Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8657248 |
Resumo: | Aim: The aim of this study was to compare the microbial profile of subgingival sites in Periodontitis (Pd) patients and healthy ones. Methods: Eighteen patients with Pd and 18 gender-matched healthy controls were selected. Subgingival samples were collected from three types of sites: 1) healthy site of healthy subjects (probing pocket depth (PPD) ≤ 3mm, CG), 2) healthy site of Pd patients (PPD ≤ 3mm, PG-C) and 3) diseased site (PPD > 3mm) of the same Pd patients (PG-T). All sites were subjected to microbial analysis for the detection of 40 bacterial species by the "Checkerboard DNA-DNA hybridization" technique. Results: It was observed a great diversity of bacteria in all patients evaluated. The sites from the Pd groups (PG-T and PG-C) showed a higher overall count of the studied bacteria than those of the CG group, especially from Green, Orange, and Red complexes. Also, PG-T showed a higher prevalence of Red complex bacteria than CG. Individual pathogens, such as Porphyromonas gingivalis, Treponema denticola and Treponema socranskii were detected in higher levels and/or prevalence in Pd than in control patients. However, it was not observed any difference between PG-T and PG-C. Conclusion: Pd patients showed higher prevalence and counts of some putative periodontal bacteria, especially from the red complex, than control ones, regardless of the severity of their sites. |
id |
UNICAMP-8_410b7369d6363b6d8bdcc1583b212056 |
---|---|
oai_identifier_str |
oai:ojs.periodicos.sbu.unicamp.br:article/8657248 |
network_acronym_str |
UNICAMP-8 |
network_name_str |
Brazilian journal of oral sciences (Online) |
repository_id_str |
|
spelling |
Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patientsPeriodontitisBacteriaMolecular biology.Aim: The aim of this study was to compare the microbial profile of subgingival sites in Periodontitis (Pd) patients and healthy ones. Methods: Eighteen patients with Pd and 18 gender-matched healthy controls were selected. Subgingival samples were collected from three types of sites: 1) healthy site of healthy subjects (probing pocket depth (PPD) ≤ 3mm, CG), 2) healthy site of Pd patients (PPD ≤ 3mm, PG-C) and 3) diseased site (PPD > 3mm) of the same Pd patients (PG-T). All sites were subjected to microbial analysis for the detection of 40 bacterial species by the "Checkerboard DNA-DNA hybridization" technique. Results: It was observed a great diversity of bacteria in all patients evaluated. The sites from the Pd groups (PG-T and PG-C) showed a higher overall count of the studied bacteria than those of the CG group, especially from Green, Orange, and Red complexes. Also, PG-T showed a higher prevalence of Red complex bacteria than CG. Individual pathogens, such as Porphyromonas gingivalis, Treponema denticola and Treponema socranskii were detected in higher levels and/or prevalence in Pd than in control patients. However, it was not observed any difference between PG-T and PG-C. Conclusion: Pd patients showed higher prevalence and counts of some putative periodontal bacteria, especially from the red complex, than control ones, regardless of the severity of their sites.Universidade Estadual de Campinas2019-11-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionOriginal articleapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/865724810.20396/bjos.v18i0.8657248Brazilian Journal of Oral Sciences; v. 18 (2019): Continuous Publication; e191417Brazilian Journal of Oral Sciences; Vol. 18 (2019): Continuous Publication; e1914171677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8657248/21593Brazil; ContemporaryBarros, Carlos Eduardo Siqueira, Vivian Carvalho, DennisCanabarro, Antonio info:eu-repo/semantics/openAccess2020-12-22T01:33:53Zoai:ojs.periodicos.sbu.unicamp.br:article/8657248Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2020-12-22T01:33:53Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients |
title |
Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients |
spellingShingle |
Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients Barros, Carlos Eduardo Periodontitis Bacteria Molecular biology. |
title_short |
Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients |
title_full |
Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients |
title_fullStr |
Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients |
title_full_unstemmed |
Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients |
title_sort |
Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients |
author |
Barros, Carlos Eduardo |
author_facet |
Barros, Carlos Eduardo Siqueira, Vivian Carvalho, Dennis Canabarro, Antonio |
author_role |
author |
author2 |
Siqueira, Vivian Carvalho, Dennis Canabarro, Antonio |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Barros, Carlos Eduardo Siqueira, Vivian Carvalho, Dennis Canabarro, Antonio |
dc.subject.por.fl_str_mv |
Periodontitis Bacteria Molecular biology. |
topic |
Periodontitis Bacteria Molecular biology. |
description |
Aim: The aim of this study was to compare the microbial profile of subgingival sites in Periodontitis (Pd) patients and healthy ones. Methods: Eighteen patients with Pd and 18 gender-matched healthy controls were selected. Subgingival samples were collected from three types of sites: 1) healthy site of healthy subjects (probing pocket depth (PPD) ≤ 3mm, CG), 2) healthy site of Pd patients (PPD ≤ 3mm, PG-C) and 3) diseased site (PPD > 3mm) of the same Pd patients (PG-T). All sites were subjected to microbial analysis for the detection of 40 bacterial species by the "Checkerboard DNA-DNA hybridization" technique. Results: It was observed a great diversity of bacteria in all patients evaluated. The sites from the Pd groups (PG-T and PG-C) showed a higher overall count of the studied bacteria than those of the CG group, especially from Green, Orange, and Red complexes. Also, PG-T showed a higher prevalence of Red complex bacteria than CG. Individual pathogens, such as Porphyromonas gingivalis, Treponema denticola and Treponema socranskii were detected in higher levels and/or prevalence in Pd than in control patients. However, it was not observed any difference between PG-T and PG-C. Conclusion: Pd patients showed higher prevalence and counts of some putative periodontal bacteria, especially from the red complex, than control ones, regardless of the severity of their sites. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Original article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8657248 10.20396/bjos.v18i0.8657248 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8657248 |
identifier_str_mv |
10.20396/bjos.v18i0.8657248 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8657248/21593 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 18 (2019): Continuous Publication; e191417 Brazilian Journal of Oral Sciences; Vol. 18 (2019): Continuous Publication; e191417 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1800216402953502720 |