Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.

Detalhes bibliográficos
Autor(a) principal: Ferreira da Silva, Walisson
Data de Publicação: 2023
Outros Autores: Câmara dos Santos, Jockeliny Mayara, Laurentino, Jackeline, da Silva França, Kevison Romulo, Figueredo de Oliveira Costa, Jaqueline, Silvetre de Andrade Lima, Gaus, Pereira Assunção, Iraildes
Tipo de documento: Artigo
Idioma: por
Título da fonte: Diversitas Journal
Texto Completo: https://diversitasjournal.com.br/diversitas_journal/article/view/2420
Resumo: Anthracnose, caused by Colletotrichum spp., is an important disease of atemoya causing damage both pre and post-harvest. Symptoms of this disease range from foliar anthracnose, flower abortion, branch blight and necrotic fruit lesions. Knowing the action of certain fungicides in the fight against the etiological agent is essential to develop control techniques. The objective of this work was to evaluate the mycelial sensitivity of Colletotrichum spp., from atemoya, to the fungicides thiophanate methyl, azoxystrobin and tebuconazole. To determine the sensitivity of Colletotrichum species to fungicides, mycelial growth in synthetic potato-dextrose-agar (PDA) supplemented with fungicide was evaluated. The fungicides were dissolved in dimethylsulfoxide (DMSO) and added to the fluxing culture medium (45°C), to reach concentrations of 0.0, 0.1, 0.5, 1.0, 5.0 and 10 μg/ml-1 of a.i. The mycelial growth of each colony was measured daily to obtain the mycelial growth index (ICM). EC50 was also calculated. After calculating the EC50, the Colletotrichum species were classified into three sensitivity categories, where: EC50: <10 μg/ml-1: high sensitivity (SA); EC50: 10-100 μg/ml-1: moderate sensitivity (MS); EC50: 100-500 μg/ml-1: insensitivity (I). The species C. theobromicola, C. fruticola, C. siamense and C. karstii were highly sensitive to the fungicides tebuconazole and thiophanate methyl. The fungicide azoxystrobin proved to be efficient in the in vitro control of the species C. theobromicola, however, it proved to be inefficient for the control of the species C. siamense. The species C. fruticola and C. karstii were moderately sensitive to the fungicide azoxystrobin.
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spelling Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.antracnose, anonáceas, controle químicoanthracnose, annonaceae, chemical controlAnthracnose, caused by Colletotrichum spp., is an important disease of atemoya causing damage both pre and post-harvest. Symptoms of this disease range from foliar anthracnose, flower abortion, branch blight and necrotic fruit lesions. Knowing the action of certain fungicides in the fight against the etiological agent is essential to develop control techniques. The objective of this work was to evaluate the mycelial sensitivity of Colletotrichum spp., from atemoya, to the fungicides thiophanate methyl, azoxystrobin and tebuconazole. To determine the sensitivity of Colletotrichum species to fungicides, mycelial growth in synthetic potato-dextrose-agar (PDA) supplemented with fungicide was evaluated. The fungicides were dissolved in dimethylsulfoxide (DMSO) and added to the fluxing culture medium (45°C), to reach concentrations of 0.0, 0.1, 0.5, 1.0, 5.0 and 10 μg/ml-1 of a.i. The mycelial growth of each colony was measured daily to obtain the mycelial growth index (ICM). EC50 was also calculated. After calculating the EC50, the Colletotrichum species were classified into three sensitivity categories, where: EC50: <10 μg/ml-1: high sensitivity (SA); EC50: 10-100 μg/ml-1: moderate sensitivity (MS); EC50: 100-500 μg/ml-1: insensitivity (I). The species C. theobromicola, C. fruticola, C. siamense and C. karstii were highly sensitive to the fungicides tebuconazole and thiophanate