An Evaluation of the Quality of the Desinfection Process in Inanimated Surfaces of Basic Health Units by Biomarkers Research
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Brasileira Multidisciplinar |
Texto Completo: | http://revistarebram.com/index.php/revistauniara/article/view/55 |
Resumo: | Infection Related Health Care – IRHC may occur by exogenous transmission through the contamination of contaminated surfaces. This study aimed at verifying the quality of the process of disinfecting inanimate surfaces of Basic Health Units – BHU in a northeastern city in São Paulo state, through the presence of biomarkers, Staphylococcus aureus and Escherichia coli. We evaluated 7 UBS in random times and days, covering the following areas: dressing-room doorknob, drinking fountains and faucets, office desk, reception counter. Sterile swabs were rubbed on a 20 cm2 surface and transported to the laboratory in Stuart medium to the Clinical Analyses Didactic Laboratory of UNIFEV. The samples were cultured on Blood agar and MacConkey agar at 35 ± 1oC for 24 hours in aerobic and microaerophilic jar, respectively. Staphylococcus aureus was identified by the production of hemolysin, catalase and coagulase. Escherichia coli was identified using the biochemical tests: TSI, citrate, urease, indole, lysine, ornithine and arginine. Of the 105 samples analyzed, 6.66% of the samples were positive for Staphylococcus aureus and Escherichia coli to 2.85%. The Areas which showed the presence of biomarkers were: the reception booth, booth pharmacy, handles of the dressing room, dressing room faucet and drinking fountain. These results corroborate other studies that show that inanimate surfaces are important sources of contamination in the healthcare environment, contributing to crosscontamination and, consequently, to the increase of infection to the patient who is subjected to procedures in this environment. Within this context, government, by means of public health policies, is responsible for the training of health professionals, contributing to the promotion and prevention of public health |
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An Evaluation of the Quality of the Desinfection Process in Inanimated Surfaces of Basic Health Units by Biomarkers ResearchAvaliação da Qualidade do Processo de Desinfecção em Superfícies Inanimadas de Unidades Básicas de Saúde por Pesquisa de BiomarcadoresStaphylococcus aureusEscherichia coliSurfacesIRHABiomarker.Staphylococcus aureusEscherichia coliSuperfíciesIRASBiomarcadores.Infection Related Health Care – IRHC may occur by exogenous transmission through the contamination of contaminated surfaces. This study aimed at verifying the quality of the process of disinfecting inanimate surfaces of Basic Health Units – BHU in a northeastern city in São Paulo state, through the presence of biomarkers, Staphylococcus aureus and Escherichia coli. We evaluated 7 UBS in random times and days, covering the following areas: dressing-room doorknob, drinking fountains and faucets, office desk, reception counter. Sterile swabs were rubbed on a 20 cm2 surface and transported to the laboratory in Stuart medium to the Clinical Analyses Didactic Laboratory of UNIFEV. The samples were cultured on Blood agar and MacConkey agar at 35 ± 1oC for 24 hours in aerobic and microaerophilic jar, respectively. Staphylococcus aureus was identified by the production of hemolysin, catalase and coagulase. Escherichia coli was identified using the biochemical tests: TSI, citrate, urease, indole, lysine, ornithine and arginine. Of the 105 samples analyzed, 6.66% of the samples were positive for Staphylococcus aureus and Escherichia coli to 2.85%. The Areas which showed the presence of biomarkers were: the reception booth, booth pharmacy, handles of the dressing room, dressing room faucet and drinking fountain. These results corroborate other studies that show that inanimate surfaces are important sources of contamination in the healthcare environment, contributing to crosscontamination and, consequently, to the increase of infection to the patient who is