Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expression

Detalhes bibliográficos
Autor(a) principal: Silva, Michelle Peneluppi
Data de Publicação: 2019
Outros Autores: de Barros, Patrícia Pimentel, Jorjão, Adeline Lacerda, Rossoni, Rodnei Dennis, Junqueira, Juliana Campos, Jorge, Antonio Olavo Cardoso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Science
Texto Completo: https://ojs.ict.unesp.br/index.php/cob/article/view/1692
Resumo: Objective: The aim of this study was evaluate the effect of Bacillus subtilis on Candida albicans biofilm formation and filamentation by evaluating the gene expression of ALS3, HWP1, BCR1, EFG1 and TEC1. Material and Methods: Mixed (C. albicans / B.subtilis) and monotypic biofilms were cultured in plates at 37°C for 48 h under shaking for counting viable cells (CFU / mL) and analysis of gene expression by real-time PCR. The C. albicans filamentation assay was performed in medium containing 10% fetal bovine serum at 37°C for 6 hours. Data was analysed by t-Student and Mann– Whitney tests. Results: B. subtilis reduced the biofilm formation of C. albicans in 1 log when cultured in the same environment (p<0.0001). In addition, it significantly reduced the yeast -hypha transition affecting the morphology of C. albicans. Among all of the analyzed genes, the ALS3 and HWP1 genes were the most affected, achieving 111.1- and 333.3- fold decreases in the C. albicans biofilms associated with B. subtilis, respectively. Conclusion: B. subtilis reduced the biofilm formation and filamentation of C. albicans by negatively regulating the ALS3, HWP1, BCR1, EFG1 and TEC1 genes that are essential for the production of biofilm and hyphae.KeywordsBacillus subtilis; Candida albicans; Biofilm; Filamentation; Gene expression.
id UNESP-20_fa734cd5fd9fe0ab10bf72f772e90c2f
oai_identifier_str oai:ojs.pkp.sfu.ca:article/1692
network_acronym_str UNESP-20
network_name_str Brazilian Dental Science
repository_id_str
spelling Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expressionObjective: The aim of this study was evaluate the effect of Bacillus subtilis on Candida albicans biofilm formation and filamentation by evaluating the gene expression of ALS3, HWP1, BCR1, EFG1 and TEC1. Material and Methods: Mixed (C. albicans / B.subtilis) and monotypic biofilms were cultured in plates at 37°C for 48 h under shaking for counting viable cells (CFU / mL) and analysis of gene expression by real-time PCR. The C. albicans filamentation assay was performed in medium containing 10% fetal bovine serum at 37°C for 6 hours. Data was analysed by t-Student and Mann– Whitney tests. Results: B. subtilis reduced the biofilm formation of C. albicans in 1 log when cultured in the same environment (p<0.0001). In addition, it significantly reduced the yeast -hypha transition affecting the morphology of C. albicans. Among all of the analyzed genes, the ALS3 and HWP1 genes were the most affected, achieving 111.1- and 333.3- fold decreases in the C. albicans biofilms associated with B. subtilis, respectively. Conclusion: B. subtilis reduced the biofilm formation and filamentation of C. albicans by negatively regulating the ALS3, HWP1, BCR1, EFG1 and TEC1 genes that are essential for the production of biofilm and hyphae.KeywordsBacillus subtilis; Candida albicans; Biofilm; Filamentation; Gene expression.Institute of Science and Technology of São José dos Campos2019-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttps://ojs.ict.unesp.br/index.php/cob/article/view/169210.14295/bds.2019.v22i2.1692Brazilian Dental Science; Vol. 22 No. 2 (2019): Apr. - Jun. / 2019 - Published April 2019; 252-259Brazilian Dental Science; v. 22 n. 2 (2019): Apr. - Jun. / 2019 - Published April 2019; 252-2592178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/1692/1356https://ojs.ict.unesp.br/index.php/cob/article/view/1692/3577https://ojs.ict.unesp.br/index.php/cob/article/view/1692/3581Copyright (c) 2019 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessSilva, Michelle Peneluppide Barros, Patrícia PimentelJorjão, Adeline LacerdaRossoni, Rodnei DennisJunqueira, Juliana CamposJorge, Antonio Olavo Cardoso2020-01-28T11:57:54Zoai:ojs.pkp.sfu.ca:article/1692Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:30:20.539778Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true
