Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

• Autor(a) principal: Victório,Cristiane P.
• Data de Publicação: 2009
• Outros Autores: Lage,Celso Luiz S., Kuster,Ricardo M.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Eclética Química
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702009000100003
• topic: high performance liquid chromatography; maceration; microwave; ultrasonic; Zingiberaceae

The current study aims to verify the best method for a rapid and efficient extraction of flavonoids from Alpinia zerumbet. Dried leaves were extracted using distillated water and ethanol 70% by extraction methods of shaking maceration, ultrasonic, microwave and stirring. By the application of TLC and reversed-phase HPLC techniques the rutin and kaempferol-3-O-glucuronide were detected. Ethanol 70% was more efficient for flavonoids extraction than water. No significant yielding variation was verified for ultrasonic, microwave and stirring methods using ethanol 70% (11 to 14%). The relative concentration of rutin and kaempferol-3-O-glucuronide, respectively, was higher by ultrasonic (1.5 and 5.62 mg g-1 dried leaves, respectively) and by microwave (1.0 and 6.64 mg g-1 dried leaves) methods using ethanol. Rapid and simplified extraction proceeding optimize phytochemical work and acquisition of secondary metabolites.