Effects of ovariectomy, estrogen and soy isoflavones in rats submandibular glands
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Science |
Texto Completo: | https://ojs.ict.unesp.br/index.php/cob/article/view/1443 |
Resumo: | Objective: A decrease in granular convoluted tubule (GCT) cells and acini occurs in the submandibular glands of castrated female rats, while in rats submitted to hormone replacement and phytotherapy with soy isoflavones, this effect is reversed. This study aimed to elucidate the mechanisms through which these changes occur. Material and Methods: Rats (n=84) were ovariectomized and 21 were sham-operated. Ovariectomized rats were randomly subdivided and orally administered the following: 17 ?-estradiol (OVX-E; n=21), 15 mg/kg/day of soy isoflavone extract (OVX-I; n=21), 17 ?-estradiol + soy isoflavone extract (OVX-A; n=21); and water as placebo (OVX; n=21). The rats were euthanized three, five and eight weeks after ovariectomy. The submandibular salivary glands were submitted to histological processing with HE stain and immunohistochemistry was performed using the streptavidin-biotin-peroxidase complex. The cell area and the expression of proliferating cell nuclear antigen and estrogen receptor ? were evaluated. Results: The results were statistically analyzed by ANOVA and Tukey test. A decrease in the area of GCT cells in the OVX, was observed, in contrast with an increase in the OVX-E. PCNA in the acinar cells and estrogen receptors were elevated in the OVX-I group. Conclusion: Castration exerts an immediate reductive effect on the volume of GCT cells. Estrogens, soy isoflavones and their combination have different mechanisms of action on the homeostasis of the gland. Estrogens cause an increase in GCT cells area, while isoflavones enhance cell proliferation and the expression of estrogen receptor-?. Their association showed no additional increase in the effect studied.KeywordsEstrogen; Morphometry; Salivary glands; Soy isoflavones. |
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oai:ojs.pkp.sfu.ca:article/1443 |
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UNESP-20 |
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Brazilian Dental Science |
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Effects of ovariectomy, estrogen and soy isoflavones in rats submandibular glandsObjective: A decrease in granular convoluted tubule (GCT) cells and acini occurs in the submandibular glands of castrated female rats, while in rats submitted to hormone replacement and phytotherapy with soy isoflavones, this effect is reversed. This study aimed to elucidate the mechanisms through which these changes occur. Material and Methods: Rats (n=84) were ovariectomized and 21 were sham-operated. Ovariectomized rats were randomly subdivided and orally administered the following: 17 ?-estradiol (OVX-E; n=21), 15 mg/kg/day of soy isoflavone extract (OVX-I; n=21), 17 ?-estradiol + soy isoflavone extract (OVX-A; n=21); and water as placebo (OVX; n=21). The rats were euthanized three, five and eight weeks after ovariectomy. The submandibular salivary glands were submitted to histological processing with HE stain and immunohistochemistry was performed using the streptavidin-biotin-peroxidase complex. The cell area and the expression of proliferating cell nuclear antigen and estrogen receptor ? were evaluated. Results: The results were statistically analyzed by ANOVA and Tukey test. A decrease in the area of GCT cells in the OVX, was observed, in contrast with an increase in the OVX-E. PCNA in the acinar cells and estrogen receptors were elevated in the OVX-I group. Conclusion: Castration exerts an immediate reductive effect on the volume of GCT cells. Estrogens, soy isoflavones and their combination have different mechanisms of action on the homeostasis of the gland. Estrogens cause an increase in GCT cells area, while isoflavones enhance cell proliferation and the expression of estrogen receptor-?. Their association showed no additional increase in the effect studied.KeywordsEstrogen; Morphometry; Salivary glands; Soy isoflavones.Institute of Science and Technology of São José dos Campos2017-09-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttps://ojs.ict.unesp.br/index.php/cob/article/view/144310.14295/bds.2017.v20i3.1443Brazilian Dental Science; Vol. 20 No. 3 (2017): Jul. - Sep. / 2017 - Published September 2017; 110-125Brazilian Dental Science; v. 20 n. 3 (2017): Jul. - Sep. / 2017 - Published September 2017; 110-1252178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/1443/1175https://ojs.ict.unesp.br/index.php/cob/article/view/1443/3028https://ojs.ict.unesp.br/index.php/cob/article/view/1443/3040Copyright (c) 2017 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessVasconcellos, Luana Marotta ReisSilveira, Vanessa Ávila SarmentoMedeiros, Raphaela SilveiraAmorin, Márian YaktinCarvalho, Yasmin RodartePrado, Renata Falchete2020-01-28T12:13:41Zoai:ojs.pkp.sfu.ca:article/1443Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:30:13.246908Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true |
