Detection of Badnavirus in pineapple in northeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Diversitas Journal |
Texto Completo: | https://diversitasjournal.com.br/diversitas_journal/article/view/1284 |
Resumo: | ABSTRACT: Brazil is one of the main global producers of pineapple (Ananas comosus L. Merril), with emphasis in the Northeastern region of the country. Pineapple is exposed to several phytosanitary problems, including viruses. Pineapple badnaviruses are caused by two distinct species: Pineapple bacilliform CO virus (PBCoV) and Pineapple bacilliform ER virus (PBErV). The present study aimed to detect possible species of the genus Badnavirus in pineapple samples in Northeastern Brazil, via PCR and sequencing of the RT/RNaseH region. Leaf samples of pineapples were collected in the states of Alagoas, Maranhão, Paraiba and Pernambuco, and subsequently subjected to total DNA extraction and amplification via PCR. Badnavirus positive samples were selected for sequencing. Analysis of pairwise comparisons revealed that all sequences obtained in this work showed an identity greater than 80% with the sequence of the species PBCoV (EU377664), from Australia, fully corroborating with phylogenetic analyzes. These results suggest the widespread of PBCoV in Northeastern Brazil and record the first report of Badnavirus in pineapple culture in Brazil. KEYWORDS: Caulimoviridae, Ananas comosus, molecular analysis. |
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Detection of Badnavirus in pineapple in northeastern BrazilABSTRACT: Brazil is one of the main global producers of pineapple (Ananas comosus L. Merril), with emphasis in the Northeastern region of the country. Pineapple is exposed to several phytosanitary problems, including viruses. Pineapple badnaviruses are caused by two distinct species: Pineapple bacilliform CO virus (PBCoV) and Pineapple bacilliform ER virus (PBErV). The present study aimed to detect possible species of the genus Badnavirus in pineapple samples in Northeastern Brazil, via PCR and sequencing of the RT/RNaseH region. Leaf samples of pineapples were collected in the states of Alagoas, Maranhão, Paraiba and Pernambuco, and subsequently subjected to total DNA extraction and amplification via PCR. Badnavirus positive samples were selected for sequencing. Analysis of pairwise comparisons revealed that all sequences obtained in this work showed an identity greater than 80% with the sequence of the species PBCoV (EU377664), from Australia, fully corroborating with phylogenetic analyzes. These results suggest the widespread of PBCoV in Northeastern Brazil and record the first report of Badnavirus in pineapple culture in Brazil. KEYWORDS: Caulimoviridae, Ananas comosus, molecular analysis.RESUMO: O Brasil é um dos principais produtores mundiais de abacaxi (Ananas comosus L. Merril), sendo a região Nordeste destaque em âmbito nacional. O abacaxizeiro está exposto a diversos problemas fitossanitários, inclusive as viroses. As badnaviroses do abacaxizeiro são causadas por duas espécies distintas: Pineapple bacilliform CO virus (PBCoV) e Pineapple bacilliform ER virus (PBErV). O presente estudo teve como objetivo detectar possíveis espécies do gênero Badnavirus em amostras de abacaxizeiro no Nordeste do Brasil, via PCR e sequenciamento da região RT/RNaseH. Amostras foliares de abacaxizeiros foram coletadas nos estados de Alagoas, Maranhão, Paraíba e Pernambuco e, posteriormente, submetidas à extração de DNA total e amplificação via PCR. Amostras positivas para Badnavirus foram selecionadas para sequenciamento. Análises de comparações de sequências pareadas revelaram que todas as sequências obtidas neste trabalho apresentaram identidade superior a 80% com a sequência da espécie PBCoV (EU377664), proveniente da Austrália, corroborando integralmente com as análises filogenéticas. Estes resultados sugerem a ampla disseminação do PBCoV no Nordeste brasileiro e registra o primeiro relato de Badnavirus na cultura do abacaxizeiro no Brasil. KEYWORDS: Caulimoviridae, Ananas comosus, análise molecular.Universidade Estadual de Alagoas - Eduneal2020-10-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://diversitasjournal.com.br/diversitas_journal/article/view/128410.17648/diversitas-journal-v5i4-1284Diversitas Journal; v. 5 n. 4 (2020): Agricultura familiar: a base da economia latinoamericana; 2450-24632525-521510.17648/diversitas-journal-v5i4reponame:Diversitas Journalinstname:Universidade Estadual de Alagoas (UNEAL)instacron:UNEALporhttps://diversitasjournal.com.br/diversitas_journal/article/view/1284/1098Copyright (c) 