Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet

Detalhes bibliográficos
Autor(a) principal: Pauli, José Rodrigo
Data de Publicação: 2007
Outros Autores: Esper Cintra, Dennys, Rochete Ropelle, Eduardo, Teodoro de Souza, Cláudio, Contin Moraes, Juliana, Abdalla Saad, Mário José
Tipo de documento: Artigo
Idioma: por
Título da fonte: Motriz (Online)
Texto Completo: https://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/814
Resumo: The termination of exercise training (detraining) results in rapid fat accretion, weight gain and insulin resistance in both humans and rats. There is evident relationship between physical inactivity and insulin resistance. Different mechanisms may be involved in insulin resistance in this animal model. The aim of this study was to investigate the PI3-kinase/Akt and CAP/Cbl signaling pathways both involved with insulin-stimulated glucose uptake in white adipose tissue in detrained and sedentary animals submitted a high-fat diet. Wistar rats were submitted to swimming training during 8 weeks. Next this period the animals stop the training and received a rich-fat diet. The proteins from the insulin signaling pathway were analyzed by immunoprecipitation and immunoblotting. The results demonstrated that detraining result in an increased body mass and rapid body fat accretion. This fact was associated with increases insulin responsiveness in adipose tissue through IRS/PI3-Kinase/Akt pathway in detrained rats fed with a rich-fat diet. In addition, the CAP/Cbl pathway in adipose tissue was more insulin responsives in the detrained animals feeding with a rich-fat diet than S-DHL animals. In conclusion, the cessation of exercise is accompanied by increased body mass and rapid fat accretion and this aspect to have relation at least in part to increased insulin responsiveness in adipose tissue through CAP/Cbl pathway.
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spelling Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat dietMecanismos moleculares de indução de obesidade e resistência à insulina em animais destreinados submetidos a uma dieta rica em lipídes.Destreinamento. Obesidade. Resistência à insulina.The termination of exercise training (detraining) results in rapid fat accretion, weight gain and insulin resistance in both humans and rats. There is evident relationship between physical inactivity and insulin resistance. Different mechanisms may be involved in insulin resistance in this animal model. The aim of this study was to investigate the PI3-kinase/Akt and CAP/Cbl signaling pathways both involved with insulin-stimulated glucose uptake in white adipose tissue in detrained and sedentary animals submitted a high-fat diet. Wistar rats were submitted to swimming training during 8 weeks. Next this period the animals stop the training and received a rich-fat diet. The proteins from the insulin signaling pathway were analyzed by immunoprecipitation and immunoblotting. The results demonstrated that detraining result in an increased body mass and rapid body fat accretion. This fact was associated with increases insulin responsiveness in adipose tissue through IRS/PI3-Kinase/Akt pathway in detrained rats fed with a rich-fat diet. In addition, the CAP/Cbl pathway in adipose tissue was more insulin responsives in the detrained animals feeding with a rich-fat diet than S-DHL animals. In conclusion, the cessation of exercise is accompanied by increased body mass and rapid fat accretion and this aspect to have relation at least in part to increased insulin responsiveness in adipose tissue through CAP/Cbl pathway.A cessação do treinamento físico (destreinamento) resulta em rápido acréscimo da massa adiposa, ganho de peso e resistência à insulina tanto em humanos quanto em animais. No entanto, os mecanismos moleculares envolvidos nesse processo permanecem desconhecidos. Diferentes proteínas intracelulares podem estar envolvidas no processo de aquisição de ganho de peso e diminuição na ação da insulina nesse modelo animal. Este estudo teve como objetivos investigar as vias PI 3-quinase/Akt e CAP/Cbl, ambas importantes na captação ou utilização de glicose estimulada por insulina no tecido muscular adiposo branco. Utilizou-se, ratos Wistar que foram submetidos a um protocolo de exercício de natação por 8 semanas. Posteriormente os animais foram destreinados e nesse mesmo período de cessamento do programa de exercício foi oferecida aos ratos uma dieta rica em lipídes. Para análise das proteínas de interesse, foi realizado o método de imunoblot e imunoprecipitação. Verifica-se, através dos resultados obtidos, que animais destreinados tem um ganho de peso e de gordura epididimal mais acentuado comparado a animais sedentários. Esse fato foi associado a uma maior responsividade à insulina no tecido adiposo através da via IRS/PI 3-quinase/Akt dos animais destreinados submetidos à dieta rica em lipídes. Além disso, verifica-se, que a via CAP/Cbl encontra-se mais responsiva à insulina no tecido adiposo de animais destreinados em relação aos outros grupos experimentais. Conclui-se, que o destreinamento