Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) <A NAME="Home"></A>

Detalhes bibliográficos
Autor(a) principal: Aragão, T.C.F.R.
Data de Publicação: 2000
Outros Autores: Souza, P.A.S., Uchôa, A.F., Costa, I.R., Bloch Jr., C., Campos, F.A.P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/25609
https://dx.doi.org/10.1590/S0100-879X2000000800005
Resumo: We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.
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spelling Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) <A NAME="Home"></A>CactoMandacarúCereus jamacaruCactina2s AlbuminasProteína rica em metioninaWe describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.Em processamentoAssociação Brasileira de Divulgação Científica2017-12-07T04:32:53Z2017-12-07T04:32:53Z2000info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBraz J Med Biol Res,v.33,n.8,p.897-903,2000http://repositorio.unb.br/handle/10482/25609https://dx.doi.org/10.1590/S0100-879X2000000800005Aragão, T.C.F.R.Souza, P.A.S.Uchôa, A.F.Costa, I.R.Bloch Jr., C.Campos, F.A.P.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T19:04:07Zoai:repositorio.unb.br:10482/25609Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:07Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) <A NAME="Home"></A>
title Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) <A NAME="Home"></A>
spellingShingle Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) <A NAME="Home"></A>
Aragão, T.C.F.R.
Cacto
Mandacarú
Cereus jamacaru
Cactina
2s Albuminas
Proteína rica em metionina
title_short Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) <A NAME="Home"></A>
title_full Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) <A NAME="Home"></A>
title_fullStr Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) <A NAME="Home"></A>
title_full_unstemmed Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) <A NAME="Home"></A>
title_sort Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) <A NAME="Home"></A>
author Aragão, T.C.F.R.
author_facet Aragão, T.C.F.R.
Souza, P.A.S.
Uchôa, A.F.
Costa, I.R.
Bloch Jr., C.
Campos, F.A.P.
author_role author
author2 Souza, P.A.S.
Uchôa, A.F.
Costa, I.R.
Bloch Jr., C.
Campos, F.A.P.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Aragão, T.C.F.R.
Souza, P.A.S.
Uchôa, A.F.
Costa, I.R.
Bloch Jr., C.
Campos, F.A.P.
dc.subject.por.fl_str_mv Cacto
Mandacarú
Cereus jamacaru
Cactina
2s Albuminas
Proteína rica em metionina
topic Cacto
Mandacarú
Cereus jamacaru
Cactina
2s Albuminas
Proteína rica em metionina
description We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.
publishDate 2000
dc.date.none.fl_str_mv 2000
2017-12-07T04:32:53Z
2017-12-07T04:32:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Braz J Med Biol Res,v.33,n.8,p.897-903,2000
http://repositorio.unb.br/handle/10482/25609
https://dx.doi.org/10.1590/S0100-879X2000000800005
identifier_str_mv Braz J Med Biol Res,v.33,n.8,p.897-903,2000
url http://repositorio.unb.br/handle/10482/25609
https://dx.doi.org/10.1590/S0100-879X2000000800005
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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