Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Diversitas Journal |
Texto Completo: | https://diversitasjournal.com.br/diversitas_journal/article/view/2420 |
Resumo: | Anthracnose, caused by Colletotrichum spp., is an important disease of atemoya causing damage both pre and post-harvest. Symptoms of this disease range from foliar anthracnose, flower abortion, branch blight and necrotic fruit lesions. Knowing the action of certain fungicides in the fight against the etiological agent is essential to develop control techniques. The objective of this work was to evaluate the mycelial sensitivity of Colletotrichum spp., from atemoya, to the fungicides thiophanate methyl, azoxystrobin and tebuconazole. To determine the sensitivity of Colletotrichum species to fungicides, mycelial growth in synthetic potato-dextrose-agar (PDA) supplemented with fungicide was evaluated. The fungicides were dissolved in dimethylsulfoxide (DMSO) and added to the fluxing culture medium (45°C), to reach concentrations of 0.0, 0.1, 0.5, 1.0, 5.0 and 10 μg/ml-1 of a.i. The mycelial growth of each colony was measured daily to obtain the mycelial growth index (ICM). EC50 was also calculated. After calculating the EC50, the Colletotrichum species were classified into three sensitivity categories, where: EC50: <10 μg/ml-1: high sensitivity (SA); EC50: 10-100 μg/ml-1: moderate sensitivity (MS); EC50: 100-500 μg/ml-1: insensitivity (I). The species C. theobromicola, C. fruticola, C. siamense and C. karstii were highly sensitive to the fungicides tebuconazole and thiophanate methyl. The fungicide azoxystrobin proved to be efficient in the in vitro control of the species C. theobromicola, however, it proved to be inefficient for the control of the species C. siamense. The species C. fruticola and C. karstii were moderately sensitive to the fungicide azoxystrobin. |
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Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas.antracnose, anonáceas, controle químicoanthracnose, annonaceae, chemical controlAnthracnose, caused by Colletotrichum spp., is an important disease of atemoya causing damage both pre and post-harvest. Symptoms of this disease range from foliar anthracnose, flower abortion, branch blight and necrotic fruit lesions. Knowing the action of certain fungicides in the fight against the etiological agent is essential to develop control techniques. The objective of this work was to evaluate the mycelial sensitivity of Colletotrichum spp., from atemoya, to the fungicides thiophanate methyl, azoxystrobin and tebuconazole. To determine the sensitivity of Colletotrichum species to fungicides, mycelial growth in synthetic potato-dextrose-agar (PDA) supplemented with fungicide was evaluated. The fungicides were dissolved in dimethylsulfoxide (DMSO) and added to the fluxing culture medium (45°C), to reach concentrations of 0.0, 0.1, 0.5, 1.0, 5.0 and 10 μg/ml-1 of a.i. The mycelial growth of each colony was measured daily to obtain the mycelial growth index (ICM). EC50 was also calculated. After calculating the EC50, the Colletotrichum species were classified into three sensitivity categories, where: EC50: <10 μg/ml-1: high sensitivity (SA); EC50: 10-100 μg/ml-1: moderate sensitivity (MS); EC50: 100-500 μg/ml-1: insensitivity (I). The species C. theobromicola, C. fruticola, C. siamense and C. karstii were highly sensitive to the fungicides tebuconazole and thiophanate methyl. The fungicide azoxystrobin proved to be efficient in the in vitro control of the species C. theobromicola, however, it proved to be inefficient for the control of the species C. siamense. The species C. fruticola and C. karstii were moderately sensitive to the fungicide azoxystrobin. R E S U M O A antracnose, causada por Colletotrichum spp., é uma importante doença da atemoia causando danos tanto na pré como na pós-colheita. Os sintomas desta doença variam de antracnose foliar, abortamento de