Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Motriz (Online) |
Texto Completo: | https://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/814 |
Resumo: | The termination of exercise training (detraining) results in rapid fat accretion, weight gain and insulin resistance in both humans and rats. There is evident relationship between physical inactivity and insulin resistance. Different mechanisms may be involved in insulin resistance in this animal model. The aim of this study was to investigate the PI3-kinase/Akt and CAP/Cbl signaling pathways both involved with insulin-stimulated glucose uptake in white adipose tissue in detrained and sedentary animals submitted a high-fat diet. Wistar rats were submitted to swimming training during 8 weeks. Next this period the animals stop the training and received a rich-fat diet. The proteins from the insulin signaling pathway were analyzed by immunoprecipitation and immunoblotting. The results demonstrated that detraining result in an increased body mass and rapid body fat accretion. This fact was associated with increases insulin responsiveness in adipose tissue through IRS/PI3-Kinase/Akt pathway in detrained rats fed with a rich-fat diet. In addition, the CAP/Cbl pathway in adipose tissue was more insulin responsives in the detrained animals feeding with a rich-fat diet than S-DHL animals. In conclusion, the cessation of exercise is accompanied by increased body mass and rapid fat accretion and this aspect to have relation at least in part to increased insulin responsiveness in adipose tissue through CAP/Cbl pathway. |
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Motriz (Online) |
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Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat dietMecanismos moleculares de indução de obesidade e resistência à insulina em animais destreinados submetidos a uma dieta rica em lipídes.Destreinamento. Obesidade. Resistência à insulina.The termination of exercise training (detraining) results in rapid fat accretion, weight gain and insulin resistance in both humans and rats. There is evident relationship between physical inactivity and insulin resistance. Different mechanisms may be involved in insulin resistance in this animal model. The aim of this study was to investigate the PI3-kinase/Akt and CAP/Cbl signaling pathways both involved with insulin-stimulated glucose uptake in white adipose tissue in detrained and sedentary animals submitted a high-fat diet. Wistar rats were submitted to swimming training during 8 weeks. Next this period the animals stop the training and received a rich-fat diet. The proteins from the insulin signaling pathway were analyzed by immunoprecipitation and immunoblotting. The results demonstrated that detraining result in an increased body mass and rapid body fat accretion. This fact was associated with increases insulin responsiveness in adipose tissue through IRS/PI3-Kinase/Akt pathway in detrained rats fed with a rich-fat diet. In addition, the CAP/Cbl pathway in adipose tissue was more insulin responsives in the detrained animals feeding with a rich-fat diet than S-DHL animals. In conclusion, the cessation of exercise is accompanied by increased body mass and rapid fat accretion and this aspect to have relation at least in part to increased insulin responsiveness in adipose tissue through CAP/Cbl pathway.A cessação do treinamento físico (destreinamento) resulta em rápido acréscimo da massa adiposa, ganho de peso e resistência à insulina tanto em humanos quanto em animais. No entanto, os mecanismos moleculares envolvidos nesse processo permanecem desconhecidos. Diferentes proteínas intracelulares podem estar envolvidas no processo de aquisição de ganho de peso e diminuição na ação da insulina nesse modelo animal. Este estudo teve como objetivos investigar as vias PI 3-quinase/Akt e CAP/Cbl, ambas importantes na captação ou utilização de glicose estimulada por insulina no tecido muscular adiposo branco. Utilizou-se, ratos Wistar que foram submetidos a um protocolo de exercício de natação por 8 semanas. Posteriormente os animais foram destreinados e nesse mesmo período de cessamento do programa de exercício foi oferecida aos ratos uma dieta rica em lipídes. Para análise das proteínas de interesse, foi realizado o método de imunoblot e imunoprecipitação. Verifica-se, através dos resultados obtidos, que animais destreinados tem um ganho de peso e de gordura epididimal mais acentuado comparado a animais sedentários. Esse fato foi associado a uma maior responsividade à insulina no tecido adiposo através da via IRS/PI 3-quinase/Akt dos animais destreinados submetidos à dieta rica em lipídes. Além disso, verifica-se, que a via CAP/Cbl encontra-se mais responsiva à insulina no tecido adiposo de animais destreinados em relação aos outros grupos experimentais. Conclui-se, que o destreinamento físico é acompanhado por um rápido período de ganho de peso e de massa adiposa e essas adaptações deve-se no mínimo em parte