On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)

Detalhes bibliográficos
Autor(a) principal: Kanagarajan,U
Data de Publicação: 2008
Outros Autores: Bragadeeswaran,S, Venkateshvaran,K
Tipo de documento: Artigo
Idioma: eng
Título da fonte: The Journal of venomous animals and toxins including tropical diseases (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300005
Resumo: Whole-body extracts in methanol were obtained from the starfish Stellaster equestris. The crude toxin was fractionated stepwise using diethylaminoethyl (DEAE) cellulose column chromatography. The crude toxin was lethal to male albino mice at a dose of 1.00 mL (containing 531.0 µg/mL protein) when injected intraperitoneally (IP) but the toxicity was abolished in all cases except one upon fractionation. The crude toxin and all the adsorbed fractions exhibited potent hemolytic activity on chicken, goat and human blood. However, group B human erythrocytes were resistant to lysis by all fractions and group O by most of the fractions. Paw edema in mice was caused by the crude toxin and all fractions. Pheniramine maleate and piroxicam blocked the toxicity when administered earlier than, or along with, the crude or fractionated toxins but not when administered after the envenomation. Pretreatment with either of these drugs also blocked edema formation.
id UNESP-11_84efe933d06fd2f8c676f2b9afc0d959
oai_identifier_str oai:scielo:S1678-91992008000300005
network_acronym_str UNESP-11
network_name_str The Journal of venomous animals and toxins including tropical diseases (Online)
repository_id_str
spelling On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)starfishtoxicityhemolysishuman blood groupspaw edemaWhole-body extracts in methanol were obtained from the starfish Stellaster equestris. The crude toxin was fractionated stepwise using diethylaminoethyl (DEAE) cellulose column chromatography. The crude toxin was lethal to male albino mice at a dose of 1.00 mL (containing 531.0 µg/mL protein) when injected intraperitoneally (IP) but the toxicity was abolished in all cases except one upon fractionation. The crude toxin and all the adsorbed fractions exhibited potent hemolytic activity on chicken, goat and human blood. However, group B human erythrocytes were resistant to lysis by all fractions and group O by most of the fractions. Paw edema in mice was caused by the crude toxin and all fractions. Pheniramine maleate and piroxicam blocked the toxicity when administered earlier than, or along with, the crude or fractionated toxins but not when administered after the envenomation. Pretreatment with either of these drugs also blocked edema formation.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300005Journal of Venomous Animals and Toxins including Tropical Diseases v.14 n.3 2008reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992008000300005info:eu-repo/semantics/openAccessKanagarajan,UBragadeeswaran,SVenkateshvaran,Keng2008-09-09T00:00:00Zoai:scielo:S1678-91992008000300005Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2008-09-09T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)
title On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)
spellingShingle On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)
Kanagarajan,U
starfish
toxicity
hemolysis
human blood groups
paw edema
title_short On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)
title_full On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)
title_fullStr On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)
title_full_unstemmed On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)
title_sort On some toxinological aspects of the starfish Stellaster equestris (Retzius, 1805)
author Kanagarajan,U
author_facet Kanagarajan,U
Bragadeeswaran,S
Venkateshvaran,K
author_role author
author2 Bragadeeswaran,S
Venkateshvaran,K
author2_role author
author
dc.contributor.author.fl_str_mv Kanagarajan,U
Bragadeeswaran,S
Venkateshvaran,K
dc.subject.por.fl_str_mv starfish
toxicity
hemolysis
human blood groups
paw edema
topic starfish
toxicity
hemolysis
human blood groups
paw edema
description Whole-body extracts in methanol were obtained from the starfish Stellaster equestris. The crude toxin was fractionated stepwise using diethylaminoethyl (DEAE) cellulose column chromatography. The crude toxin was lethal to male albino mice at a dose of 1.00 mL (containing 531.0 µg/mL protein) when injected intraperitoneally (IP) but the toxicity was abolished in all cases except one upon fractionation. The crude toxin and all the adsorbed fractions exhibited potent hemolytic activity on chicken, goat and human blood. However, group B human erythrocytes were resistant to lysis by all fractions and group O by most of the fractions. Paw edema in mice was caused by the crude toxin and all fractions. Pheniramine maleate and piroxicam blocked the toxicity when administered earlier than, or along with, the crude or fractionated toxins but not when administered after the envenomation. Pretreatment with either of these drugs also blocked edema formation.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-91992008000300005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
dc.source.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases v.14 n.3 2008
reponame:The Journal of venomous animals and toxins including tropical diseases (Online)
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str The Journal of venomous animals and toxins including tropical diseases (Online)
collection The Journal of venomous animals and toxins including tropical diseases (Online)
repository.name.fl_str_mv The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv ||editorial@jvat.org.br
_version_ 1748958538323984384

Registros relacionados