flores, queima de ramos e lesões necróticas nos frutos. Conhecer a ação de determinados fungicidas no combate ao agente etiológico é essencial para desenvolver técnicas de controle. O trabalho teve como objetivo avaliar a sensibilidade micelial de Colletotrichum spp., provenientes de atemoia, aos fungicidas tiofanato metílico, azoxistrobina e tebuconazole. Para determinar a sensibilidade das espécies de Colletotrichum aos fungicidas foi avaliado o crescimento micelial em meio de batata-dextrose-ágar (BDA) sintético suplementado com fungicida. Os fungicidas foram dissolvidos em dimetilsulfóxido (DMSO) e adicionados ao meio de cultura fundente (45°C), para alcançar as concentrações de 0.0, 0.1, 0.5, 1.0, 5.0 e 10 μg/ml-1 de i.a. O crescimento micelial de cada colônia foi mensurado diariamente para obter o índice de crescimento micelial (ICM). A EC50 também foi calculada. Após o cálculo da EC50, as espécies de Colletotrichum foram classificadas em três categorias de sensibilidade, onde: EC50: <10 μg/ml-1: alta sensibilidade (AS); EC50: 10-100 μg/ml-1: moderada sensibilidade (MS); EC50: 100-500 μg/ml-1: insensibilidade (I). As espécies C. theobromicola, C. fruticola, C. siamense e C. karstii foram altamente sensíveis aos fungicidas tebuconazole e tiofanato metílico. O fungicida azoxistrobina se mostrou eficiente no controle in vitro da espécie C. theobromicola, porém, se mostrou ineficiente para o controle da espécie C. siamense. As espécies C. fruticola e C. karstii foram moderadamente sensíveis ao fungicida azoxistrobina. Universidade Estadual de Alagoas - Eduneal2023-01-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://diversitasjournal.com.br/diversitas_journal/article/view/242010.48017/dj.v8i1.2420Diversitas Journal; Vol. 8 No. 1 (2023): Sociedade e ciência: um diálogo necessárioDiversitas Journal; Vol. 8 Núm. 1 (2023): Sociedade e ciência: um diálogo necessárioDiversitas Journal; v. 8 n. 1 (2023): Sociedade e ciência: um diálogo necessário2525-521510.48017/dj.v8i1reponame:Diversitas Journalinstname:Universidade Estadual de Alagoas (UNEAL)instacron:UNEALporhttps://diversitasjournal.com.br/diversitas_journal/article/view/2420/1953https://diversitasjournal.com.br/diversitas_journal/article/view/2420/1954Copyright (c) 2022 Jaqueline Figueredo de Oliveira Costa, Walisson Ferreira da Silva, JOCKELINY MAYARA CÂMARA DOS SANTOS, Jackeline Laurentino, Kevison Romulo da Silva França, Gaus Silvetre de Andrade Lima, Iraildes Pereira Assunçãohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFerreira da Silva, Walisson Câmara dos Santos, Jockeliny MayaraLaurentino, Jackeline da Silva França, Kevison RomuloFigueredo de Oliveira Costa, JaquelineSilvetre de Andrade Lima, Gaus Pereira Assunção, Iraildes2023-02-14T20:21:21Zoai:ojs.diversitasjournal.com.br:article/2420Revistahttps://diversitasjournal.com.br/diversitas_journal/indexPUBhttps://www.e-publicacoes.uerj.br/index.php/muralinternacional/oairevistadiversitasjournal@gmail.com2525-52152525-5215opendoar:2023-02-14T20:21:21Diversitas Journal - Universidade Estadual de Alagoas (UNEAL)false |
dc.title.none.fl_str_mv |
Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas. |
title |
Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas. |
spellingShingle |
Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas. Ferreira da Silva, Walisson antracnose, anonáceas, controle químico anthracnose, annonaceae, chemical control |
title_short |
Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas. |
title_full |
Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas. |
title_fullStr |
Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas. |
title_full_unstemmed |
Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas. |
title_sort |
Avaliação da sensibilidade micelial a fungicidas de Colletotrichum spp. associadas à atemoia no estado de Alagoas. |
author |
Ferreira da Silva, Walisson |
author_facet |
