Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Science |
Texto Completo: | https://ojs.ict.unesp.br/index.php/cob/article/view/865 |
Resumo: | Purpose: The aim of this study was to compare several non-vital dental bleaching agents for their in vitro cytotoxicity to human gingival fibroblasts primary cell line.Methods: The cells were cultivated in DMEM and were seed in plates of 96 wells; then, it was exposed to the conditioned medium according to the experimental groups (n=12): G1- SP (sodium perborate) + distilled water; G2- SP + 20% CP (carbamide peroxide); G3- 20% CP; G4- SP + 35% HP (hydrogen peroxide); G5- 35% HP. In the control group (n=12), corresponded to the curve of cell growth and viability, the cells did not receive any treatment. Cell viability was measured photometrically using a MTT assay after a 24h and 48h of exposure period. Data were submitted to ANOVA and Tukey’s tests.Results: All the experimental groups presented high cytotoxicity statically in comparison to the control group. The rank of the most to the least toxic material after 24 hours was: SP + DW > 35% PH > PS + 20% PC > PS + 35% PH > 20% PC; and after 48 hours was: SP + DW > PS + 20% PC > 35% PH > PS + 35% PH > 20% PC. All the bleaching agents had presented cytotoxicity effects, reducing significantly the cell viability, however, in the conditions that the study was conducted the association of sodium perborate with distilled water was the most toxic bleaching agent. |
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Brazilian Dental Science |
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spelling |
Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblastsPurpose: The aim of this study was to compare several non-vital dental bleaching agents for their in vitro cytotoxicity to human gingival fibroblasts primary cell line.Methods: The cells were cultivated in DMEM and were seed in plates of 96 wells; then, it was exposed to the conditioned medium according to the experimental groups (n=12): G1- SP (sodium perborate) + distilled water; G2- SP + 20% CP (carbamide peroxide); G3- 20% CP; G4- SP + 35% HP (hydrogen peroxide); G5- 35% HP. In the control group (n=12), corresponded to the curve of cell growth and viability, the cells did not receive any treatment. Cell viability was measured photometrically using a MTT assay after a 24h and 48h of exposure period. Data were submitted to ANOVA and Tukey’s tests.Results: All the experimental groups presented high cytotoxicity statically in comparison to the control group. The rank of the most to the least toxic material after 24 hours was: SP + DW > 35% PH > PS + 20% PC > PS + 35% PH > 20% PC; and after 48 hours was: SP + DW > PS + 20% PC > 35% PH > PS + 35% PH > 20% PC. All the bleaching agents had presented cytotoxicity effects, reducing significantly the cell viability, however, in the conditions that the study was conducted the association of sodium perborate with distilled water was the most toxic bleaching agent.Institute of Science and Technology of São José dos Campos2013-03-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfimage/jpeghttps://ojs.ict.unesp.br/index.php/cob/article/view/86510.14295/bds.2013.v16i1.865Brazilian Dental Science; Vol. 16 No. 1 (2013): Jan. - Mar. / 2013 - Published March 2013; 59-65Brazilian Dental Science; v. 16 n. 1 (2013): Jan. - Mar. / 2013 - Published March 2013; 59-652178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/865/787https://ojs.ict.unesp.br/index.php/cob/article/view/865/1814Fernandes, Aletéia MassulaMarques, Marcia MartinsCamargo, Samira Esteves AfonsoCardoso, Paula ElaineCamargo, Carlos Henrique RibeiroValera, Marcia Carneiroinfo:eu-repo/semantics/openAccess2020-01-28T12:37:49Zoai:ojs.pkp.sfu.ca:article/865Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:29:52.711336Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true |
dc.title.none.fl_str_mv |
Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts |
title |
Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts |
spellingShingle |
Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts Fernandes, Aletéia Massula |
title_short |
Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts |
title_full |
Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts |
title_fullStr |
Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts |
title_full_unstemmed |
Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts |
title_sort |
Cytotoxicity of non-vital dental bleaching agents in human gingival fibroblasts |
author |
Fernandes, Aletéia Massula |
author_facet |
Fernandes, Aletéia Massula Marques, Marcia Martins Camargo, Samira Esteves Afonso Cardoso, Paula Elaine Camargo, Carlos Henrique Ribeiro Valera, Marcia Carneiro |
author_role |
author |
author2 |
Marques, Marcia Martins Camargo, Samira Esteves Afonso Cardoso, Paula Elaine Camargo, Carlos Henrique Ribeiro Valera, Marcia Carneiro |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Aletéia Massula Marques, Marcia Martins Camargo, Samira Esteves Afonso Cardoso, Paula Elaine Camargo, Carlos Henrique Ribeiro Valera, Marcia Carneiro |
description |
Purpose: The aim of this study was to compare several non-vital dental bleaching agents for their in vitro cytotoxicity to human gingival fibroblasts primary cell line.Methods: The cells were cultivated in DMEM and were seed in plates of 96 wells; then, it was exposed to the conditioned medium according to the experimental groups (n=12): G1- SP (sodium perborate) + distilled water; G2- SP + 20% CP (carbamide peroxide); G3- 20% CP; G4- SP + 35% HP (hydrogen peroxide); G5- 35% HP. In the control group (n=12), corresponded to the curve of cell growth and viability, the cells did not receive any treatment. Cell viability was measured photometrically using a MTT assay after a 24h and 48h of exposure period. Data were submitted to ANOVA and Tukey’s tests.Results: All the experimental groups presented high cytotoxicity statically in comparison to the control group. The rank of the most to the least toxic material after 24 hours was: SP + DW > 35% PH > PS + 20% PC > PS + 35% PH > 20% PC; and after 48 hours was: SP + DW > PS + 20% PC > 35% PH > PS + 35% PH > 20% PC. All the bleaching agents had presented cytotoxicity effects, reducing significantly the cell viability, however, in the conditions that the study was conducted the association of sodium perborate with distilled water was the most toxic bleaching agent. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/865 10.14295/bds.2013.v16i1.865 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/865 |
identifier_str_mv |
10.14295/bds.2013.v16i1.865 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/865/787 https://ojs.ict.unesp.br/index.php/cob/article/view/865/1814 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf image/jpeg |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 16 No. 1 (2013): Jan. - Mar. / 2013 - Published March 2013; 59-65 Brazilian Dental Science; v. 16 n. 1 (2013): Jan. - Mar. / 2013 - Published March 2013; 59-65 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
_version_ |
1788346898514444288 |