Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice

Detalhes bibliográficos
Autor(a) principal: Souza, Danielle Galdino de
Data de Publicação: 2022
Outros Autores: Santos, Débora Silva, Simon, Karina Smidt, Morais, José Athayde Vasconcelos, Coelho, Luísa Coutinho, Pacheco, Thyago José Arruda, Azevedo, Ricardo Bentes, Bocca, Anamélia Lorenzetti, Melo-Silva, César Augusto, Longo, João Paulo Figueiró
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio2.unb.br/jspui/handle/10482/46042
https://doi.org/10.3390/nano12101683
https://orcid.org/0000-0003-3432-0769
https://orcid.org/0000-0003-2395-7748
https://orcid.org/0000-0002-8090-0644
https://orcid.org/0000-0002-2137-9588
https://orcid.org/0000-0003-3323-5300
https://orcid.org/0000-0002-3544-6999
Resumo: Diets rich in omega-3 or -6 fatty acids will produce different profiles for cell membranes phospholipid constitutions. Omegas 3 and 6 are part of the diet and can modulate the inflammatory profile. We evaluated the effects of the oral absorption of fish oil, when associated with a lipid nanoemulsion in an experimental pulmonary inflammatory model. Pulmonary fibrosis is a disease associated with excessive extracellular matrix deposition. We determined to investigate the morphophysiological mechanisms in mice that were pretreated after induction with bleomycin (BLM). The pretreatment was for 21 days with saline solution, sunflower oil (SO), fish oil (FO), and fish oil nanoemulsion (NEW3). The animals received a daily dose of 50 mg/Kg of docosahexaenoic acid DHA and 10 mg/Kg eicosapentaenoic (EPA) (100 mg/Kg), represented by a daily dose of 40 µL of NEW3. The blank group was treated with the same amount daily (40 µL) during the 21 days of pretreatment. The animals were treated with SO and FO, 100 mg/Kg (containing 58 mg/Kg of polyunsaturated fats/higher% linoleic acid) and 100 mg/Kg (50 mg/Kg of DHA and 10 mg/Kg EPA), respectively. A single dose of 5 mg/mL (50 µL) bleomycin sulfate, by the intratracheal surgical method in BALB/cAnNTac (BALB/c). NEW3 significantly reduced fibrotic progression, which can be evidenced by the protection from loss of body mass, increase in respiratory incursions per minute, decreased spacing of alveolar septa, decreased severity of fibrosis, and changes in the respiratory system. NEW3 attenuated the inflammatory changes developed in the experimental model of pulmonary fibrosis, while group SO showed a significant increase in inflammatory changes. This concluded that the presented results demonstrated that is possible to positively modulate the immune and inflamamtory response to an external agressor, by changing the nutitional intake of specific fatty acids, such as omega-3 placed in fish oil. Moreover, these benefits can be improved by the nanoencapsulation of fish oil in lipid nanoemulsions.
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spelling Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c MiceNanoemulsãoFibrose pulmonarMedicamentosÓleos de peixeDiets rich in omega-3 or -6 fatty acids will produce different profiles for cell membranes phospholipid constitutions. Omegas 3 and 6 are part of the diet and can modulate the inflammatory profile. We evaluated the effects of the oral absorption of fish oil, when associated with a lipid nanoemulsion in an experimental pulmonary inflammatory model. Pulmonary fibrosis is a disease associated with excessive extracellular matrix deposition. We determined to investigate the morphophysiological mechanisms in mice that were pretreated after induction with bleomycin (BLM). The pretreatment was for 21 days with saline solution, sunflower oil (SO), fish oil (FO), and fish oil nanoemulsion (NEW3). The animals received a daily dose of 50 mg/Kg of docosahexaenoic acid DHA and 10 mg/Kg eicosapentaenoic (EPA) (100 mg/Kg), represented by a daily dose of 40 µL of NEW3. The blank group was treated with the same amount daily (40 µL) during the 21 days of pretreatment. The animals were treated with SO and FO, 100 mg/Kg (containing 58 mg/Kg of polyunsaturated fats/higher% linoleic acid) and 100 mg/Kg (50 mg/Kg of DHA and 10 mg/Kg EPA), respectively. A single dose of 5 mg/mL (50 µL) bleomycin sulfate, by the intratracheal surgical method in BALB/cAnNTac (BALB/c). NEW3 significantly reduced fibrotic progression, which can be evidenced by the protection from loss of body mass, increase in respiratory incursions per minute, decreased spacing of alveolar septa, decreased severity of fibrosis, and changes in the respiratory system. NEW3 attenuated the inflammatory changes developed in the experimental model of pulmonary fibrosis, while group SO showed a significant increase in inflammatory changes. This