Ferreira da Silva, Walisson Câmara dos Santos, Jockeliny Mayara Laurentino, Jackeline da Silva França, Kevison Romulo Figueredo de Oliveira Costa, Jaqueline Silvetre de Andrade Lima, Gaus Pereira Assunção, Iraildes |
author_role |
author |
author2 |
Câmara dos Santos, Jockeliny Mayara Laurentino, Jackeline da Silva França, Kevison Romulo Figueredo de Oliveira Costa, Jaqueline Silvetre de Andrade Lima, Gaus Pereira Assunção, Iraildes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira da Silva, Walisson Câmara dos Santos, Jockeliny Mayara Laurentino, Jackeline da Silva França, Kevison Romulo Figueredo de Oliveira Costa, Jaqueline Silvetre de Andrade Lima, Gaus Pereira Assunção, Iraildes |
dc.subject.por.fl_str_mv |
antracnose, anonáceas, controle químico anthracnose, annonaceae, chemical control |
topic |
antracnose, anonáceas, controle químico anthracnose, annonaceae, chemical control |
description |
Anthracnose, caused by Colletotrichum spp., is an important disease of atemoya causing damage both pre and post-harvest. Symptoms of this disease range from foliar anthracnose, flower abortion, branch blight and necrotic fruit lesions. Knowing the action of certain fungicides in the fight against the etiological agent is essential to develop control techniques. The objective of this work was to evaluate the mycelial sensitivity of Colletotrichum spp., from atemoya, to the fungicides thiophanate methyl, azoxystrobin and tebuconazole. To determine the sensitivity of Colletotrichum species to fungicides, mycelial growth in synthetic potato-dextrose-agar (PDA) supplemented with fungicide was evaluated. The fungicides were dissolved in dimethylsulfoxide (DMSO) and added to the fluxing culture medium (45°C), to reach concentrations of 0.0, 0.1, 0.5, 1.0, 5.0 and 10 μg/ml-1 of a.i. The mycelial growth of each colony was measured daily to obtain the mycelial growth index (ICM). EC50 was also calculated. After calculating the EC50, the Colletotrichum species were classified into three sensitivity categories, where: EC50: <10 μg/ml-1: high sensitivity (SA); EC50: 10-100 μg/ml-1: moderate sensitivity (MS); EC50: 100-500 μg/ml-1: insensitivity (I). The species C. theobromicola, C. fruticola, C. siamense and C. karstii were highly sensitive to the fungicides tebuconazole and thiophanate methyl. The fungicide azoxystrobin proved to be efficient in the in vitro control of the species C. theobromicola, however, it proved to be inefficient for the control of the species C. siamense. The species C. fruticola and C. karstii were moderately sensitive to the fungicide azoxystrobin. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://diversitasjournal.com.br/diversitas_journal/article/view/2420 10.48017/dj.v8i1.2420 |
url |
https://diversitasjournal.com.br/diversitas_journal/article/view/2420 |
identifier_str_mv |
10.48017/dj.v8i1.2420 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://diversitasjournal.com.br/diversitas_journal/article/view/2420/1953 https://diversitasjournal.com.br/diversitas_journal/article/view/2420/1954 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Alagoas - Eduneal |
publisher.none.fl_str_mv |
Universidade Estadual de Alagoas - Eduneal |
dc.source.none.fl_str_mv |
Diversitas Journal; Vol. 8 No. 1 (2023): Sociedade e ciência: um diálogo necessário Diversitas Journal; Vol. 8 Núm. 1 (2023): Sociedade e ciência: um diálogo necessário Diversitas Journal; v. 8 n. 1 (2023): Sociedade e ciência: um diálogo necessário 2525-5215 10.48017/dj.v8i1 reponame:Diversitas Journal instname:Universidade Estadual de Alagoas (UNEAL) instacron:UNEAL |
instname_str |
Universidade Estadual de Alagoas (UNEAL) |
instacron_str |
UNEAL |
institution |
UNEAL |
reponame_str |
Diversitas Journal |
collection |
Diversitas Journal |
repository.name.fl_str_mv |
Diversitas Journal - Universidade Estadual de Alagoas (UNEAL) |
repository.mail.fl_str_mv |
revistadiversitasjournal@gmail.com |
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1797051272727101440 |