subjected to procedures in this environment. Within this context, government, by means of public health policies, is responsible for the training of health professionals, contributing to the promotion and prevention of public healthA Infecção Relacionada à Assistência à Saúde – IRAS pode ocorrer pela transmissão exógena por meio da contaminação de superfícies contaminadas. A presente pesquisa teve como objetivo verificar a qualidade do processo de desinfecção de superfícies inanimadas de Unidades Básicas de Saúde – UBS de um município do noroeste paulista, através da presença de Staphylococcus aureus e Escherichia coli. Foram avaliadas sete UBSs, em horários e dias aleatórios, abrangendo as seguintes superfícies: maçaneta da sala de curativos, torneiras e bebedouros, mesa do consultório, guichê da recepção. Swabs estéreis foram friccionados em 20 cm2 das superfícies e transportados em meio Stuart ao Laboratório Didático de Análises Clínicas do UNIFEV. As amostras foram cultivadas em Ágar Sangue e MacConkey, a 35±1oC por 24 horas, em jarra de microaerofilia e aerobiose, respectivamente. O Staphylococcus aureus foi identificado por meio da produção de hemolysin, catalalase and coagulase. Já a Escherichia coli, via os seguintes testes bioquímicos: TSI,citrato urease, indole, lysine, ornithine and arginina. Das 105 amostras analisadas, 6,66% apresentaram positivas para S. aureus e 2,85% para E. coli. As superfícies que apresentaram a presença dos bioindicadores foram: o guichê da recepção, guichê da farmácia, maçaneta da sala de curativos, torneira da sala de curativos e bebedouro. Esses resultados corroboram outros estudos que demonstram que as superfícies inanimadas são importantes fontes de contaminação no ambiente de assistência à saúde, contribuindo com a contaminação cruzada e, consequentemente, com o aumento de infecção ao paciente que está sujeito aos procedimentos nesse contexto. Dentro desse contexto, o poder público, através das políticas públicas de saúde, é responsável pela capacitação dos profissionais da saúde, contribuindo na promoção e prevenção da saúde coletiva.Revista Brasileira Multidisciplinar - ReBraM2013-01-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistarebram.com/index.php/revistauniara/article/view/5510.25061/2527-2675/ReBraM/2013.v16i1.55Revista Brasileira Multidisciplinar - ReBraM; v. 16 n. 1 (2013): janeiro/junho; 183-1902527-26751415-3580reponame:Revista Brasileira Multidisciplinarinstname:Universidade de Araraquara (UNIARA)instacron:UNIARAporhttp://revistarebram.com/index.php/revistauniara/article/view/55/37Fucci, Ana Paula BandeiraMarcolino, Moniele Stortide Castro, Valéria da Cruz Oliveirainfo:eu-repo/semantics/openAccess2018-03-08T14:18:36Zoai:ojs.revistarebram.com:article/55Revistahttp://revistarebram.com/index.php/revistauniaraPRIhttps://revistarebram.com/index.php/revistauniara/oairevistauniara@uniara.com||2527-26752527-2675opendoar:2018-03-08T14:18:36Revista Brasileira Multidisciplinar - Universidade de Araraquara (UNIARA)false |
dc.title.none.fl_str_mv |
An Evaluation of the Quality of the Desinfection Process in Inanimated Surfaces of Basic Health Units by Biomarkers Research Avaliação da Qualidade do Processo de Desinfecção em Superfícies Inanimadas de Unidades Básicas de Saúde por Pesquisa de Biomarcadores |
title |
An Evaluation of the Quality of the Desinfection Process in Inanimated Surfaces of Basic Health Units by Biomarkers Research |
spellingShingle |
An Evaluation of the Quality of the Desinfection Process in Inanimated Surfaces of Basic Health Units by Biomarkers Research Fucci, Ana Paula Bandeira Staphylococcus aureus Escherichia coli Surfaces IRHA Biomarker. Staphylococcus aureus Escherichia coli Superfícies IRAS Biomarcadores. |
title_short |
An Evaluation of the Quality of the Desinfection Process in Inanimated Surfaces of Basic Health Units by Biomarkers Research |
title_full |
An Evaluation of the Quality of the Desinfection Process in Inanimated Surfaces of Basic Health Units by Biomarkers Research |
title_fullStr |
An Evaluation of the Quality of the Desinfection Process in Inanimated Surfaces of Basic Health Units by Biomarkers Research |