2020 Edlene Maria da Silva Moraes Santos, Jaqueline Figueredo de Oliveira Costa, Mayra Machado de Medeiros Ferro, Sarah Jacqueline Cavalcanti da Silva, Iraildes Pereira Assunção, Gaus Silvestre de Andrade Limahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantos, Edlene Maria da Silva MoraesFigueredo de Oliveira Costa, JaquelineMachado de Medeiros Ferro, MayraSilva, Sarah Jacqueline Cavalcanti daAssunção, Iraildes PereiraLima, Gaus Silvestre de Andrade2021-08-19T18:09:38Zoai:ojs.emnuvens.com.br:article/1284Revistahttps://diversitasjournal.com.br/diversitas_journal/indexPUBhttps://www.e-publicacoes.uerj.br/index.php/muralinternacional/oairevistadiversitasjournal@gmail.com2525-52152525-5215opendoar:2023-01-13T09:47:11.336994Diversitas Journal - Universidade Estadual de Alagoas (UNEAL)false |
dc.title.none.fl_str_mv |
Detection of Badnavirus in pineapple in northeastern Brazil |
title |
Detection of Badnavirus in pineapple in northeastern Brazil |
spellingShingle |
Detection of Badnavirus in pineapple in northeastern Brazil Santos, Edlene Maria da Silva Moraes |
title_short |
Detection of Badnavirus in pineapple in northeastern Brazil |
title_full |
Detection of Badnavirus in pineapple in northeastern Brazil |
title_fullStr |
Detection of Badnavirus in pineapple in northeastern Brazil |
title_full_unstemmed |
Detection of Badnavirus in pineapple in northeastern Brazil |
title_sort |
Detection of Badnavirus in pineapple in northeastern Brazil |
author |
Santos, Edlene Maria da Silva Moraes |
author_facet |
Santos, Edlene Maria da Silva Moraes Figueredo de Oliveira Costa, Jaqueline Machado de Medeiros Ferro, Mayra Silva, Sarah Jacqueline Cavalcanti da Assunção, Iraildes Pereira Lima, Gaus Silvestre de Andrade |
author_role |
author |
author2 |
Figueredo de Oliveira Costa, Jaqueline Machado de Medeiros Ferro, Mayra Silva, Sarah Jacqueline Cavalcanti da Assunção, Iraildes Pereira Lima, Gaus Silvestre de Andrade |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Edlene Maria da Silva Moraes Figueredo de Oliveira Costa, Jaqueline Machado de Medeiros Ferro, Mayra Silva, Sarah Jacqueline Cavalcanti da Assunção, Iraildes Pereira Lima, Gaus Silvestre de Andrade |
description |
ABSTRACT: Brazil is one of the main global producers of pineapple (Ananas comosus L. Merril), with emphasis in the Northeastern region of the country. Pineapple is exposed to several phytosanitary problems, including viruses. Pineapple badnaviruses are caused by two distinct species: Pineapple bacilliform CO virus (PBCoV) and Pineapple bacilliform ER virus (PBErV). The present study aimed to detect possible species of the genus Badnavirus in pineapple samples in Northeastern Brazil, via PCR and sequencing of the RT/RNaseH region. Leaf samples of pineapples were collected in the states of Alagoas, Maranhão, Paraiba and Pernambuco, and subsequently subjected to total DNA extraction and amplification via PCR. Badnavirus positive samples were selected for sequencing. Analysis of pairwise comparisons revealed that all sequences obtained in this work showed an identity greater than 80% with the sequence of the species PBCoV (EU377664), from Australia, fully corroborating with phylogenetic analyzes. These results suggest the widespread of PBCoV in Northeastern Brazil and record the first report of Badnavirus in pineapple culture in Brazil. KEYWORDS: Caulimoviridae, Ananas comosus, molecular analysis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://diversitasjournal.com.br/diversitas_journal/article/view/1284 10.17648/diversitas-journal-v5i4-1284 |
url |
https://diversitasjournal.com.br/diversitas_journal/article/view/1284 |
identifier_str_mv |
10.17648/diversitas-journal-v5i4-1284 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://diversitasjournal.com.br/diversitas_journal/article/view/1284/1098 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Alagoas - Eduneal |
publisher.none.fl_str_mv |
Universidade Estadual de Alagoas - Eduneal |
dc.source.none.fl_str_mv |
Diversitas Journal; v. 5 n. 4 (2020): Agricultura familiar: a base da economia latinoamericana; 2450-2463 2525-5215 10.17648/diversitas-journal-v5i4 reponame:Diversitas Journal instname:Universidade Estadual de Alagoas (UNEAL) instacron:UNEAL |
instname_str |
Universidade Estadual de Alagoas (UNEAL) |
instacron_str |
UNEAL |
institution |
UNEAL |
reponame_str |
Diversitas Journal |
collection |
Diversitas Journal |
repository.name.fl_str_mv |
Diversitas Journal - Universidade Estadual de Alagoas (UNEAL) |
repository.mail.fl_str_mv |
revistadiversitasjournal@gmail.com |
_version_ |
1797051277232832512 |