Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidas
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Diversitas Journal |
Texto Completo: | https://diversitasjournal.com.br/diversitas_journal/article/view/1849 |
Resumo: | ABSTRACT: The objective of this research was analyze the susceptibility of Plutella xylostella (L.) (Lepidoptera: Plutellidae) to isolates of Bacillus thuringiensis Berliner and to evaluate the compatibility of the most virulent isolates with insecticides registered to control cabbage pests. The efficiency of 13 isolates was evaluated through pathogenicity and virulence tests (LC50 and LT50) on second instar larvae of P. xylostella. The compatibility between the isolates and active ingredients was performed by the insecticide mixture in culture medium, and after this was inoculated an aliquot of microorganism suspension, being analyzed the number of spores / mL after seven days of entomopathogen growth. The isolates HD-1, HD-4, HD-11, HD-73, and T-07 were the most efficient on P. xylostella, and responsible to cause total mortality of immature, with LC50 varying between 0.75 and 11.66 × 103 spores / mL, and LT50 between 25.12 and 34.47 h. The isolates HD-1 and HD-4 showed neutral interaction with the active ingredient thiamethoxam, and positive interaction with the isolate T-07, with significant increase of sporulation. The other active ingredients were toxic in relation to the reproductive development of B. thuringiensis. KEYWORDS: diamondback moth, entomopathogenic bacterium, biological control, chemical control. |
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Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidasABSTRACT: The objective of this research was analyze the susceptibility of Plutella xylostella (L.) (Lepidoptera: Plutellidae) to isolates of Bacillus thuringiensis Berliner and to evaluate the compatibility of the most virulent isolates with insecticides registered to control cabbage pests. The efficiency of 13 isolates was evaluated through pathogenicity and virulence tests (LC50 and LT50) on second instar larvae of P. xylostella. The compatibility between the isolates and active ingredients was performed by the insecticide mixture in culture medium, and after this was inoculated an aliquot of microorganism suspension, being analyzed the number of spores / mL after seven days of entomopathogen growth. The isolates HD-1, HD-4, HD-11, HD-73, and T-07 were the most efficient on P. xylostella, and responsible to cause total mortality of immature, with LC50 varying between 0.75 and 11.66 × 103 spores / mL, and LT50 between 25.12 and 34.47 h. The isolates HD-1 and HD-4 showed neutral interaction with the active ingredient thiamethoxam, and positive interaction with the isolate T-07, with significant increase of sporulation. The other active ingredients were toxic in relation to the reproductive development of B. thuringiensis. KEYWORDS: diamondback moth, entomopathogenic bacterium, biological control, chemical control.RESUMO: O objetivo da pesquisa foi analisar a suscetibilidade de Plutella xylostella (L.) (Lepidoptera: Plutellidae) a isolados de Bacillus thuringiensis Berliner e avaliar a compatibilidade dos isolados mais virulentos com inseticidas registrados para o controle de pragas do repolho. Foi avaliada a eficiência de 13 isolados, através de testes de patogenicidade e virulência (CL50 e TL50) em lagartas de segundo instar de P. xylostella. A compatibilidade entre os isolados e os ingredientes ativos foi realizada a partir da mistura do inseticida no meio de cultura, com posterior inoculação de uma alíquota da suspensão do micro-organismo, sendo avaliado o número de esporos / mL após sete dias de crescimento do entomopatógeno. Os isolados HD-1, HD-4, HD-11, HD-73 e T-07 foram os mais eficientes no controle de P. xylostella, responsáveis por ocasionar mortalidade total das lagartas, com CL50 variando entre 0,75 e 11,66 × 103 esporos / mL e TL50 entre 25,12 e 34,47 h. Os isolados HD-1 e HD-4 apresentaram interação neutra com o ingrediente ativo tiametoxam, sendo positiva para com o isolado T-07, com aumento significativo da esporulação. Os demais ingredientes ativos foram considerados tóxicos em relação ao desenvolvimento reprodutivo de B. thuringiensis. PALAVRAS-CHAVE: traça-das-crucíferas, bactéria entomopatogênica, controle biológico.Universidade Estadual de Alagoas - Eduneal2021-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://diversitasjournal.com.br/diversitas_journal/article/view/184910.48017/Diversitas_Journal-v6i3-1849Diversitas Journal; v. 6 n. 3 (2021): A cultura e o