methyl. The fungicide azoxystrobin proved to be efficient in the in vitro control of the species C. theobromicola, however, it proved to be inefficient for the control of the species C. siamense. The species C. fruticola and C. karstii were moderately sensitive to the fungicide azoxystrobin. R E S U M O A antracnose, causada por Colletotrichum spp., é uma importante doença da atemoia causando danos tanto na pré como na pós-colheita. Os sintomas desta doença variam de antracnose foliar, abortamento de flores, queima de ramos e lesões necróticas nos frutos. Conhecer a ação de determinados fungicidas no combate ao agente etiológico é essencial para desenvolver técnicas de controle. O trabalho teve como objetivo avaliar a sensibilidade micelial de Colletotrichum spp., provenientes de atemoia, aos fungicidas tiofanato metílico, azoxistrobina e tebuconazole. Para determinar a sensibilidade das espécies de Colletotrichum aos fungicidas foi avaliado o crescimento micelial em meio de batata-dextrose-ágar (BDA) sintético suplementado com fungicida. Os fungicidas foram dissolvidos em dimetilsulfóxido (DMSO) e adicionados ao meio de cultura fundente (45°C), para alcançar as concentrações de 0.0, 0.1, 0.5, 1.0, 5.0 e 10 μg/ml-1 de i.a. O crescimento micelial de cada colônia foi mensurado diariamente para obter o índice de crescimento micelial (ICM). A EC50 também foi calculada. Após o cálculo da EC50, as espécies de Colletotrichum foram classificadas em três categorias de sensibilidade, onde: EC50: <10 μg/ml-1: alta sensibilidade (AS); EC50: 10-100 μg/ml-1: moderada sensibilidade (MS); EC50: 100-500 μg/ml-1: insensibilidade (I). As espécies C. theobromicola, C. fruticola, C. siamense e C. karstii foram altamente sensíveis aos fungicidas tebuconazole e tiofanato metílico. O fungicida azoxistrobina se mostrou eficiente no controle in vitro da espécie C. theobromicola, porém, se mostrou ineficiente para o controle da espécie C. siamense. As espécies C. fruticola e C. karstii foram moderadamente sensíveis ao fungicida azoxistrobina.   Universidade Estadual de Alagoas - Eduneal2023-01-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://diversitasjournal.com.br/diversitas_journal/article/view/242010.48017/dj.v8i1.2420Diversitas Journal; Vol. 8 No. 1 (2023): Sociedade e ciência: um diálogo necessárioDiversitas Journal; Vol. 8 Núm. 1 (2023): Sociedade e ciência: um diálogo necessárioDiversitas Journal; v. 8 n. 1 (2023): Sociedade e ciência: um diálogo necessário2525-521510.48017/dj.v8i1reponame:Diversitas Journalinstname:Universidade Estadual de Alagoas (UNEAL)instacron:UNEALporhttps://diversitasjournal.com.br/diversitas_journal/article/view/2420/1953https://diversitasjournal.com.br/diversitas_journal/article/view/2420/1954Copyright (c) 2022 Jaqueline Figueredo de Oliveira Costa, Walisson Ferreira da Silva, JOCKELINY MAYARA CÂMARA DOS SANTOS, Jackeline Laurentino, Kevison Romulo da Silva França, Gaus Silvetre de Andrade Lima, Iraildes Pereira Assunçãohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFerreira da Silva, Walisson Câmara dos Santos, Jockeliny MayaraLaurentino, Jackeline da Silva França, Kevison RomuloFigueredo de Oliveira Costa, JaquelineSilvetre de Andrade Lima, Gaus Pereira Assunção, Iraildes2023-02-14T20:21:21Zoai:ojs.diversitasjournal.com.br:article/2420Revistahttps://diversitasjournal.com.br/diversitas_journal/indexPUBhttps://www.e-publicacoes.uerj.br/index.php/muralinternacional/oairevistadiversitasjournal@gmail.com2525-52152525-5215opendoar:2023-02-14T20:21:21Diversitas Journal - Universidade Estadual de Alagoas (UNEAL)false
dc.title.none.fl_str_mv Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.
title Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.
spellingShingle Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.
