Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study

Detalhes bibliográficos
Autor(a) principal: Lemos, Janaína Cavalcante Lemos
Data de Publicação: 2015
Outros Autores: Gurgel, Bruno César de Vasconcelos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian journal of oral sciences (Online)
Texto Completo: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640822
Resumo: Aim: To evaluate the involvement of Th2 cells in different periods of the active phase of experimental periodontal disease and expression of the R1 subunit of the receptor for IFN-γ during the early and advanced progression of the disease. Methods: Experimental periodontitis was induced in 30 male Wistar rats by placing cotton ligatures around the mandibular first molars. The rats were then randomly assigned into two groups: G1=15 and G2=15, in group G1, ligatures were maintained for 2 days, a period that corresponds to the initial stage of periodontal disease in rats, in G2 ligatures were left for 15 days, a period that corresponds to the advanced stage of periodontal disease. The contra-lateral teeth served as controls (unligated). An immunohistochemical investigation of the gingival tissue was performed to detect the presence of the Th2 specific transcription factor (GATA3). Results: Light microscopy analysis revealed a decreased expression of GATA-3-positive cells when bone loss progressed. IFN-γ R1 was detected at an early stage during the active phase of disease, but the expression of positive cells remained unaltered during the remaining period of the study. Conclusions: These results indicate that the Th2 response may have a protective role during the pathogenesis of experimental periodontal disease, and that the IFN-γ R1 subunit may not be associated with periodontal disease progression.
id UNICAMP-8_239db56ecd6fe90f80b0b4255b7eb2d8
oai_identifier_str oai:ojs.periodicos.sbu.unicamp.br:article/8640822
network_acronym_str UNICAMP-8
network_name_str Brazilian journal of oral sciences (Online)
repository_id_str
spelling Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical studyRatsPeriodontal diseasesTh1 cellsTh2 cellsCytokinesImmunohistochemistryOdontologiaPeriodontologiaAim: To evaluate the involvement of Th2 cells in different periods of the active phase of experimental periodontal disease and expression of the R1 subunit of the receptor for IFN-γ during the early and advanced progression of the disease. Methods: Experimental periodontitis was induced in 30 male Wistar rats by placing cotton ligatures around the mandibular first molars. The rats were then randomly assigned into two groups: G1=15 and G2=15, in group G1, ligatures were maintained for 2 days, a period that corresponds to the initial stage of periodontal disease in rats, in G2 ligatures were left for 15 days, a period that corresponds to the advanced stage of periodontal disease. The contra-lateral teeth served as controls (unligated). An immunohistochemical investigation of the gingival tissue was performed to detect the presence of the Th2 specific transcription factor (GATA3). Results: Light microscopy analysis revealed a decreased expression of GATA-3-positive cells when bone loss progressed. IFN-γ R1 was detected at an early stage during the active phase of disease, but the expression of positive cells remained unaltered during the remaining period of the study. Conclusions: These results indicate that the Th2 response may have a protective role during the pathogenesis of experimental periodontal disease, and that the IFN-γ R1 subunit may not be associated with periodontal disease progression.Universidade Estadual de Campinas2015-05-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640822Brazilian Journal of Oral Sciences; v. 14 n. 2 (2015): Apr./Jun.; 106-111Brazilian Journal of Oral Sciences; Vol. 14 No. 2 (2015): Apr./Jun.; 106-1111677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPporhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640822/8354Copyright (c) 2015 Janaína Cavalcante Lemos Lemos, Bruno César de Vasconcelos Gurgelhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLemos, Janaína Cavalcante LemosGurgel, Bruno César de Vasconcelos2023-10-02T13:26:42Zoai:ojs.periodicos.sbu.unicamp.br:article/8640822Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2023-10-02T13:26:42Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study
title Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study
spellingShingle Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study
Lemos, Janaína Cavalcante Lemos
Rats
Periodontal diseases
Th1 cells
Th2 cells
Cytokines
Immunohistochemistry
Odontologia
Periodontologia
title_short Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study
title_full Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study
title_fullStr Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study
title_full_unstemmed Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study
title_sort Th2 cells and the IFN-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study
author Lemos, Janaína Cavalcante Lemos
author_facet Lemos, Janaína Cavalcante Lemos
Gurgel, Bruno César de Vasconcelos
author_role author
author2 Gurgel, Bruno César de Vasconcelos
author2_role author
dc.contributor.author.fl_str_mv Lemos, Janaína Cavalcante Lemos
Gurgel, Bruno César de Vasconcelos
dc.subject.por.fl_str_mv Rats
Periodontal diseases
Th1 cells
Th2 cells
Cytokines
Immunohistochemistry
Odontologia
Periodontologia
topic Rats
Periodontal diseases
Th1 cells
Th2 cells
Cytokines
Immunohistochemistry
Odontologia
Periodontologia
description Aim: To evaluate the involvement of Th2 cells in different periods of the active phase of experimental periodontal disease and expression of the R1 subunit of the receptor for IFN-γ during the early and advanced progression of the disease. Methods: Experimental periodontitis was induced in 30 male Wistar rats by placing cotton ligatures around the mandibular first molars. The rats were then randomly assigned into two groups: G1=15 and G2=15, in group G1, ligatures were maintained for 2 days, a period that corresponds to the initial stage of periodontal disease in rats, in G2 ligatures were left for 15 days, a period that corresponds to the advanced stage of periodontal disease. The contra-lateral teeth served as controls (unligated). An immunohistochemical investigation of the gingival tissue was performed to detect the presence of the Th2 specific transcription factor (GATA3). Results: Light microscopy analysis revealed a decreased expression of GATA-3-positive cells when bone loss progressed. IFN-γ R1 was detected at an early stage during the active phase of disease, but the expression of positive cells remained unaltered during the remaining period of the study. Conclusions: These results indicate that the Th2 response may have a protective role during the pathogenesis of experimental periodontal disease, and that the IFN-γ R1 subunit may not be associated with periodontal disease progression.
publishDate 2015
dc.date.none.fl_str_mv 2015-05-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640822
url https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640822
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640822/8354
dc.rights.driver.fl_str_mv Copyright (c) 2015 Janaína Cavalcante Lemos Lemos, Bruno César de Vasconcelos Gurgel
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2015 Janaína Cavalcante Lemos Lemos, Bruno César de Vasconcelos Gurgel
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Estadual de Campinas
publisher.none.fl_str_mv Universidade Estadual de Campinas
dc.source.none.fl_str_mv Brazilian Journal of Oral Sciences; v. 14 n. 2 (2015): Apr./Jun.; 106-111
Brazilian Journal of Oral Sciences; Vol. 14 No. 2 (2015): Apr./Jun.; 106-111
1677-3225
reponame:Brazilian journal of oral sciences (Online)
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Brazilian journal of oral sciences (Online)
collection Brazilian journal of oral sciences (Online)
repository.name.fl_str_mv Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv brjorals@fop.unicamp.br||brjorals@fop.unicamp.br
_version_ 1800216397673922560