Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface

Detalhes bibliográficos
Autor(a) principal: Maranhão, Andrea Queiroz
Data de Publicação: 2000
Outros Autores: Brígido, Marcelo de Macedo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/25608
https://dx.doi.org/10.1590/S0100-879X2000000500012
Resumo: We describe the expression of an anti-Z-DNA single chain variable region antibody fragment (scFv) on a filamentous phage surface. Four vectors for phage display were constructed. Two of them are able to display multiple copies of the antibody fragment, and the others can be used to make monovalent libraries. The vectors use different promoter/leader sequences to direct the expression of the fused proteins. All were able to promote the assembly of fusion virion particles. In this paper we also show the affinity selection (biopanning) of those phage-antibodies based on the capacity of their products to recognize the antigen. We used biotinylated Z-DNA and the selection was performed in a solution phase fashion. The data presented here indicate that these vectors can be further used to construct anti-nucleic acid antibody fragment libraries that can be used to study the basis of nucleic acid-protein interaction and its role in autoimmunity mechanisms.
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spelling Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surfaceAnticorpos antiácido nucléicoZ-DNAWe describe the expression of an anti-Z-DNA single chain variable region antibody fragment (scFv) on a filamentous phage surface. Four vectors for phage display were constructed. Two of them are able to display multiple copies of the antibody fragment, and the others can be used to make monovalent libraries. The vectors use different promoter/leader sequences to direct the expression of the fused proteins. All were able to promote the assembly of fusion virion particles. In this paper we also show the affinity selection (biopanning) of those phage-antibodies based on the capacity of their products to recognize the antigen. We used biotinylated Z-DNA and the selection was performed in a solution phase fashion. The data presented here indicate that these vectors can be further used to construct anti-nucleic acid antibody fragment libraries that can be used to study the basis of nucleic acid-protein interaction and its role in autoimmunity mechanisms.Em processamentoAssociação Brasileira de Divulgação Científica2017-12-07T04:32:53Z2017-12-07T04:32:53Z2000info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBraz J Med Biol Res,v.33,n.5,p.569-579,2000http://repositorio.unb.br/handle/10482/25608https://dx.doi.org/10.1590/S0100-879X2000000500012Maranhão, Andrea QueirozBrígido, Marcelo de Macedoinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T19:04:08Zoai:repositorio.unb.br:10482/25608Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:08Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
title Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
spellingShingle Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
Maranhão, Andrea Queiroz
Anticorpos antiácido nucléico
Z-DNA
title_short Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
title_full Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
title_fullStr Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
title_full_unstemmed Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
title_sort Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
author Maranhão, Andrea Queiroz
author_facet Maranhão, Andrea Queiroz
Brígido, Marcelo de Macedo
author_role author
author2 Brígido, Marcelo de Macedo
author2_role author
dc.contributor.author.fl_str_mv Maranhão, Andrea Queiroz
Brígido, Marcelo de Macedo
dc.subject.por.fl_str_mv Anticorpos antiácido nucléico
Z-DNA
topic Anticorpos antiácido nucléico
Z-DNA
description We describe the expression of an anti-Z-DNA single chain variable region antibody fragment (scFv) on a filamentous phage surface. Four vectors for phage display were constructed. Two of them are able to display multiple copies of the antibody fragment, and the others can be used to make monovalent libraries. The vectors use different promoter/leader sequences to direct the expression of the fused proteins. All were able to promote the assembly of fusion virion particles. In this paper we also show the affinity selection (biopanning) of those phage-antibodies based on the capacity of their products to recognize the antigen. We used biotinylated Z-DNA and the selection was performed in a solution phase fashion. The data presented here indicate that these vectors can be further used to construct anti-nucleic acid antibody fragment libraries that can be used to study the basis of nucleic acid-protein interaction and its role in autoimmunity mechanisms.
publishDate 2000
dc.date.none.fl_str_mv 2000
2017-12-07T04:32:53Z
2017-12-07T04:32:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Braz J Med Biol Res,v.33,n.5,p.569-579,2000
http://repositorio.unb.br/handle/10482/25608
https://dx.doi.org/10.1590/S0100-879X2000000500012
identifier_str_mv Braz J Med Biol Res,v.33,n.5,p.569-579,2000
url http://repositorio.unb.br/handle/10482/25608
https://dx.doi.org/10.1590/S0100-879X2000000500012
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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