concluded that the presented results demonstrated that is possible to positively modulate the immune and inflamamtory response to an external agressor, by changing the nutitional intake of specific fatty acids, such as omega-3 placed in fish oil. Moreover, these benefits can be improved by the nanoencapsulation of fish oil in lipid nanoemulsions.Instituto de Ciências Biológicas (IB)Departamento de Genética e Morfologia (IB GEM)Departamento de Biologia Celular (IB CEL)Faculdade de Medicina (FMD)Programa de Pós-Graduação em Nanociência e NanobiotecnologiaMDPIUniversity of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology DepartmentUniversity of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology DepartmentUniversity of Brasília, Institute of Biological Science, Applied Immunology Laboratory, Cell Biology DepartmentUniversity of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology DepartmentUniversity of Brasília, Institute of Biological Science, Applied Immunology Laboratory, Cell Biology DepartmentUniversity of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology DepartmentUniversity of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology DepartmentUniversity of Brasília, Institute of Biological Science, Applied Immunology Laboratory, Cell Biology DepartmentUniversity of Brasília, Faculty of Medicine, Respiratory Physiology LaboratoryUniversity of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology DepartmentSouza, Danielle Galdino deSantos, Débora SilvaSimon, Karina SmidtMorais, José Athayde VasconcelosCoelho, Luísa CoutinhoPacheco, Thyago José ArrudaAzevedo, Ricardo BentesBocca, Anamélia LorenzettiMelo-Silva, César AugustoLongo, João Paulo Figueiró2023-07-10T14:29:53Z2023-07-10T14:29:53Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSOUZA, Danielle Galdino de Souza; SANTOS, Débora Silva Santos; SIMON, Karina Smidt Simon; MORAIS, José Athayde Vasconcelos COELHO, Luísa Coutinho Coelho; PACHECO, Thyago José Arruda; AZEVEDO, Ricardo Bentes; BOCCA, Anamélia Lorenzetti; MELO-SILVA, César Augusto Melo-Silva; LONGO, João Paulo Figueiró. Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice. Nanomaterials, [S.l.], v. 12, 1683, 2022. DOI: https://doi.org/10.3390/nano12101683. Disponível em: https://www.mdpi.com/2079-4991/12/10/1683. Acesso em: 09 jul. 2023.http://repositorio2.unb.br/jspui/handle/10482/46042https://doi.org/10.3390/nano12101683https://orcid.org/0000-0003-3432-0769https://orcid.org/0000-0003-2395-7748https://orcid.org/0000-0002-8090-0644https://orcid.org/0000-0002-2137-9588https://orcid.org/0000-0003-3323-5300https://orcid.org/0000-0002-3544-6999eng© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-10-05T15:05:43Zoai:repositorio.unb.br:10482/46042Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-10-05T15:05:43Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice
title Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice
spellingShingle Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice
Souza, Danielle Galdino de
Nanoemulsão
Fibrose pulmonar
Medicamentos
Óleos de peixe
title_short Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice
title_full Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice
title_fullStr Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice
title_full_unstemmed Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice
title_sort Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice
author Souza, Danielle Galdino de
author_facet Souza, Danielle Galdino de
Santos, Débora Silva
Simon, Karina Smidt
Morais, José Athayde Vasconcelos
Coelho, Luísa Coutinho
Pacheco, Thyago José Arruda
Azevedo, Ricardo Bentes
Bocca, Anamélia Lorenzetti
Melo-Silva, César Augusto
Longo, João Paulo Figueiró
author_role author
author2 Santos, Débora Silva
Simon, Karina Smidt
Morais, José Athayde Vasconcelos
Coelho, Luísa Coutinho
Pacheco, Thyago José Arruda
Azevedo, Ricardo Bentes
Bocca, Anamélia Lorenzetti
Melo-Silva, César Augusto
Longo, João Paulo Figueiró
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department
University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department
University of Brasília, Institute of Biological Science, Applied Immunology Laboratory, Cell Biology Department
University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department
University of Brasília, Institute of Biological Science, Applied Immunology Laboratory, Cell Biology Department
University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department
University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department
University of Brasília, Institute of Biological Science, Applied Immunology Laboratory, Cell Biology Department