title_full_unstemmed |
An Evaluation of the Quality of the Desinfection Process in Inanimated Surfaces of Basic Health Units by Biomarkers Research |
title_sort |
An Evaluation of the Quality of the Desinfection Process in Inanimated Surfaces of Basic Health Units by Biomarkers Research |
author |
Fucci, Ana Paula Bandeira |
author_facet |
Fucci, Ana Paula Bandeira Marcolino, Moniele Storti de Castro, Valéria da Cruz Oliveira |
author_role |
author |
author2 |
Marcolino, Moniele Storti de Castro, Valéria da Cruz Oliveira |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Fucci, Ana Paula Bandeira Marcolino, Moniele Storti de Castro, Valéria da Cruz Oliveira |
dc.subject.por.fl_str_mv |
Staphylococcus aureus Escherichia coli Surfaces IRHA Biomarker. Staphylococcus aureus Escherichia coli Superfícies IRAS Biomarcadores. |
topic |
Staphylococcus aureus Escherichia coli Surfaces IRHA Biomarker. Staphylococcus aureus Escherichia coli Superfícies IRAS Biomarcadores. |
description |
Infection Related Health Care – IRHC may occur by exogenous transmission through the contamination of contaminated surfaces. This study aimed at verifying the quality of the process of disinfecting inanimate surfaces of Basic Health Units – BHU in a northeastern city in São Paulo state, through the presence of biomarkers, Staphylococcus aureus and Escherichia coli. We evaluated 7 UBS in random times and days, covering the following areas: dressing-room doorknob, drinking fountains and faucets, office desk, reception counter. Sterile swabs were rubbed on a 20 cm2 surface and transported to the laboratory in Stuart medium to the Clinical Analyses Didactic Laboratory of UNIFEV. The samples were cultured on Blood agar and MacConkey agar at 35 ± 1oC for 24 hours in aerobic and microaerophilic jar, respectively. Staphylococcus aureus was identified by the production of hemolysin, catalase and coagulase. Escherichia coli was identified using the biochemical tests: TSI, citrate, urease, indole, lysine, ornithine and arginine. Of the 105 samples analyzed, 6.66% of the samples were positive for Staphylococcus aureus and Escherichia coli to 2.85%. The Areas which showed the presence of biomarkers were: the reception booth, booth pharmacy, handles of the dressing room, dressing room faucet and drinking fountain. These results corroborate other studies that show that inanimate surfaces are important sources of contamination in the healthcare environment, contributing to crosscontamination and, consequently, to the increase of infection to the patient who is subjected to procedures in this environment. Within this context, government, by means of public health policies, is responsible for the training of health professionals, contributing to the promotion and prevention of public health |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://revistarebram.com/index.php/revistauniara/article/view/55 10.25061/2527-2675/ReBraM/2013.v16i1.55 |
url |
http://revistarebram.com/index.php/revistauniara/article/view/55 |
identifier_str_mv |
10.25061/2527-2675/ReBraM/2013.v16i1.55 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://revistarebram.com/index.php/revistauniara/article/view/55/37 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Revista Brasileira Multidisciplinar - ReBraM |
publisher.none.fl_str_mv |
Revista Brasileira Multidisciplinar - ReBraM |
dc.source.none.fl_str_mv |
Revista Brasileira Multidisciplinar - ReBraM; v. 16 n. 1 (2013): janeiro/junho; 183-190 2527-2675 1415-3580 reponame:Revista Brasileira Multidisciplinar instname:Universidade de Araraquara (UNIARA) instacron:UNIARA |
instname_str |
Universidade de Araraquara (UNIARA) |
instacron_str |
UNIARA |
institution |
UNIARA |
reponame_str |
Revista Brasileira Multidisciplinar |
collection |
Revista Brasileira Multidisciplinar |
repository.name.fl_str_mv |
Revista Brasileira Multidisciplinar - Universidade de Araraquara (UNIARA) |
repository.mail.fl_str_mv |
revistauniara@uniara.com|| |
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1797174604535431168 |