Fibrin biopolymer as scaffold candidate to treat bone defects in rats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100320 |
Resumo: | ABSTRACT Background: Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery properties. The regenerative potential of an association of FBP, biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSCs) was evaluated in defects of rat femurs. Methods: Adult male Wistar rats were submitted to a 5-mm defect in the femur. This was filled with the following materials and/or associations: BPC; FBP and BCP; FBP and MSCs; and BCP, FBP and MSCs. Bone defect without filling was defined as the control group. Thirty and sixty days after the procedure, animals were euthanatized and subjected to computed tomography, scanning electron microscopy and qualitative and quantitative histological analysis. Results: It was shown that FBP is a suitable scaffold for bone defects due to the formation of a stable clot that facilitates the handling and optimizes the surgical procedures, allowing also cell adhesion and proliferation. The association between the materials was biocompatible. Progressive deposition of bone matrix was higher in the group treated with FBP and MSCs. Differentiation of mesenchymal stem cells into osteogenic lineage was not necessary to stimulate bone formation. Conclusions: FBP proved to be an excellent scaffold candidate for bone repair therapies due to application ease and biocompatibility with synthetic calcium-based materials. The satisfactory results obtained by the association of FBP with MSCs may provide a more effective and less costly new approach for bone tissue engineering. |
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The Journal of venomous animals and toxins including tropical diseases (Online) |
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|
spelling |
Fibrin biopolymer as scaffold candidate to treat bone defects in ratsBone regenerationBiomaterialsFibrin sealantFibrin biopolymerBiphasic calcium phosphateMesenchymal stem cellsABSTRACT Background: Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery properties. The regenerative potential of an association of FBP, biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSCs) was evaluated in defects of rat femurs. Methods: Adult male Wistar rats were submitted to a 5-mm defect in the femur. This was filled with the following materials and/or associations: BPC; FBP and BCP; FBP and MSCs; and BCP, FBP and MSCs. Bone defect without filling was defined as the control group. Thirty and sixty days after the procedure, animals were euthanatized and subjected to computed tomography, scanning electron microscopy and qualitative and quantitative histological analysis. Results: It was shown that FBP is a suitable scaffold for bone defects due to the formation of a stable clot that facilitates the handling and optimizes the surgical procedures, allowing also cell adhesion and proliferation. The association between the materials was biocompatible. Progressive deposition of bone matrix was higher in the group treated with FBP and MSCs. Differentiation of mesenchymal stem cells into osteogenic lineage was not necessary to stimulate bone formation. Conclusions: FBP proved to be an excellent scaffold candidate for bone repair therapies due to application ease and biocompatibility with synthetic calcium-based materials. The satisfactory results obtained by the association of FBP with MSCs may provide a more effective and less costly new approach for bone tissue engineering.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100320Journal of Venomous Animals and Toxins including Tropical Diseases v.25 2019reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/1678-9199-jvatitd-2019-0027info:eu-repo/semantics/openAccessCassaro,Claudia VilalvaJustulin Jr.,Luis AntonioLima,Patrícia Rodrigues deGolim,Marjorie de AssisBiscola,Natália PerussiCastro,Mateus Vidigal deOliveira,Alexandre Leite Rodrigues deDoiche,Danuta PulzPereira,Elenize JamasFerreira Jr.,Rui SeabraBarraviera,Beneditoeng2019-11-01T00:00:00Zoai:scielo:S1678-91992019000100320Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2019-11-01T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fibrin biopolymer as scaffold candidate to treat bone defects in rats |
title |
Fibrin biopolymer as scaffold candidate to treat bone defects in rats |
spellingShingle |
Fibrin biopolymer as scaffold candidate to treat bone defects in rats Cassaro,Claudia Vilalva Bone regeneration Biomaterials Fibrin sealant Fibrin biopolymer Biphasic calcium phosphate Mesenchymal stem cells |
title_short |
Fibrin biopolymer as scaffold candidate to treat bone defects in rats |
title_full |
Fibrin biopolymer as scaffold candidate to treat bone defects in rats |
title_fullStr |
Fibrin biopolymer as scaffold candidate to treat bone defects in rats |
title_full_unstemmed |
Fibrin biopolymer as scaffold candidate to treat bone defects in rats |
title_sort |
Fibrin biopolymer as scaffold candidate to treat bone defects in rats |
author |
Cassaro,Claudia Vilalva |
author_facet |
Cassaro,Claudia Vilalva Justulin Jr.,Luis Antonio Lima,Patrícia Rodrigues de Golim,Marjorie de Assis Biscola,Natália Perussi Castro,Mateus Vidigal de Oliveira,Alexandre Leite Rodrigues de Doiche,Danuta Pulz Pereira,Elenize Jamas Ferreira Jr.,Rui Seabra Barraviera,Benedito |
author_role |
author |
author2 |
Justulin Jr.,Luis Antonio Lima,Patrícia Rodrigues de Golim,Marjorie de Assis Biscola,Natália Perussi Castro,Mateus Vidigal de Oliveira,Alexandre Leite Rodrigues de Doiche,Danuta Pulz Pereira,Elenize Jamas Ferreira Jr.,Rui Seabra Barraviera,Benedito |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cassaro,Claudia Vilalva Justulin Jr.,Luis Antonio Lima,Patrícia Rodrigues de Golim,Marjorie de Assis Biscola,Natália Perussi Castro,Mateus Vidigal de Oliveira,Alexandre Leite Rodrigues de Doiche,Danuta Pulz Pereira,Elenize Jamas Ferreira Jr.,Rui Seabra Barraviera,Benedito |
dc.subject.por.fl_str_mv |
Bone regeneration Biomaterials Fibrin sealant Fibrin biopolymer Biphasic calcium phosphate Mesenchymal stem cells |
topic |
Bone regeneration Biomaterials Fibrin sealant Fibrin biopolymer Biphasic calcium phosphate Mesenchymal stem cells |
description |
ABSTRACT Background: Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery properties. The regenerative potential of an association of FBP, biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSCs) was evaluated in defects of rat femurs. Methods: Adult male Wistar rats were submitted to a 5-mm defect in the femur. This was filled with the following materials and/or associations: BPC; FBP and BCP; FBP and MSCs; and BCP, FBP and MSCs. Bone defect without filling was defined as the control group. Thirty and sixty days after the procedure, animals were euthanatized and subjected to computed tomography, scanning electron microscopy and qualitative and quantitative histological analysis. Results: It was shown that FBP is a suitable scaffold for bone defects due to the formation of a stable clot that facilitates the handling and optimizes the surgical procedures, allowing also cell adhesion and proliferation. The association between the materials was biocompatible. Progressive deposition of bone matrix was higher in the group treated with FBP and MSCs. Differentiation of mesenchymal stem cells into osteogenic lineage was not necessary to stimulate bone formation. Conclusions: FBP proved to be an excellent scaffold candidate for bone repair therapies due to application ease and biocompatibility with synthetic calcium-based materials. The satisfactory results obtained by the association of FBP with MSCs may provide a more effective and less costly new approach for bone tissue engineering. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100320 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100320 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-9199-jvatitd-2019-0027 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.25 2019 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
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1748958540601491456 |