University of Brasília, Faculty of Medicine, Respiratory Physiology Laboratory
University of Brasília, Institute of Biological Science, Nanobiotechnology Laboratory, Genetics & Morphology Department
dc.contributor.author.fl_str_mv Souza, Danielle Galdino de
Santos, Débora Silva
Simon, Karina Smidt
Morais, José Athayde Vasconcelos
Coelho, Luísa Coutinho
Pacheco, Thyago José Arruda
Azevedo, Ricardo Bentes
Bocca, Anamélia Lorenzetti
Melo-Silva, César Augusto
Longo, João Paulo Figueiró
dc.subject.por.fl_str_mv Nanoemulsão
Fibrose pulmonar
Medicamentos
Óleos de peixe
topic Nanoemulsão
Fibrose pulmonar
Medicamentos
Óleos de peixe
description Diets rich in omega-3 or -6 fatty acids will produce different profiles for cell membranes phospholipid constitutions. Omegas 3 and 6 are part of the diet and can modulate the inflammatory profile. We evaluated the effects of the oral absorption of fish oil, when associated with a lipid nanoemulsion in an experimental pulmonary inflammatory model. Pulmonary fibrosis is a disease associated with excessive extracellular matrix deposition. We determined to investigate the morphophysiological mechanisms in mice that were pretreated after induction with bleomycin (BLM). The pretreatment was for 21 days with saline solution, sunflower oil (SO), fish oil (FO), and fish oil nanoemulsion (NEW3). The animals received a daily dose of 50 mg/Kg of docosahexaenoic acid DHA and 10 mg/Kg eicosapentaenoic (EPA) (100 mg/Kg), represented by a daily dose of 40 µL of NEW3. The blank group was treated with the same amount daily (40 µL) during the 21 days of pretreatment. The animals were treated with SO and FO, 100 mg/Kg (containing 58 mg/Kg of polyunsaturated fats/higher% linoleic acid) and 100 mg/Kg (50 mg/Kg of DHA and 10 mg/Kg EPA), respectively. A single dose of 5 mg/mL (50 µL) bleomycin sulfate, by the intratracheal surgical method in BALB/cAnNTac (BALB/c). NEW3 significantly reduced fibrotic progression, which can be evidenced by the protection from loss of body mass, increase in respiratory incursions per minute, decreased spacing of alveolar septa, decreased severity of fibrosis, and changes in the respiratory system. NEW3 attenuated the inflammatory changes developed in the experimental model of pulmonary fibrosis, while group SO showed a significant increase in inflammatory changes. This concluded that the presented results demonstrated that is possible to positively modulate the immune and inflamamtory response to an external agressor, by changing the nutitional intake of specific fatty acids, such as omega-3 placed in fish oil. Moreover, these benefits can be improved by the nanoencapsulation of fish oil in lipid nanoemulsions.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023-07-10T14:29:53Z
2023-07-10T14:29:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SOUZA, Danielle Galdino de Souza; SANTOS, Débora Silva Santos; SIMON, Karina Smidt Simon; MORAIS, José Athayde Vasconcelos COELHO, Luísa Coutinho Coelho; PACHECO, Thyago José Arruda; AZEVEDO, Ricardo Bentes; BOCCA, Anamélia Lorenzetti; MELO-SILVA, César Augusto Melo-Silva; LONGO, João Paulo Figueiró. Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice. Nanomaterials, [S.l.], v. 12, 1683, 2022. DOI: https://doi.org/10.3390/nano12101683. Disponível em: https://www.mdpi.com/2079-4991/12/10/1683. Acesso em: 09 jul. 2023.
http://repositorio2.unb.br/jspui/handle/10482/46042
https://doi.org/10.3390/nano12101683
https://orcid.org/0000-0003-3432-0769
https://orcid.org/0000-0003-2395-7748
https://orcid.org/0000-0002-8090-0644
https://orcid.org/0000-0002-2137-9588
https://orcid.org/0000-0003-3323-5300
https://orcid.org/0000-0002-3544-6999
identifier_str_mv SOUZA, Danielle Galdino de Souza; SANTOS, Débora Silva Santos; SIMON, Karina Smidt Simon; MORAIS, José Athayde Vasconcelos COELHO, Luísa Coutinho Coelho; PACHECO, Thyago José Arruda; AZEVEDO, Ricardo Bentes; BOCCA, Anamélia Lorenzetti; MELO-SILVA, César Augusto Melo-Silva; LONGO, João Paulo Figueiró. Fish oil nanoemulsion supplementation attenuates bleomycin-induced pulmonary fibrosis BALB/c Mice. Nanomaterials, [S.l.], v. 12, 1683, 2022. DOI: https://doi.org/10.3390/nano12101683. Disponível em: https://www.mdpi.com/2079-4991/12/10/1683. Acesso em: 09 jul. 2023.
url http://repositorio2.unb.br/jspui/handle/10482/46042
https://doi.org/10.3390/nano12101683
https://orcid.org/0000-0003-3432-0769
https://orcid.org/0000-0003-2395-7748
https://orcid.org/0000-0002-8090-0644
https://orcid.org/0000-0002-2137-9588
https://orcid.org/0000-0003-3323-5300
https://orcid.org/0000-0002-3544-6999
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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