA), decreased sensitivity (83.3%) and increased specificity (92.5%) were observed. Serial testing approach improved specificity with moderate loss in sensitivity. This strategy could partially fulfill the needs of public health and dog owners for a more accurate diagnosis of CVL.Faculdade de Medicina (FMD)Instituto Oswaldo Cruz, Ministério da Saúde2017-12-07T05:17:09Z2017-12-07T05:17:09Z2016-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfARRUDA, Mauro Maciel de et al. Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 3, p. 168-173, mar. 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000300168&lng=en&nrm=iso>. Acesso em: 14 mar. 2018. Epub Feb 19, 2016. doi: http://dx.doi.org/10.1590/0074-02760150364.http://repositorio.unb.br/handle/10482/30037http://dx.doi.org/10.1590/0074-02760150364Memórias do Instituto Oswaldo Cruz - This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000300168&lng=en&nrm=iso. Acesso em: 14 mar. 2018.info:eu-repo/semantics/openAccessArruda, Mauro Maciel deFigueiredo, Fabiano BorgesMarcelino, Andreza PainBarbosa, José RonaldoWerneck, Guilherme LoureiroNoronha, Elza FerreiraRomero, Gustavo Adolfo Sierraengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-08-25T19:46:36Zoai:repositorio.unb.br:10482/30037Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-08-25T19:46:36Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
title Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
spellingShingle Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
Arruda, Mauro Maciel de
Cão
Protozoário
Leishmaniose visceral
Sorologia
title_short Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
title_full Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
title_fullStr Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
title_full_unstemmed Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
title_sort Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
author Arruda, Mauro Maciel de
author_facet Arruda, Mauro Maciel de
Figueiredo, Fabiano Borges
Marcelino, Andreza Pain
Barbosa, José Ronaldo
Werneck, Guilherme Loureiro
Noronha, Elza Ferreira
Romero, Gustavo Adolfo Sierra
author_role author
author2 Figueiredo, Fabiano Borges
Marcelino, Andreza Pain
Barbosa, José Ronaldo
Werneck, Guilherme Loureiro
Noronha, Elza Ferreira
Romero, Gustavo Adolfo Sierra
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Arruda, Mauro Maciel de
Figueiredo, Fabiano Borges
Marcelino, Andreza Pain
Barbosa, José Ronaldo
Werneck, Guilherme Loureiro
Noronha, Elza Ferreira
Romero, Gustavo Adolfo Sierra
dc.subject.por.fl_str_mv Cão
Protozoário
Leishmaniose visceral
Sorologia
topic Cão
Protozoário
Leishmaniose visceral
Sorologia
description In Brazil, human and canine visceral leishmaniasis (CVL) caused byLeishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays (IFA) as the screening and confirmatory methods, respectively, for the detection of canine infection. The purpose of this study was to estimate the accuracy of ELISA and IFA in parallel or serial combinations. The reference standard comprised the results of direct visualisation of parasites in histological sections, immunohistochemical test, or isolation of the parasite in culture. Samples from 98 cases and 1,327 noncases were included. Individually, both tests presented sensitivity of 91.8% and 90.8%, and specificity of 83.4 and 53.4%, for the ELISA and IFA, respectively. When tests were used in parallel combination, sensitivity attained 99.2%, while specificity dropped to 44.8%. When used in serial combination (ELISA followed by IFA), decreased sensitivity (83.3%) and increased specificity (92.5%) were observed. Serial testing approach improved specificity with moderate loss in sensitivity. This strategy could partially fulfill the needs of public health and dog owners for a more accurate diagnosis of CVL.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
2017-12-07T05:17:09Z
2017-12-07T05:17:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ARRUDA, Mauro Maciel de et al. Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 3, p. 168-173, mar. 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000300168&lng=en&nrm=iso>. Acesso em: 14 mar. 2018. Epub Feb 19, 2016. doi: http://dx.doi.org/10.1590/0074-02760150364.
http://repositorio.unb.br/handle/10482/30037
http://dx.doi.org/10.1590/0074-02760150364
identifier_str_mv ARRUDA, Mauro Maciel de et al. Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 3, p. 168-173, mar. 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000300168&lng=en&nrm=iso>. Acesso em: 14 mar. 2018. Epub Feb 19, 2016. doi: http://dx.doi.org/10.1590/0074-02760150364.
url http://repositorio.unb.br/handle/10482/30037
http://dx.doi.org/10.1590/0074-02760150364
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
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institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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