dc.title.none.fl_str_mv |
Effects of ovariectomy, estrogen and soy isoflavones in rats submandibular glands |
title |
Effects of ovariectomy, estrogen and soy isoflavones in rats submandibular glands |
spellingShingle |
Effects of ovariectomy, estrogen and soy isoflavones in rats submandibular glands Vasconcellos, Luana Marotta Reis |
title_short |
Effects of ovariectomy, estrogen and soy isoflavones in rats submandibular glands |
title_full |
Effects of ovariectomy, estrogen and soy isoflavones in rats submandibular glands |
title_fullStr |
Effects of ovariectomy, estrogen and soy isoflavones in rats submandibular glands |
title_full_unstemmed |
Effects of ovariectomy, estrogen and soy isoflavones in rats submandibular glands |
title_sort |
Effects of ovariectomy, estrogen and soy isoflavones in rats submandibular glands |
author |
Vasconcellos, Luana Marotta Reis |
author_facet |
Vasconcellos, Luana Marotta Reis Silveira, Vanessa Ávila Sarmento Medeiros, Raphaela Silveira Amorin, Márian Yaktin Carvalho, Yasmin Rodarte Prado, Renata Falchete |
author_role |
author |
author2 |
Silveira, Vanessa Ávila Sarmento Medeiros, Raphaela Silveira Amorin, Márian Yaktin Carvalho, Yasmin Rodarte Prado, Renata Falchete |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Vasconcellos, Luana Marotta Reis Silveira, Vanessa Ávila Sarmento Medeiros, Raphaela Silveira Amorin, Márian Yaktin Carvalho, Yasmin Rodarte Prado, Renata Falchete |
description |
Objective: A decrease in granular convoluted tubule (GCT) cells and acini occurs in the submandibular glands of castrated female rats, while in rats submitted to hormone replacement and phytotherapy with soy isoflavones, this effect is reversed. This study aimed to elucidate the mechanisms through which these changes occur. Material and Methods: Rats (n=84) were ovariectomized and 21 were sham-operated. Ovariectomized rats were randomly subdivided and orally administered the following: 17 ?-estradiol (OVX-E; n=21), 15 mg/kg/day of soy isoflavone extract (OVX-I; n=21), 17 ?-estradiol + soy isoflavone extract (OVX-A; n=21); and water as placebo (OVX; n=21). The rats were euthanized three, five and eight weeks after ovariectomy. The submandibular salivary glands were submitted to histological processing with HE stain and immunohistochemistry was performed using the streptavidin-biotin-peroxidase complex. The cell area and the expression of proliferating cell nuclear antigen and estrogen receptor ? were evaluated. Results: The results were statistically analyzed by ANOVA and Tukey test. A decrease in the area of GCT cells in the OVX, was observed, in contrast with an increase in the OVX-E. PCNA in the acinar cells and estrogen receptors were elevated in the OVX-I group. Conclusion: Castration exerts an immediate reductive effect on the volume of GCT cells. Estrogens, soy isoflavones and their combination have different mechanisms of action on the homeostasis of the gland. Estrogens cause an increase in GCT cells area, while isoflavones enhance cell proliferation and the expression of estrogen receptor-?. Their association showed no additional increase in the effect studied.KeywordsEstrogen; Morphometry; Salivary glands; Soy isoflavones. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1443 10.14295/bds.2017.v20i3.1443 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/1443 |
identifier_str_mv |
10.14295/bds.2017.v20i3.1443 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1443/1175 https://ojs.ict.unesp.br/index.php/cob/article/view/1443/3028 https://ojs.ict.unesp.br/index.php/cob/article/view/1443/3040 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Brazilian Dental Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Brazilian Dental Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 20 No. 3 (2017): Jul. - Sep. / 2017 - Published September 2017; 110-125 Brazilian Dental Science; v. 20 n. 3 (2017): Jul. - Sep. / 2017 - Published September 2017; 110-125 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
_version_ |
1788346900152320000 |