Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens

Detalhes bibliográficos
Autor(a) principal: Richini-Pereira,VB
Data de Publicação: 2010
Outros Autores: Bosco,SMG, Theodoro,RC, Barrozo,L, Bagagli,E
Tipo de documento: Artigo
Idioma: eng
Título da fonte: The Journal of venomous animals and toxins including tropical diseases (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400011
Resumo: Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several fungi, as well as determining natural reservoirs in wild animals and facilitating the understanding of host-pathogen relationships.
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spelling Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogensroad-killed animalsfungal pathogenseco-epidemiologyPCRRoad-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several fungi, as well as determining natural reservoirs in wild animals and facilitating the understanding of host-pathogen relationships.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400011Journal of Venomous Animals and Toxins including Tropical Diseases v.16 n.4 2010reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992010000400011info:eu-repo/semantics/openAccessRichini-Pereira,VBBosco,SMGTheodoro,RCBarrozo,LBagagli,Eeng2011-09-30T00:00:00Zoai:scielo:S1678-91992010000400011Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2011-09-30T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
title Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
spellingShingle Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
Richini-Pereira,VB
road-killed animals
fungal pathogens
eco-epidemiology
PCR
title_short Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
title_full Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
title_fullStr Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
title_full_unstemmed Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
title_sort Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
author Richini-Pereira,VB
author_facet Richini-Pereira,VB
Bosco,SMG
Theodoro,RC
Barrozo,L
Bagagli,E
author_role author
author2 Bosco,SMG
Theodoro,RC
Barrozo,L
Bagagli,E
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Richini-Pereira,VB
Bosco,SMG
Theodoro,RC
Barrozo,L
Bagagli,E
dc.subject.por.fl_str_mv road-killed animals
fungal pathogens
eco-epidemiology
PCR
topic road-killed animals
fungal pathogens
eco-epidemiology
PCR
description Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several fungi, as well as determining natural reservoirs in wild animals and facilitating the understanding of host-pathogen relationships.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-91992010000400011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
dc.source.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases v.16 n.4 2010
reponame:The Journal of venomous animals and toxins including tropical diseases (Online)
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str The Journal of venomous animals and toxins including tropical diseases (Online)
collection The Journal of venomous animals and toxins including tropical diseases (Online)
repository.name.fl_str_mv The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv ||editorial@jvat.org.br
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