Ferreira da Silva, Walisson
antracnose, anonáceas, controle químico
anthracnose, annonaceae, chemical control
title_short Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.
title_full Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.
title_fullStr Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.
title_full_unstemmed Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.
title_sort Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.
author Ferreira da Silva, Walisson
author_facet Ferreira da Silva, Walisson
Câmara dos Santos, Jockeliny Mayara
Laurentino, Jackeline
da Silva França, Kevison Romulo
Figueredo de Oliveira Costa, Jaqueline
Silvetre de Andrade Lima, Gaus
Pereira Assunção, Iraildes
author_role author
author2 Câmara dos Santos, Jockeliny Mayara
Laurentino, Jackeline
da Silva França, Kevison Romulo
Figueredo de Oliveira Costa, Jaqueline
Silvetre de Andrade Lima, Gaus
Pereira Assunção, Iraildes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira da Silva, Walisson
Câmara dos Santos, Jockeliny Mayara
Laurentino, Jackeline
da Silva França, Kevison Romulo
Figueredo de Oliveira Costa, Jaqueline
Silvetre de Andrade Lima, Gaus
Pereira Assunção, Iraildes
dc.subject.por.fl_str_mv antracnose, anonáceas, controle químico
anthracnose, annonaceae, chemical control
topic antracnose, anonáceas, controle químico
anthracnose, annonaceae, chemical control
description Anthracnose, caused by Colletotrichum spp., is an important disease of atemoya causing damage both pre and post-harvest. Symptoms of this disease range from foliar anthracnose, flower abortion, branch blight and necrotic fruit lesions. Knowing the action of certain fungicides in the fight against the etiological agent is essential to develop control techniques. The objective of this work was to evaluate the mycelial sensitivity of Colletotrichum spp., from atemoya, to the fungicides thiophanate methyl, azoxystrobin and tebuconazole. To determine the sensitivity of Colletotrichum species to fungicides, mycelial growth in synthetic potato-dextrose-agar (PDA) supplemented with fungicide was evaluated. The fungicides were dissolved in dimethylsulfoxide (DMSO) and added to the fluxing culture medium (45°C), to reach concentrations of 0.0, 0.1, 0.5, 1.0, 5.0 and 10 μg/ml-1 of a.i. The mycelial growth of each colony was measured daily to obtain the mycelial growth index (ICM). EC50 was also calculated. After calculating the EC50, the Colletotrichum species were classified into three sensitivity categories, where: EC50: <10 μg/ml-1: high sensitivity (SA); EC50: 10-100 μg/ml-1: moderate sensitivity (MS); EC50: 100-500 μg/ml-1: insensitivity (I). The species C. theobromicola, C. fruticola, C. siamense and C. karstii were highly sensitive to the fungicides tebuconazole and thiophanate methyl. The fungicide azoxystrobin proved to be efficient in the in vitro control of the species C. theobromicola, however, it proved to be inefficient for the control of the species C. siamense. The species C. fruticola and C. karstii were moderately sensitive to the fungicide azoxystrobin.
publishDate 2023
dc.date.none.fl_str_mv 2023-01-10
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dc.identifier.uri.fl_str_mv https://diversitasjournal.com.br/diversitas_journal/article/view/2420
10.48017/dj.v8i1.2420
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dc.language.iso.fl_str_mv por
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dc.relation.none.fl_str_mv https://diversitasjournal.com.br/diversitas_journal/article/view/2420/1953
https://diversitasjournal.com.br/diversitas_journal/article/view/2420/1954
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dc.publisher.none.fl_str_mv Universidade Estadual de Alagoas - Eduneal
publisher.none.fl_str_mv Universidade Estadual de Alagoas - Eduneal
dc.source.none.fl_str_mv Diversitas Journal; Vol. 8 No. 1 (2023): Sociedade e ciência: um diálogo necessário
Diversitas Journal; Vol. 8 Núm. 1 (2023): Sociedade e ciência: um diálogo necessário
Diversitas Journal; v. 8 n. 1 (2023): Sociedade e ciência: um diálogo necessário
2525-5215
10.48017/dj.v8i1
reponame:Diversitas Journal
instname:Universidade Estadual de Alagoas (UNEAL)
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