pela maior responsividade à insulina da via CAP/Cbl em tecido adiposo branco.Universidade Estadual Paulista - Câmpus de Rio Claro2007-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionDocumento Avaliado por Paresapplication/pdfhttps://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/81410.5016/814Motriz. Journal of Physical Education. UNESP; V. 13, N. 2 Suplemento (2007); S019-S028Motriz Revista de Educação Física; V. 13, N. 2 Suplemento (2007); S019-S0281980-6574reponame:Motriz (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESPporhttps://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/814/751Pauli, José RodrigoEsper Cintra, DennysRochete Ropelle, EduardoTeodoro de Souza, CláudioContin Moraes, JulianaAbdalla Saad, Mário Joséinfo:eu-repo/semantics/openAccess2013-04-19T15:14:35Zoai:periodicos.rc.biblioteca.unesp.br:article/814Revistahttp://www.periodicos.rc.biblioteca.unesp.br/index.php/motrizPUBhttps://old.scielo.br/oai/scielo-oai.phpmotriz@rc.unesp.br||mauerber@rc.unesp.br||azanesco@rc.unesp.br1980-65741415-9805opendoar:2013-04-19T15:14:35Motriz (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet Mecanismos moleculares de indução de obesidade e resistência à insulina em animais destreinados submetidos a uma dieta rica em lipídes. |
title |
Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet |
spellingShingle |
Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet Pauli, José Rodrigo Destreinamento. Obesidade. Resistência à insulina. |
title_short |
Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet |
title_full |
Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet |
title_fullStr |
Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet |
title_full_unstemmed |
Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet |
title_sort |
Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet |
author |
Pauli, José Rodrigo |
author_facet |
Pauli, José Rodrigo Esper Cintra, Dennys Rochete Ropelle, Eduardo Teodoro de Souza, Cláudio Contin Moraes, Juliana Abdalla Saad, Mário José |
author_role |
author |
author2 |
Esper Cintra, Dennys Rochete Ropelle, Eduardo Teodoro de Souza, Cláudio Contin Moraes, Juliana Abdalla Saad, Mário José |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pauli, José Rodrigo Esper Cintra, Dennys Rochete Ropelle, Eduardo Teodoro de Souza, Cláudio Contin Moraes, Juliana Abdalla Saad, Mário José |
dc.subject.por.fl_str_mv |
Destreinamento. Obesidade. Resistência à insulina. |
topic |
Destreinamento. Obesidade. Resistência à insulina. |
description |
The termination of exercise training (detraining) results in rapid fat accretion, weight gain and insulin resistance in both humans and rats. There is evident relationship between physical inactivity and insulin resistance. Different mechanisms may be involved in insulin resistance in this animal model. The aim of this study was to investigate the PI3-kinase/Akt and CAP/Cbl signaling pathways both involved with insulin-stimulated glucose uptake in white adipose tissue in detrained and sedentary animals submitted a high-fat diet. Wistar rats were submitted to swimming training during 8 weeks. Next this period the animals stop the training and received a rich-fat diet. The proteins from the insulin signaling pathway were analyzed by immunoprecipitation and immunoblotting. The results demonstrated that detraining result in an increased body mass and rapid body fat accretion. This fact was associated with increases insulin responsiveness in adipose tissue through IRS/PI3-Kinase/Akt pathway in detrained rats fed with a rich-fat diet. In addition, the CAP/Cbl pathway in adipose tissue was more insulin responsives in the detrained animals feeding with a rich-fat diet than S-DHL animals. In conclusion, the cessation of exercise is accompanied by increased body mass and rapid fat accretion and this aspect to have relation at least in part to increased insulin responsiveness in adipose tissue through CAP/Cbl pathway. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-12-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Documento Avaliado por Pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/814 10.5016/814 |
url |
https://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/814 |
identifier_str_mv |
10.5016/814 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/814/751 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista - Câmpus de Rio Claro |
publisher.none.fl_str_mv |
Universidade Estadual Paulista - Câmpus de Rio Claro |
dc.source.none.fl_str_mv |
Motriz. Journal of Physical Education. UNESP; V. 13, N. 2 Suplemento (2007); S019-S028 Motriz Revista de Educação Física; V. 13, N. 2 Suplemento (2007); S019-S028 1980-6574 reponame:Motriz (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Motriz (Online) |
collection |
Motriz (Online) |
repository.name.fl_str_mv |
Motriz (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
motriz@rc.unesp.br||mauerber@rc.unesp.br||azanesco@rc.unesp.br |
_version_ |
1800215768855478272 |