físico é acompanhado por um rápido período de ganho de peso e de massa adiposa e essas adaptações deve-se no mínimo em parte pela maior responsividade à insulina da via CAP/Cbl em tecido adiposo branco.Universidade Estadual Paulista - Câmpus de Rio Claro2007-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionDocumento Avaliado por Paresapplication/pdfhttps://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/81410.5016/814Motriz. Journal of Physical Education. UNESP; V. 13, N. 2 Suplemento (2007); S019-S028Motriz Revista de Educação Física; V. 13, N. 2 Suplemento (2007); S019-S0281980-6574reponame:Motriz (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESPporhttps://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/814/751Pauli, José RodrigoEsper Cintra, DennysRochete Ropelle, EduardoTeodoro de Souza, CláudioContin Moraes, JulianaAbdalla Saad, Mário Joséinfo:eu-repo/semantics/openAccess2013-04-19T15:14:35Zoai:periodicos.rc.biblioteca.unesp.br:article/814Revistahttp://www.periodicos.rc.biblioteca.unesp.br/index.php/motrizPUBhttps://old.scielo.br/oai/scielo-oai.phpmotriz@rc.unesp.br||mauerber@rc.unesp.br||azanesco@rc.unesp.br1980-65741415-9805opendoar:2013-04-19T15:14:35Motriz (Online) - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet
Mecanismos moleculares de indução de obesidade e resistência à insulina em animais destreinados submetidos a uma dieta rica em lipídes.
title Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet
spellingShingle Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet
Pauli, José Rodrigo
Destreinamento. Obesidade. Resistência à insulina.
title_short Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet
title_full Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet
title_fullStr Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet
title_full_unstemmed Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet
title_sort Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet
author Pauli, José Rodrigo
author_facet Pauli, José Rodrigo
Esper Cintra, Dennys
Rochete Ropelle, Eduardo
Teodoro de Souza, Cláudio
Contin Moraes, Juliana
Abdalla Saad, Mário José
author_role author
author2 Esper Cintra, Dennys
Rochete Ropelle, Eduardo
Teodoro de Souza, Cláudio
Contin Moraes, Juliana
Abdalla Saad, Mário José
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pauli, José Rodrigo
Esper Cintra, Dennys
Rochete Ropelle, Eduardo
Teodoro de Souza, Cláudio
Contin Moraes, Juliana
Abdalla Saad, Mário José
dc.subject.por.fl_str_mv Destreinamento. Obesidade. Resistência à insulina.
topic Destreinamento. Obesidade. Resistência à insulina.
description The termination of exercise training (detraining) results in rapid fat accretion, weight gain and insulin resistance in both humans and rats. There is evident relationship between physical inactivity and insulin resistance. Different mechanisms may be involved in insulin resistance in this animal model. The aim of this study was to investigate the PI3-kinase/Akt and CAP/Cbl signaling pathways both involved with insulin-stimulated glucose uptake in white adipose tissue in detrained and sedentary animals submitted a high-fat diet. Wistar rats were submitted to swimming training during 8 weeks. Next this period the animals stop the training and received a rich-fat diet. The proteins from the insulin signaling pathway were analyzed by immunoprecipitation and immunoblotting. The results demonstrated that detraining result in an increased body mass and rapid body fat accretion. This fact was associated with increases insulin responsiveness in adipose tissue through IRS/PI3-Kinase/Akt pathway in detrained rats fed with a rich-fat diet. In addition, the CAP/Cbl pathway in adipose tissue was more insulin responsives in the detrained animals feeding with a rich-fat diet than S-DHL animals. In conclusion, the cessation of exercise is accompanied by increased body mass and rapid fat accretion and this aspect to have relation at least in part to increased insulin responsiveness in adipose tissue through CAP/Cbl pathway.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Documento Avaliado por Pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/814
10.5016/814
url https://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/814
identifier_str_mv 10.5016/814
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.periodicos.rc.biblioteca.unesp.br/index.php/motriz/article/view/814/751
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista - Câmpus de Rio Claro
publisher.none.fl_str_mv Universidade Estadual Paulista - Câmpus de Rio Claro
dc.source.none.fl_str_mv Motriz. Journal of Physical Education. UNESP; V. 13, N. 2 Suplemento (2007); S019-S028
Motriz Revista de Educação Física; V. 13, N. 2 Suplemento (2007); S019-S028
1980-6574
reponame:Motriz (Online)
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Motriz (Online)
collection Motriz (Online)
repository.name.fl_str_mv Motriz (Online) - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv motriz@rc.unesp.br||mauerber@rc.unesp.br||azanesco@rc.unesp.br
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