In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Diversitas Journal |
Texto Completo: | https://diversitasjournal.com.br/diversitas_journal/article/view/1592 |
Resumo: | ABSTRACT: The objective of the present study was to evaluate the effects of different culture media on the in vitro germination and initial development of Cattleya nobilior, and to acclimatize this species using soybean straw as a substrate component, aiming the production of plants at large scale to reintroduce it in natural environments. Anatomical characteristics associated with development during acclimatization are also depicted. The influence of Murashige and Skoog, Knudson, and Vacin and Went culture media on the in vitro germination and protocorm development were assessed. Acclimatization was accomplished using different proportions of soybean straw (SS) and Bioplant (BP) as substrates. Anatomical studies were conducted on the leaves of plants during acclimatization. KC was the most suitable culture medium for both germination and initial development of C. nobilior. Regarding acclimatization, it is recommended that a substrate composed of 60% SR and 40% BP be initially used. Upon transfer to shade-house conditions, plants should be grown in a mix of 40% SR: and 60% BP. The leaf anatomical characteristics observed are typical of epiphytic orchids adapted to water-poor environments, which indicates that SR did not negatively affect the development of the species. The in vitro propagation of C. nobilior as described herein is efficient for its multiplication for commercial and conservation purposes and SS can be used as an alternative component of the substrate for its acclimatization. KEYWORDS: Foliar anatomy, culture media, soybean straw. |
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In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado speciesABSTRACT: The objective of the present study was to evaluate the effects of different culture media on the in vitro germination and initial development of Cattleya nobilior, and to acclimatize this species using soybean straw as a substrate component, aiming the production of plants at large scale to reintroduce it in natural environments. Anatomical characteristics associated with development during acclimatization are also depicted. The influence of Murashige and Skoog, Knudson, and Vacin and Went culture media on the in vitro germination and protocorm development were assessed. Acclimatization was accomplished using different proportions of soybean straw (SS) and Bioplant (BP) as substrates. Anatomical studies were conducted on the leaves of plants during acclimatization. KC was the most suitable culture medium for both germination and initial development of C. nobilior. Regarding acclimatization, it is recommended that a substrate composed of 60% SR and 40% BP be initially used. Upon transfer to shade-house conditions, plants should be grown in a mix of 40% SR: and 60% BP. The leaf anatomical characteristics observed are typical of epiphytic orchids adapted to water-poor environments, which indicates that SR did not negatively affect the development of the species. The in vitro propagation of C. nobilior as described herein is efficient for its multiplication for commercial and conservation purposes and SS can be used as an alternative component of the substrate for its acclimatization. KEYWORDS: Foliar anatomy, culture media, soybean straw.RESUMO: O objetivo deste estudo foi avaliar a influência de meios de cultura na germinação in vitro e no desenvolvimento inicial de Cattleya nobilior e aclimatizar essa espécie utilizando palhada de soja (Glycine max) como componente do substrato, com a finalidade de produzir plantas em larga escala para reintroduzi-la em ambientes naturais. Características anatômicas associadas ao desenvolvimento durante a aclimatização também são descritas. A influência dos meios de Murashige e Skoog, Knudson, e Vacin e Went na germinação e desenvolvimento dos protocormos in vitro foi investigada. A aclimatização foi realizada utilizando-se diferentes proporções de palhada de soja (PS) e Bioplant (BP) como substratos. Os estudos anatômicos foram conduzidos com folhas das plantas durante a aclimatização. KC foi o meio de cultura mais adequado tanto para a germinação quanto para o desenvolvimento inicial. Para a aclimatização dessa espécie recomenda-se inicialmente a utilização de um substrato composto por 60% PS e 40% BP. Ao transferir as plantas para viveiro, deve-se utilizar uma mistura de 40% PS e 60% BP. As características anatômicas foliares observadas são típicas de orquídeas epifíticas adaptadas a ambientes pobres em recursos hídricos, o que indica que a OS não afeou negativamente o desenvolvimento da espécie. A propagação in vitro de C nobilior aqui descrita é eficiente para sua multiplicação com fins comerciais e de conservação, e a PS pode ser utilizada como componente alternativo do substrato para sua aclimatização. PALAVRAS-CHAVE: Anatomia foliar, Meio de cultura, Palhada de Soja.Universidade Estadual de Alagoas - Eduneal2021-05-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://diversitasjournal.com.br/diversitas_journal/article/view/159210.17648/diversitas-journal-v6i2-1592Diversitas