Bioactivities of extracts from the marine sponge Halichondria panicea

Detalhes bibliográficos
Autor(a) principal: Purushottama,GB
Data de Publicação: 2009
Outros Autores: Venkateshvaran,K, Pani Prasad,K, Nalini,P
Tipo de documento: Artigo
Idioma: eng
Título da fonte: The Journal of venomous animals and toxins including tropical diseases (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300007
Resumo: In the present study, we screened the biological activity of extracts from the marine sponge Halichondria panicea collected in the Arabian Sea. Crude toxin was obtained by methanol, chloroform-methanol (2:1) and aqueous extraction. Subsequently, the protein concentration of each crude extract was determined. The impact of both sponge methanolic and aqueous extracts was found to increase activities of Na+-K+ ATP-ase and Mg++ ATP-ase. In the case of chloroform-methanol extract, higher concentrations increased acetylcholine esterase (AchE) activity. The methanolic and chloroform-methanol extracts exhibited hemolytic activity on chicken and human erythrocytes, whereas the aqueous extract failed to do so. Methanol and aqueous extracts produced an immunostimulating effect and all extracts revealed angiogenic activity. The aqueous extract yielded nine bands by SDS-PAGE on 12% gel.
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spelling Bioactivities of extracts from the marine sponge Halichondria paniceaHalichondria paniceaphagocytosisneuromodulationhemolysisangiogenesisIn the present study, we screened the biological activity of extracts from the marine sponge Halichondria panicea collected in the Arabian Sea. Crude toxin was obtained by methanol, chloroform-methanol (2:1) and aqueous extraction. Subsequently, the protein concentration of each crude extract was determined. The impact of both sponge methanolic and aqueous extracts was found to increase activities of Na+-K+ ATP-ase and Mg++ ATP-ase. In the case of chloroform-methanol extract, higher concentrations increased acetylcholine esterase (AchE) activity. The methanolic and chloroform-methanol extracts exhibited hemolytic activity on chicken and human erythrocytes, whereas the aqueous extract failed to do so. Methanol and aqueous extracts produced an immunostimulating effect and all extracts revealed angiogenic activity. The aqueous extract yielded nine bands by SDS-PAGE on 12% gel.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300007Journal of Venomous Animals and Toxins including Tropical Diseases v.15 n.3 2009reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992009000300007info:eu-repo/semantics/openAccessPurushottama,GBVenkateshvaran,KPani Prasad,KNalini,Peng2009-09-14T00:00:00Zoai:scielo:S1678-91992009000300007Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2009-09-14T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bioactivities of extracts from the marine sponge Halichondria panicea
title Bioactivities of extracts from the marine sponge Halichondria panicea
spellingShingle Bioactivities of extracts from the marine sponge Halichondria panicea
Purushottama,GB
Halichondria panicea
phagocytosis
neuromodulation
hemolysis
angiogenesis
title_short Bioactivities of extracts from the marine sponge Halichondria panicea
title_full Bioactivities of extracts from the marine sponge Halichondria panicea
title_fullStr Bioactivities of extracts from the marine sponge Halichondria panicea
title_full_unstemmed Bioactivities of extracts from the marine sponge Halichondria panicea
title_sort Bioactivities of extracts from the marine sponge Halichondria panicea
author Purushottama,GB
author_facet Purushottama,GB
Venkateshvaran,K
Pani Prasad,K
Nalini,P
author_role author
author2 Venkateshvaran,K
Pani Prasad,K
Nalini,P
author2_role author
author
author
dc.contributor.author.fl_str_mv Purushottama,GB
Venkateshvaran,K
Pani Prasad,K
Nalini,P
dc.subject.por.fl_str_mv Halichondria panicea
phagocytosis
neuromodulation
hemolysis
angiogenesis
topic Halichondria panicea
phagocytosis
neuromodulation
hemolysis
angiogenesis
description In the present study, we screened the biological activity of extracts from the marine sponge Halichondria panicea collected in the Arabian Sea. Crude toxin was obtained by methanol, chloroform-methanol (2:1) and aqueous extraction. Subsequently, the protein concentration of each crude extract was determined. The impact of both sponge methanolic and aqueous extracts was found to increase activities of Na+-K+ ATP-ase and Mg++ ATP-ase. In the case of chloroform-methanol extract, higher concentrations increased acetylcholine esterase (AchE) activity. The methanolic and chloroform-methanol extracts exhibited hemolytic activity on chicken and human erythrocytes, whereas the aqueous extract failed to do so. Methanol and aqueous extracts produced an immunostimulating effect and all extracts revealed angiogenic activity. The aqueous extract yielded nine bands by SDS-PAGE on 12% gel.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-91992009000300007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
dc.source.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases v.15 n.3 2009
reponame:The Journal of venomous animals and toxins including tropical diseases (Online)
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str The Journal of venomous animals and toxins including tropical diseases (Online)
collection The Journal of venomous animals and toxins including tropical diseases (Online)
repository.name.fl_str_mv The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv ||editorial@jvat.org.br
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