Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil

Detalhes bibliográficos
Autor(a) principal: Ampuero Vela, Julia Sonia
Data de Publicação: 2009
Outros Autores: Rios, Alexandre Pereira, Carranza Tamayo, César Omar, Romero, Gustavo Adolfo Sierra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/27434
https://dx.doi.org/10.1590/S0074-02762009000700009
Resumo: The positivities of two methods for the diagnosis of localised cutaneous leishmaniasis (CL) were estimated in 280 patients enrolled in a clinical trial. The trial was conducted in an endemic area of Leishmania (Viannia) braziliensis and trial participants were patients with skin ulcers and positive leishmanin skin tests. Patients underwent aspirative skin punctures of the ulcerated lesions and lymph nodes for in vitro cultures, which were processed under field conditions at the local health centre. Skin lesion biopsies were tested at a reference laboratory using kinetoplastid DNA (kDNA)-PCR to detect DNA. The median time required to obtain a positive culture from the skin samples was seven days and the contamination rate of the samples was 1.8%. The positivities of the cultures from skin lesions, kDNA-PCR and the combination of the two methods were 78.2% (95% CI: 73-82.6%), 89.3% (95% CI: 85.1-92.4%) and 97.1% (95% CI: 94.5-98.5%). We conclude that parasite culture is a feasible method for the detection of Leishmania in field conditions and that the combination of culture and PCR has a potential role for the diagnosis of CL in candidates for clinical trials.
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spelling Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in BrazilLeishmaniose cutâneaLeishmania braziliensisReação em cadeia de polimeraseEnsaios clínicosThe positivities of two methods for the diagnosis of localised cutaneous leishmaniasis (CL) were estimated in 280 patients enrolled in a clinical trial. The trial was conducted in an endemic area of Leishmania (Viannia) braziliensis and trial participants were patients with skin ulcers and positive leishmanin skin tests. Patients underwent aspirative skin punctures of the ulcerated lesions and lymph nodes for in vitro cultures, which were processed under field conditions at the local health centre. Skin lesion biopsies were tested at a reference laboratory using kinetoplastid DNA (kDNA)-PCR to detect DNA. The median time required to obtain a positive culture from the skin samples was seven days and the contamination rate of the samples was 1.8%. The positivities of the cultures from skin lesions, kDNA-PCR and the combination of the two methods were 78.2% (95% CI: 73-82.6%), 89.3% (95% CI: 85.1-92.4%) and 97.1% (95% CI: 94.5-98.5%). We conclude that parasite culture is a feasible method for the detection of Leishmania in field conditions and that the combination of culture and PCR has a potential role for the diagnosis of CL in candidates for clinical trials.Faculdade de Medicina (FMD)Instituto Oswaldo Cruz, Ministério da Saúde2017-12-07T04:51:48Z2017-12-07T04:51:48Z2009-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAMPUERO, Julia et al. Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 7, p. 992-997, nov. 2009. DOI: https://doi.org/10.1590/S0074-02762009000700009. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700009&lng=en&nrm=iso. Acesso em: 04 dez. 2020.http://repositorio.unb.br/handle/10482/27434https://dx.doi.org/10.1590/S0074-02762009000700009Memórias do Instituto Oswaldo Cruz - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700009&lng=en&tlng=en. Acesso em: 04 dez. 2020.info:eu-repo/semantics/openAccessAmpuero Vela, Julia SoniaRios, Alexandre PereiraCarranza Tamayo, César OmarRomero, Gustavo Adolfo Sierraengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-08-25T02:54:37Zoai:repositorio.unb.br:10482/27434Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-08-25T02:54:37Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil
title Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil
spellingShingle Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil
Ampuero Vela, Julia Sonia
Leishmaniose cutânea
Leishmania braziliensis
Reação em cadeia de polimerase
Ensaios clínicos
title_short Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil
title_full Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil
title_fullStr Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil
title_full_unstemmed Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil
title_sort Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil
author Ampuero Vela, Julia Sonia
author_facet Ampuero Vela, Julia Sonia
Rios, Alexandre Pereira
Carranza Tamayo, César Omar
Romero, Gustavo Adolfo Sierra
author_role author
author2 Rios, Alexandre Pereira
Carranza Tamayo, César Omar
Romero, Gustavo Adolfo Sierra
author2_role author
author
author
dc.contributor.author.fl_str_mv Ampuero Vela, Julia Sonia
Rios, Alexandre Pereira
Carranza Tamayo, César Omar
Romero, Gustavo Adolfo Sierra
dc.subject.por.fl_str_mv Leishmaniose cutânea
Leishmania braziliensis
Reação em cadeia de polimerase
Ensaios clínicos
topic Leishmaniose cutânea
Leishmania braziliensis
Reação em cadeia de polimerase
Ensaios clínicos
description The positivities of two methods for the diagnosis of localised cutaneous leishmaniasis (CL) were estimated in 280 patients enrolled in a clinical trial. The trial was conducted in an endemic area of Leishmania (Viannia) braziliensis and trial participants were patients with skin ulcers and positive leishmanin skin tests. Patients underwent aspirative skin punctures of the ulcerated lesions and lymph nodes for in vitro cultures, which were processed under field conditions at the local health centre. Skin lesion biopsies were tested at a reference laboratory using kinetoplastid DNA (kDNA)-PCR to detect DNA. The median time required to obtain a positive culture from the skin samples was seven days and the contamination rate of the samples was 1.8%. The positivities of the cultures from skin lesions, kDNA-PCR and the combination of the two methods were 78.2% (95% CI: 73-82.6%), 89.3% (95% CI: 85.1-92.4%) and 97.1% (95% CI: 94.5-98.5%). We conclude that parasite culture is a feasible method for the detection of Leishmania in field conditions and that the combination of culture and PCR has a potential role for the diagnosis of CL in candidates for clinical trials.
publishDate 2009
dc.date.none.fl_str_mv 2009-11
2017-12-07T04:51:48Z
2017-12-07T04:51:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv AMPUERO, Julia et al. Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 7, p. 992-997, nov. 2009. DOI: https://doi.org/10.1590/S0074-02762009000700009. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700009&lng=en&nrm=iso. Acesso em: 04 dez. 2020.
http://repositorio.unb.br/handle/10482/27434
https://dx.doi.org/10.1590/S0074-02762009000700009
identifier_str_mv AMPUERO, Julia et al. Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 7, p. 992-997, nov. 2009. DOI: https://doi.org/10.1590/S0074-02762009000700009. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000700009&lng=en&nrm=iso. Acesso em: 04 dez. 2020.
url http://repositorio.unb.br/handle/10482/27434
https://dx.doi.org/10.1590/S0074-02762009000700009
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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