Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100318 |
Resumo: | ABSTRACT Background: Bufonid parotoid macrogland secretion contains several low molecular mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is poorly understood. Herein, we applied a sample preparation methodology that allows the analysis of viscous matrices in order to examine its proteins. Methods: Duttaphrynus melanostictus parotoid macrogland secretion was submitted to ion-exchange batch sample preparation, yielding two fractions: salt-displaced fraction and acid-displaced fraction. Each sample was then fractionated by anionic-exchange chromatography, followed by in-solution proteomic analysis. Results: Forty-two proteins could be identified, such as acyl-CoA-binding protein, alcohol dehydrogenase, calmodulin, galectin and histone. Moreover, de novo analyses yielded 153 peptides, whereas BLAST analyses corroborated some of the proteomic-identified proteins. Furthermore, the de novo peptide analyses indicate the presence of proteins related to apoptosis, cellular structure, catalysis and transport processes. Conclusions: Proper sample preparation allowed the proteomic and de novo identification of different proteins in the D. melanostictus parotoid macrogland secretion. These results may increase the knowledge about the universe of molecules that compose amphibian skin secretion, as well as to understand their biological/physiological role in the granular gland. |
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The Journal of venomous animals and toxins including tropical diseases (Online) |
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Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictusAmphibian skin secretionBufonidaeDuttaphrynus melanostictusProteomicsBatch chromatographyAsian common toadABSTRACT Background: Bufonid parotoid macrogland secretion contains several low molecular mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is poorly understood. Herein, we applied a sample preparation methodology that allows the analysis of viscous matrices in order to examine its proteins. Methods: Duttaphrynus melanostictus parotoid macrogland secretion was submitted to ion-exchange batch sample preparation, yielding two fractions: salt-displaced fraction and acid-displaced fraction. Each sample was then fractionated by anionic-exchange chromatography, followed by in-solution proteomic analysis. Results: Forty-two proteins could be identified, such as acyl-CoA-binding protein, alcohol dehydrogenase, calmodulin, galectin and histone. Moreover, de novo analyses yielded 153 peptides, whereas BLAST analyses corroborated some of the proteomic-identified proteins. Furthermore, the de novo peptide analyses indicate the presence of proteins related to apoptosis, cellular structure, catalysis and transport processes. Conclusions: Proper sample preparation allowed the proteomic and de novo identification of different proteins in the D. melanostictus parotoid macrogland secretion. These results may increase the knowledge about the universe of molecules that compose amphibian skin secretion, as well as to understand their biological/physiological role in the granular gland.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100318Journal of Venomous Animals and Toxins including Tropical Diseases v.25 2019reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/1678-9199-jvatitd-2019-0029info:eu-repo/semantics/openAccessMariano,Douglas Oscar CeolinMessias,Marcela Di GiacomoSpencer,Patrick JackPimenta,Daniel Carvalhoeng2019-08-15T00:00:00Zoai:scielo:S1678-91992019000100318Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2019-08-15T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus |
title |
Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus |
spellingShingle |
Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus Mariano,Douglas Oscar Ceolin Amphibian skin secretion Bufonidae Duttaphrynus melanostictus Proteomics Batch chromatography Asian common toad |
title_short |
Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus |
title_full |
Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus |
title_fullStr |
Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus |
title_full_unstemmed |
Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus |
title_sort |
Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus |
author |
Mariano,Douglas Oscar Ceolin |
author_facet |
Mariano,Douglas Oscar Ceolin Messias,Marcela Di Giacomo Spencer,Patrick Jack Pimenta,Daniel Carvalho |
author_role |
author |
author2 |
Messias,Marcela Di Giacomo Spencer,Patrick Jack Pimenta,Daniel Carvalho |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Mariano,Douglas Oscar Ceolin Messias,Marcela Di Giacomo Spencer,Patrick Jack Pimenta,Daniel Carvalho |
dc.subject.por.fl_str_mv |
Amphibian skin secretion Bufonidae Duttaphrynus melanostictus Proteomics Batch chromatography Asian common toad |
topic |
Amphibian skin secretion Bufonidae Duttaphrynus melanostictus Proteomics Batch chromatography Asian common toad |
description |
ABSTRACT Background: Bufonid parotoid macrogland secretion contains several low molecular mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is poorly understood. Herein, we applied a sample preparation methodology that allows the analysis of viscous matrices in order to examine its proteins. Methods: Duttaphrynus melanostictus parotoid macrogland secretion was submitted to ion-exchange batch sample preparation, yielding two fractions: salt-displaced fraction and acid-displaced fraction. Each sample was then fractionated by anionic-exchange chromatography, followed by in-solution proteomic analysis. Results: Forty-two proteins could be identified, such as acyl-CoA-binding protein, alcohol dehydrogenase, calmodulin, galectin and histone. Moreover, de novo analyses yielded 153 peptides, whereas BLAST analyses corroborated some of the proteomic-identified proteins. Furthermore, the de novo peptide analyses indicate the presence of proteins related to apoptosis, cellular structure, catalysis and transport processes. Conclusions: Proper sample preparation allowed the proteomic and de novo identification of different proteins in the D. melanostictus parotoid macrogland secretion. These results may increase the knowledge about the universe of molecules that compose amphibian skin secretion, as well as to understand their biological/physiological role in the granular gland. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100318 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100318 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-9199-jvatitd-2019-0029 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.25 2019 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
_version_ |
1748958540597297152 |