dc.title.none.fl_str_mv Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expression
title Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expression
spellingShingle Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expression
Silva, Michelle Peneluppi
title_short Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expression
title_full Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expression
title_fullStr Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expression
title_full_unstemmed Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expression
title_sort Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expression
author Silva, Michelle Peneluppi
author_facet Silva, Michelle Peneluppi
de Barros, Patrícia Pimentel
Jorjão, Adeline Lacerda
Rossoni, Rodnei Dennis
Junqueira, Juliana Campos
Jorge, Antonio Olavo Cardoso
author_role author
author2 de Barros, Patrícia Pimentel
Jorjão, Adeline Lacerda
Rossoni, Rodnei Dennis
Junqueira, Juliana Campos
Jorge, Antonio Olavo Cardoso
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Michelle Peneluppi
de Barros, Patrícia Pimentel
Jorjão, Adeline Lacerda
Rossoni, Rodnei Dennis
Junqueira, Juliana Campos
Jorge, Antonio Olavo Cardoso
description Objective: The aim of this study was evaluate the effect of Bacillus subtilis on Candida albicans biofilm formation and filamentation by evaluating the gene expression of ALS3, HWP1, BCR1, EFG1 and TEC1. Material and Methods: Mixed (C. albicans / B.subtilis) and monotypic biofilms were cultured in plates at 37°C for 48 h under shaking for counting viable cells (CFU / mL) and analysis of gene expression by real-time PCR. The C. albicans filamentation assay was performed in medium containing 10% fetal bovine serum at 37°C for 6 hours. Data was analysed by t-Student and Mann– Whitney tests. Results: B. subtilis reduced the biofilm formation of C. albicans in 1 log when cultured in the same environment (p<0.0001). In addition, it significantly reduced the yeast -hypha transition affecting the morphology of C. albicans. Among all of the analyzed genes, the ALS3 and HWP1 genes were the most affected, achieving 111.1- and 333.3- fold decreases in the C. albicans biofilms associated with B. subtilis, respectively. Conclusion: B. subtilis reduced the biofilm formation and filamentation of C. albicans by negatively regulating the ALS3, HWP1, BCR1, EFG1 and TEC1 genes that are essential for the production of biofilm and hyphae.KeywordsBacillus subtilis; Candida albicans; Biofilm; Filamentation; Gene expression.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.ict.unesp.br/index.php/cob/article/view/1692
10.14295/bds.2019.v22i2.1692
url https://ojs.ict.unesp.br/index.php/cob/article/view/1692
identifier_str_mv 10.14295/bds.2019.v22i2.1692
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.ict.unesp.br/index.php/cob/article/view/1692/1356
https://ojs.ict.unesp.br/index.php/cob/article/view/1692/3577
https://ojs.ict.unesp.br/index.php/cob/article/view/1692/3581
dc.rights.driver.fl_str_mv Copyright (c) 2019 Brazilian Dental Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Brazilian Dental Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Institute of Science and Technology of São José dos Campos
publisher.none.fl_str_mv Institute of Science and Technology of São José dos Campos
dc.source.none.fl_str_mv Brazilian Dental Science; Vol. 22 No. 2 (2019): Apr. - Jun. / 2019 - Published April 2019; 252-259
Brazilian Dental Science; v. 22 n. 2 (2019): Apr. - Jun. / 2019 - Published April 2019; 252-259
2178-6011
reponame:Brazilian Dental Science
instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Brazilian Dental Science
collection Brazilian Dental Science
repository.name.fl_str_mv Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
repository.mail.fl_str_mv sergio@fosjc.unesp.br||sergio@fosjc.unesp.br
_version_ 1788346900772028416