turismo de mãos dadas com o desenvolvimento territorial; 2969-29832525-521510.48017/Diversitas_Journal-v6i3reponame:Diversitas Journalinstname:Universidade Estadual de Alagoas (UNEAL)instacron:UNEALporhttps://diversitasjournal.com.br/diversitas_journal/article/view/1849/1441Copyright (c) 2021 Rogério Teixeira Duartehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDuarte, Rogério TeixeiraBortoli, Sergio Antonio dePolanczyk, Ricardo Antonio2021-08-20T02:07:45Zoai:ojs.emnuvens.com.br:article/1849Revistahttps://diversitasjournal.com.br/diversitas_journal/indexPUBhttps://www.e-publicacoes.uerj.br/index.php/muralinternacional/oairevistadiversitasjournal@gmail.com2525-52152525-5215opendoar:2023-01-13T09:47:23.566163Diversitas Journal - Universidade Estadual de Alagoas (UNEAL)false |
dc.title.none.fl_str_mv |
Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidas |
title |
Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidas |
spellingShingle |
Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidas Duarte, Rogério Teixeira |
title_short |
Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidas |
title_full |
Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidas |
title_fullStr |
Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidas |
title_full_unstemmed |
Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidas |
title_sort |
Eficiência de Bacillus thuringiensis no controle de Plutella xylostella (Lepidoptera: Plutellidae) e compatibilidade com inseticidas |
author |
Duarte, Rogério Teixeira |
author_facet |
Duarte, Rogério Teixeira Bortoli, Sergio Antonio de Polanczyk, Ricardo Antonio |
author_role |
author |
author2 |
Bortoli, Sergio Antonio de Polanczyk, Ricardo Antonio |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Duarte, Rogério Teixeira Bortoli, Sergio Antonio de Polanczyk, Ricardo Antonio |
description |
ABSTRACT: The objective of this research was analyze the susceptibility of Plutella xylostella (L.) (Lepidoptera: Plutellidae) to isolates of Bacillus thuringiensis Berliner and to evaluate the compatibility of the most virulent isolates with insecticides registered to control cabbage pests. The efficiency of 13 isolates was evaluated through pathogenicity and virulence tests (LC50 and LT50) on second instar larvae of P. xylostella. The compatibility between the isolates and active ingredients was performed by the insecticide mixture in culture medium, and after this was inoculated an aliquot of microorganism suspension, being analyzed the number of spores / mL after seven days of entomopathogen growth. The isolates HD-1, HD-4, HD-11, HD-73, and T-07 were the most efficient on P. xylostella, and responsible to cause total mortality of immature, with LC50 varying between 0.75 and 11.66 × 103 spores / mL, and LT50 between 25.12 and 34.47 h. The isolates HD-1 and HD-4 showed neutral interaction with the active ingredient thiamethoxam, and positive interaction with the isolate T-07, with significant increase of sporulation. The other active ingredients were toxic in relation to the reproductive development of B. thuringiensis. KEYWORDS: diamondback moth, entomopathogenic bacterium, biological control, chemical control. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://diversitasjournal.com.br/diversitas_journal/article/view/1849 10.48017/Diversitas_Journal-v6i3-1849 |
url |
https://diversitasjournal.com.br/diversitas_journal/article/view/1849 |
identifier_str_mv |
10.48017/Diversitas_Journal-v6i3-1849 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://diversitasjournal.com.br/diversitas_journal/article/view/1849/1441 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Rogério Teixeira Duarte https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Rogério Teixeira Duarte https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Alagoas - Eduneal |
publisher.none.fl_str_mv |
Universidade Estadual de Alagoas - Eduneal |
dc.source.none.fl_str_mv |
Diversitas Journal; v. 6 n. 3 (2021): A cultura e o turismo de mãos dadas com o desenvolvimento territorial; 2969-2983 2525-5215 10.48017/Diversitas_Journal-v6i3 reponame:Diversitas Journal instname:Universidade Estadual de Alagoas (UNEAL) instacron:UNEAL |
instname_str |
Universidade Estadual de Alagoas (UNEAL) |
instacron_str |
UNEAL |
institution |
UNEAL |
reponame_str |
Diversitas Journal |
collection |
Diversitas Journal |
repository.name.fl_str_mv |
Diversitas Journal - Universidade Estadual de Alagoas (UNEAL) |
repository.mail.fl_str_mv |
revistadiversitasjournal@gmail.com |
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1797051278681964544 |