Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Science |
Texto Completo: | https://ojs.ict.unesp.br/index.php/cob/article/view/2366 |
Resumo: | ABSTRACT Background: Oral submucous fibrosis (OSMF) is graded according to various histological factors which include the epithelial changes and the connective tissue changes. These features could be identified in routine Hematoxylin and Eosin (H and E) staining in shades of pink. However, the use of special stains provides a contrast to various connective tissue components thereby aiding in better visualization of these connective tissue changes in advanced OSMF cases. Objective: To compare and evaluate muscle involvement and degeneration in advanced oral submucous fibrosis using three different stains namely, H&E, Van Gieson, and Masson’s Trichrome. Material and Methods: 10 Formalin-fixed paraffin-embedded tissue sections of advanced OSMFwere stained using 3 different stains namely Hematoxylin and Eosin (H&E), Van-Gieson, and Masson trichrome. The results obtained were tabulated and statistically analyzed using Kruskal Wallis ANOVA. Results: The hyalinization and fibrosis involving the skeletal muscle were better visualized in Masson’s Trichrome but were not statistically significant. The muscle degeneration in deeper areas was better visualized in Masson’s trichrome when compared to the H&E and Van Gieson. Conclusion: Hematoxylin and eosin stains all the connective tissue components in various shades of pink, use of special stains bestows contrast between different components of connective tissue, thus improvising grading of OSMF. Masson’s trichrome stain can be used as a single adjunct to hematoxylin and eosin stain as changes in the superficial and deeper connective tissue could be ascertained. Keywords Fibrosis; Masson’s trichrome stain; Muscle degeneration; Oral submucous fibrosis grading. |
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Brazilian Dental Science |
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Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional studyABSTRACT Background: Oral submucous fibrosis (OSMF) is graded according to various histological factors which include the epithelial changes and the connective tissue changes. These features could be identified in routine Hematoxylin and Eosin (H and E) staining in shades of pink. However, the use of special stains provides a contrast to various connective tissue components thereby aiding in better visualization of these connective tissue changes in advanced OSMF cases. Objective: To compare and evaluate muscle involvement and degeneration in advanced oral submucous fibrosis using three different stains namely, H&E, Van Gieson, and Masson’s Trichrome. Material and Methods: 10 Formalin-fixed paraffin-embedded tissue sections of advanced OSMFwere stained using 3 different stains namely Hematoxylin and Eosin (H&E), Van-Gieson, and Masson trichrome. The results obtained were tabulated and statistically analyzed using Kruskal Wallis ANOVA. Results: The hyalinization and fibrosis involving the skeletal muscle were better visualized in Masson’s Trichrome but were not statistically significant. The muscle degeneration in deeper areas was better visualized in Masson’s trichrome when compared to the H&E and Van Gieson. Conclusion: Hematoxylin and eosin stains all the connective tissue components in various shades of pink, use of special stains bestows contrast between different components of connective tissue, thus improvising grading of OSMF. Masson’s trichrome stain can be used as a single adjunct to hematoxylin and eosin stain as changes in the superficial and deeper connective tissue could be ascertained. Keywords Fibrosis; Masson’s trichrome stain; Muscle degeneration; Oral submucous fibrosis grading.Institute of Science and Technology of São José dos Campos2021-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.ict.unesp.br/index.php/cob/article/view/236610.14295/bds.2021.v24i3.2366Brazilian Dental Science; Vol. 24 No. 3 (2021): Jul - Sep / 2021 - published Jul 2021Brazilian Dental Science; v. 24 n. 3 (2021): Jul - Sep / 2021 - published Jul 20212178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/2366/4314Copyright (c) 2021 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessUmashankar, KrishnapriyaJ Sherlin, Herald Jayaraj, Gifrina Ravi Don, Kanchi Santhanam, Archana 2021-08-06T18:15:05Zoai:ojs.pkp.sfu.ca:article/2366Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:30:34.796769Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true |
dc.title.none.fl_str_mv |
Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study |
title |
Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study |
spellingShingle |
Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study Umashankar, Krishnapriya |
title_short |
Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study |
title_full |
Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study |
title_fullStr |
Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study |
title_full_unstemmed |
Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study |
title_sort |
Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study |
author |
Umashankar, Krishnapriya |
author_facet |
Umashankar, Krishnapriya J Sherlin, Herald Jayaraj, Gifrina Ravi Don, Kanchi Santhanam, Archana |
author_role |
author |
author2 |
J Sherlin, Herald Jayaraj, Gifrina Ravi Don, Kanchi Santhanam, Archana |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Umashankar, Krishnapriya J Sherlin, Herald Jayaraj, Gifrina Ravi Don, Kanchi Santhanam, Archana |
description |
ABSTRACT Background: Oral submucous fibrosis (OSMF) is graded according to various histological factors which include the epithelial changes and the connective tissue changes. These features could be identified in routine Hematoxylin and Eosin (H and E) staining in shades of pink. However, the use of special stains provides a contrast to various connective tissue components thereby aiding in better visualization of these connective tissue changes in advanced OSMF cases. Objective: To compare and evaluate muscle involvement and degeneration in advanced oral submucous fibrosis using three different stains namely, H&E, Van Gieson, and Masson’s Trichrome. Material and Methods: 10 Formalin-fixed paraffin-embedded tissue sections of advanced OSMFwere stained using 3 different stains namely Hematoxylin and Eosin (H&E), Van-Gieson, and Masson trichrome. The results obtained were tabulated and statistically analyzed using Kruskal Wallis ANOVA. Results: The hyalinization and fibrosis involving the skeletal muscle were better visualized in Masson’s Trichrome but were not statistically significant. The muscle degeneration in deeper areas was better visualized in Masson’s trichrome when compared to the H&E and Van Gieson. Conclusion: Hematoxylin and eosin stains all the connective tissue components in various shades of pink, use of special stains bestows contrast between different components of connective tissue, thus improvising grading of OSMF. Masson’s trichrome stain can be used as a single adjunct to hematoxylin and eosin stain as changes in the superficial and deeper connective tissue could be ascertained. Keywords Fibrosis; Masson’s trichrome stain; Muscle degeneration; Oral submucous fibrosis grading. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/2366 10.14295/bds.2021.v24i3.2366 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/2366 |
identifier_str_mv |
10.14295/bds.2021.v24i3.2366 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/2366/4314 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Brazilian Dental Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Brazilian Dental Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 24 No. 3 (2021): Jul - Sep / 2021 - published Jul 2021 Brazilian Dental Science; v. 24 n. 3 (2021): Jul - Sep / 2021 - published Jul 2021 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
_version_ |
1788346901870936064 |