Journal; v. 6 n. 2 (2021): Educação para o desenvolvimento socioeconômico ; 2167-21912525-521510.17648/diversitas-journal-v6i2reponame:Diversitas Journalinstname:Universidade Estadual de Alagoas (UNEAL)instacron:UNEALporhttps://diversitasjournal.com.br/diversitas_journal/article/view/1592/1378Copyright (c) 2021 Silene Lívia Aires de Oliveira, Kellen Lagares Ferreira Silva, Rafael José de Oliveira, Maíra Jéssica de Souza, Jaderson Roney Gomes de Oliveira, Wagner de Melo Ferreirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira, Silene Lívia Aires deSilva, Kellen Lagares FerreiraOliveira, Rafael José deSouza, Maíra Jéssica deOliveira, Jaderson Roney Gomes deFerreira, Wagner de Melo2021-08-12T20:37:44Zoai:ojs.emnuvens.com.br:article/1592Revistahttps://diversitasjournal.com.br/diversitas_journal/indexPUBhttps://www.e-publicacoes.uerj.br/index.php/muralinternacional/oairevistadiversitasjournal@gmail.com2525-52152525-5215opendoar:2023-01-13T09:47:17.765138Diversitas Journal - Universidade Estadual de Alagoas (UNEAL)false |
dc.title.none.fl_str_mv |
In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species |
title |
In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species |
spellingShingle |
In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species Oliveira, Silene Lívia Aires de |
title_short |
In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species |
title_full |
In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species |
title_fullStr |
In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species |
title_full_unstemmed |
In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species |
title_sort |
In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species |
author |
Oliveira, Silene Lívia Aires de |
author_facet |
Oliveira, Silene Lívia Aires de Silva, Kellen Lagares Ferreira Oliveira, Rafael José de Souza, Maíra Jéssica de Oliveira, Jaderson Roney Gomes de Ferreira, Wagner de Melo |
author_role |
author |
author2 |
Silva, Kellen Lagares Ferreira Oliveira, Rafael José de Souza, Maíra Jéssica de Oliveira, Jaderson Roney Gomes de Ferreira, Wagner de Melo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Silene Lívia Aires de Silva, Kellen Lagares Ferreira Oliveira, Rafael José de Souza, Maíra Jéssica de Oliveira, Jaderson Roney Gomes de Ferreira, Wagner de Melo |
description |
ABSTRACT: The objective of the present study was to evaluate the effects of different culture media on the in vitro germination and initial development of Cattleya nobilior, and to acclimatize this species using soybean straw as a substrate component, aiming the production of plants at large scale to reintroduce it in natural environments. Anatomical characteristics associated with development during acclimatization are also depicted. The influence of Murashige and Skoog, Knudson, and Vacin and Went culture media on the in vitro germination and protocorm development were assessed. Acclimatization was accomplished using different proportions of soybean straw (SS) and Bioplant (BP) as substrates. Anatomical studies were conducted on the leaves of plants during acclimatization. KC was the most suitable culture medium for both germination and initial development of C. nobilior. Regarding acclimatization, it is recommended that a substrate composed of 60% SR and 40% BP be initially used. Upon transfer to shade-house conditions, plants should be grown in a mix of 40% SR: and 60% BP. The leaf anatomical characteristics observed are typical of epiphytic orchids adapted to water-poor environments, which indicates that SR did not negatively affect the development of the species. The in vitro propagation of C. nobilior as described herein is efficient for its multiplication for commercial and conservation purposes and SS can be used as an alternative component of the substrate for its acclimatization. KEYWORDS: Foliar anatomy, culture media, soybean straw. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://diversitasjournal.com.br/diversitas_journal/article/view/1592 10.17648/diversitas-journal-v6i2-1592 |
url |
https://diversitasjournal.com.br/diversitas_journal/article/view/1592 |
identifier_str_mv |
10.17648/diversitas-journal-v6i2-1592 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://diversitasjournal.com.br/diversitas_journal/article/view/1592/1378 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Alagoas - Eduneal |
publisher.none.fl_str_mv |
Universidade Estadual de Alagoas - Eduneal |
dc.source.none.fl_str_mv |
Diversitas Journal; v. 6 n. 2 (2021): Educação para o desenvolvimento socioeconômico ; 2167-2191 2525-5215 10.17648/diversitas-journal-v6i2 reponame:Diversitas Journal instname:Universidade Estadual de Alagoas (UNEAL) instacron:UNEAL |
instname_str |
Universidade Estadual de Alagoas (UNEAL) |
instacron_str |
UNEAL |
institution |
UNEAL |
reponame_str |
Diversitas Journal |
collection |
Diversitas Journal |
repository.name.fl_str_mv |
Diversitas Journal - Universidade Estadual de Alagoas (UNEAL) |
repository.mail.fl_str_mv |
revistadiversitasjournal@gmail.com |
_version_ |
1797051278134607872 |