In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100501 |
Resumo: | Abstract Background Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response of these patients is unclear. Therefore, the aim of the present study was to characterize the cellular immune response in the skin lesions of patients affected by NUCL. Methods Twenty biopsies were processed by immunohistochemistry using primary antibodies to T lymphocytes (CD4, CD8), NK cells, B lymphocytes, macrophages, nitric oxide synthase and interferon-gamma. Results Immunohistochemistry revealed higher expression of all cellular types and molecules (IFN-γ, iNOS) in the dermis of diseased skin compared to the skin of healthy individuals (p < 0.05). Morphometric analysis performed in the skin lesions sections showed the predominance of CD8+ T lymphocytes in the mononuclear infiltrate, followed by macrophages, mostly iNOS+, a response that could be mediated by IFN-γ. Conclusion Our study improves knowledge of the cellular immune response in non-ulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Central America and pointed to the pivotal participation of CD8+ T lymphocytes in the host defense mechanisms against the parasite in patients with NUCL. |
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The Journal of venomous animals and toxins including tropical diseases (Online) |
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In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infectionNon-ulcerated cutaneous leishmaniasisAtypical cutaneous leishmaniasisLeishmania infantumchagasiCellular immune responseImmunohistochemistryHondurasAbstract Background Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response of these patients is unclear. Therefore, the aim of the present study was to characterize the cellular immune response in the skin lesions of patients affected by NUCL. Methods Twenty biopsies were processed by immunohistochemistry using primary antibodies to T lymphocytes (CD4, CD8), NK cells, B lymphocytes, macrophages, nitric oxide synthase and interferon-gamma. Results Immunohistochemistry revealed higher expression of all cellular types and molecules (IFN-γ, iNOS) in the dermis of diseased skin compared to the skin of healthy individuals (p < 0.05). Morphometric analysis performed in the skin lesions sections showed the predominance of CD8+ T lymphocytes in the mononuclear infiltrate, followed by macrophages, mostly iNOS+, a response that could be mediated by IFN-γ. Conclusion Our study improves knowledge of the cellular immune response in non-ulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Central America and pointed to the pivotal participation of CD8+ T lymphocytes in the host defense mechanisms against the parasite in patients with NUCL.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2021-01-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100501Journal of Venomous Animals and Toxins including Tropical Diseases v.27 2021reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/1678-9199-jvatitd-2020-0149info:eu-repo/semantics/openAccessSandoval,CarmenAraujo,GabrielaSosa,WilfredoAvalos,SaraSilveira,FernandoCorbett,CarlosZúniga,ConcepciónLaurenti,Marciaeng2021-02-23T00:00:00Zoai:scielo:S1678-91992021000100501Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2021-02-23T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
title |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
spellingShingle |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection Sandoval,Carmen Non-ulcerated cutaneous leishmaniasis Atypical cutaneous leishmaniasis Leishmania infantumchagasi Cellular immune response Immunohistochemistry Honduras |
title_short |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
title_full |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
title_fullStr |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
title_full_unstemmed |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
title_sort |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
author |
Sandoval,Carmen |
author_facet |
Sandoval,Carmen Araujo,Gabriela Sosa,Wilfredo Avalos,Sara Silveira,Fernando Corbett,Carlos Zúniga,Concepción Laurenti,Marcia |
author_role |
author |
author2 |
Araujo,Gabriela Sosa,Wilfredo Avalos,Sara Silveira,Fernando Corbett,Carlos Zúniga,Concepción Laurenti,Marcia |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Sandoval,Carmen Araujo,Gabriela Sosa,Wilfredo Avalos,Sara Silveira,Fernando Corbett,Carlos Zúniga,Concepción Laurenti,Marcia |
dc.subject.por.fl_str_mv |
Non-ulcerated cutaneous leishmaniasis Atypical cutaneous leishmaniasis Leishmania infantumchagasi Cellular immune response Immunohistochemistry Honduras |
topic |
Non-ulcerated cutaneous leishmaniasis Atypical cutaneous leishmaniasis Leishmania infantumchagasi Cellular immune response Immunohistochemistry Honduras |
description |
Abstract Background Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response of these patients is unclear. Therefore, the aim of the present study was to characterize the cellular immune response in the skin lesions of patients affected by NUCL. Methods Twenty biopsies were processed by immunohistochemistry using primary antibodies to T lymphocytes (CD4, CD8), NK cells, B lymphocytes, macrophages, nitric oxide synthase and interferon-gamma. Results Immunohistochemistry revealed higher expression of all cellular types and molecules (IFN-γ, iNOS) in the dermis of diseased skin compared to the skin of healthy individuals (p < 0.05). Morphometric analysis performed in the skin lesions sections showed the predominance of CD8+ T lymphocytes in the mononuclear infiltrate, followed by macrophages, mostly iNOS+, a response that could be mediated by IFN-γ. Conclusion Our study improves knowledge of the cellular immune response in non-ulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Central America and pointed to the pivotal participation of CD8+ T lymphocytes in the host defense mechanisms against the parasite in patients with NUCL. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100501 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100501 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-9199-jvatitd-2020-0149 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.27 2021 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
_version_ |
1748958541083836416 |