Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Science |
Texto Completo: | https://ojs.ict.unesp.br/index.php/cob/article/view/1489 |
Resumo: | Objective: The aim of this study was to evaluate the effect of ozonized oil (OZ) on the oral levels of Candida spp. in patients with denture stomatitis. Material and Methods: In vitro tests were performed to validate antifungal activity and to standardize OZ conditions. Antifungal activity was screened against C. albicans and five non-albicans species (C. tropicalis, C. dubliniensis, C. krusei, C. guilliermondii, and C. parapsilosis). Also, the effects on C. albicans planktonic and biofilm were evaluated. After validation, OZ was included in a therapeutic protocol of denture stomatitis in vivo. Thirty patients used OZ and 20 used sodium bicarbonate (SB) for 14 days. After 7 and 14 days, clinical evaluation, isolation and identification of yeasts were performed. Isolates were identified by phenotypic and genotypic tests. Ozonized oil showed in vitro antifungal activity against all species of Candida. Ozonized oil reduced the number of viable cells in C. albicans biofilms. Oral candidal levels were lower in relation to baseline both after after 14 days of treatment with SB and OZ. Results: A total of 493 Candida spp. isolates was obtained and 80% were identified as C. albicans. Remission of denture stomatitis was observed in all patients after 7 days of treatment in both groups. Conclusion: Within the limits of the study we can conclude that ozonized olive oil can be a new alternative for the control of biofilm in patients with denture stomatitis. KeywordsOzone; Candida; Antifungal Agents; Stomatitis; Denture. |
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Brazilian Dental Science |
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Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitisObjective: The aim of this study was to evaluate the effect of ozonized oil (OZ) on the oral levels of Candida spp. in patients with denture stomatitis. Material and Methods: In vitro tests were performed to validate antifungal activity and to standardize OZ conditions. Antifungal activity was screened against C. albicans and five non-albicans species (C. tropicalis, C. dubliniensis, C. krusei, C. guilliermondii, and C. parapsilosis). Also, the effects on C. albicans planktonic and biofilm were evaluated. After validation, OZ was included in a therapeutic protocol of denture stomatitis in vivo. Thirty patients used OZ and 20 used sodium bicarbonate (SB) for 14 days. After 7 and 14 days, clinical evaluation, isolation and identification of yeasts were performed. Isolates were identified by phenotypic and genotypic tests. Ozonized oil showed in vitro antifungal activity against all species of Candida. Ozonized oil reduced the number of viable cells in C. albicans biofilms. Oral candidal levels were lower in relation to baseline both after after 14 days of treatment with SB and OZ. Results: A total of 493 Candida spp. isolates was obtained and 80% were identified as C. albicans. Remission of denture stomatitis was observed in all patients after 7 days of treatment in both groups. Conclusion: Within the limits of the study we can conclude that ozonized olive oil can be a new alternative for the control of biofilm in patients with denture stomatitis. KeywordsOzone; Candida; Antifungal Agents; Stomatitis; Denture. Institute of Science and Technology of São José dos Campos2018-03-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttps://ojs.ict.unesp.br/index.php/cob/article/view/148910.14295/bds.2018.v21i1.1489Brazilian Dental Science; Vol. 21 No. 1 (2018): Jan. - Mar. / 2018 - Published March 2018; 111-118Brazilian Dental Science; v. 21 n. 1 (2018): Jan. - Mar. / 2018 - Published March 2018; 111-1182178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/1489/1225https://ojs.ict.unesp.br/index.php/cob/article/view/1489/3120https://ojs.ict.unesp.br/index.php/cob/article/view/1489/3121https://ojs.ict.unesp.br/index.php/cob/article/view/1489/3122Copyright (c) 2018 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessCrastechini, EricaKoga-Ito, Cristiane YumiMachado, Suzan de FátimaTeodoro, Guilherme RodriguesBack-Brito, Graziella NuernbergSangalli, JorgianaDias Almeida, Janete2020-01-28T12:10:07Zoai:ojs.pkp.sfu.ca:article/1489Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:30:14.500070Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true |
dc.title.none.fl_str_mv |
Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis |
title |
Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis |
spellingShingle |
Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis Crastechini, Erica |
title_short |
Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis |
title_full |
Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis |
title_fullStr |
Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis |
title_full_unstemmed |
Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis |
title_sort |
Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis |
author |
Crastechini, Erica |
author_facet |
Crastechini, Erica Koga-Ito, Cristiane Yumi Machado, Suzan de Fátima Teodoro, Guilherme Rodrigues Back-Brito, Graziella Nuernberg Sangalli, Jorgiana Dias Almeida, Janete |
author_role |
author |
author2 |
Koga-Ito, Cristiane Yumi Machado, Suzan de Fátima Teodoro, Guilherme Rodrigues Back-Brito, Graziella Nuernberg Sangalli, Jorgiana Dias Almeida, Janete |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Crastechini, Erica Koga-Ito, Cristiane Yumi Machado, Suzan de Fátima Teodoro, Guilherme Rodrigues Back-Brito, Graziella Nuernberg Sangalli, Jorgiana Dias Almeida, Janete |
description |
Objective: The aim of this study was to evaluate the effect of ozonized oil (OZ) on the oral levels of Candida spp. in patients with denture stomatitis. Material and Methods: In vitro tests were performed to validate antifungal activity and to standardize OZ conditions. Antifungal activity was screened against C. albicans and five non-albicans species (C. tropicalis, C. dubliniensis, C. krusei, C. guilliermondii, and C. parapsilosis). Also, the effects on C. albicans planktonic and biofilm were evaluated. After validation, OZ was included in a therapeutic protocol of denture stomatitis in vivo. Thirty patients used OZ and 20 used sodium bicarbonate (SB) for 14 days. After 7 and 14 days, clinical evaluation, isolation and identification of yeasts were performed. Isolates were identified by phenotypic and genotypic tests. Ozonized oil showed in vitro antifungal activity against all species of Candida. Ozonized oil reduced the number of viable cells in C. albicans biofilms. Oral candidal levels were lower in relation to baseline both after after 14 days of treatment with SB and OZ. Results: A total of 493 Candida spp. isolates was obtained and 80% were identified as C. albicans. Remission of denture stomatitis was observed in all patients after 7 days of treatment in both groups. Conclusion: Within the limits of the study we can conclude that ozonized olive oil can be a new alternative for the control of biofilm in patients with denture stomatitis. KeywordsOzone; Candida; Antifungal Agents; Stomatitis; Denture. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1489 10.14295/bds.2018.v21i1.1489 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/1489 |
identifier_str_mv |
10.14295/bds.2018.v21i1.1489 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1489/1225 https://ojs.ict.unesp.br/index.php/cob/article/view/1489/3120 https://ojs.ict.unesp.br/index.php/cob/article/view/1489/3121 https://ojs.ict.unesp.br/index.php/cob/article/view/1489/3122 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Dental Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Dental Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document application/vnd.openxmlformats-officedocument.wordprocessingml.document application/vnd.openxmlformats-officedocument.wordprocessingml.document |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 21 No. 1 (2018): Jan. - Mar. / 2018 - Published March 2018; 111-118 Brazilian Dental Science; v. 21 n. 1 (2018): Jan. - Mar. / 2018 - Published March 2018; 111-118 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
_version_ |
1788346900222574592 |