Bonding to wet or dry deproteinized dentin: microtensile bond strength and confocal laser micromorphology analysis

Detalhes bibliográficos
Autor(a) principal: Spazzin, Aloísio O.
Data de Publicação: 2015
Outros Autores: Galafassi, Daniel, Gonçalves, Luciano S., Moraes, Rafael R., Carlini-Júnior, Bruno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian journal of oral sciences (Online)
Texto Completo: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642041
Resumo: Aim: To investigate the influence of deproteinization and moisture condition (wet vs. dry) on the bond strength and micromorphology of resin-dentin bonding interfaces. Methods: Dentin surfaces were etched with 37% phosphoric acid for 15 s and rinsed with water. Four groups (n = 10) were tested: WET: dentin was left visibly moist; DRY: dentin was dried with compressed air; WET-D: dentin was deproteinized for 60 s using 10% NaOCl solution and left moist; DRY-D: dentin was deproteinized and dried. Prime&Bond 2.1 adhesive was applied and the teeth were restored with composite resin. Microtensile test was carried out after 24 h, and failure modes classified under magnification. Data were subjected to two-way ANOVA and Tukey’s test (P < 0.05). The bonding micromorphology was analyzed by confocal laser scanning microscopy. Results: The group DRY showed significantly lower bond strength (P < 0.05) than the other groups, which were similar to each other (P > 0.05). Adhesive failures were predominant. Analysis of micromorphology showed formation of a collagen-resin hybrid layer only for the non-deproteinized groups. Adhesive penetration into the dentinal tubules was deeper for the DRY-D compared to the WETD group. Conclusion: The bond strength was not dependent on the moisture condition and a more homogeneous hybridization was obtained when dentin was deproteinized
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spelling Bonding to wet or dry deproteinized dentin: microtensile bond strength and confocal laser micromorphology analysisBonding agentsDentinMicroscopyConfocalSodium hypochloriteBond strengthOdontologyAim: To investigate the influence of deproteinization and moisture condition (wet vs. dry) on the bond strength and micromorphology of resin-dentin bonding interfaces. Methods: Dentin surfaces were etched with 37% phosphoric acid for 15 s and rinsed with water. Four groups (n = 10) were tested: WET: dentin was left visibly moist; DRY: dentin was dried with compressed air; WET-D: dentin was deproteinized for 60 s using 10% NaOCl solution and left moist; DRY-D: dentin was deproteinized and dried. Prime&Bond 2.1 adhesive was applied and the teeth were restored with composite resin. Microtensile test was carried out after 24 h, and failure modes classified under magnification. Data were subjected to two-way ANOVA and Tukey’s test (P < 0.05). The bonding micromorphology was analyzed by confocal laser scanning microscopy. Results: The group DRY showed significantly lower bond strength (P < 0.05) than the other groups, which were similar to each other (P > 0.05). Adhesive failures were predominant. Analysis of micromorphology showed formation of a collagen-resin hybrid layer only for the non-deproteinized groups. Adhesive penetration into the dentinal tubules was deeper for the DRY-D compared to the WETD group. Conclusion: The bond strength was not dependent on the moisture condition and a more homogeneous hybridization was obtained when dentin was deproteinizedUniversidade Estadual de Campinas2015-12-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/864204110.20396/bjos.v8i4.8642041Brazilian Journal of Oral Sciences; v. 8 n. 4 (2009): Oct./Dec.; 181-184Brazilian Journal of Oral Sciences; Vol. 8 No. 4 (2009): Oct./Dec.; 181-1841677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642041/9536Spazzin, Aloísio O.Galafassi, DanielGonçalves, Luciano S.Moraes, Rafael R.Carlini-Júnior, Brunoinfo:eu-repo/semantics/openAccess2016-02-25T09:13:28Zoai:ojs.periodicos.sbu.unicamp.br:article/8642041Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2016-02-25T09:13:28Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Bonding to wet or dry deproteinized dentin: microtensile bond strength and confocal laser micromorphology analysis
title Bonding to wet or dry deproteinized dentin: microtensile bond strength and confocal laser micromorphology analysis
spellingShingle Bonding to wet or dry deproteinized dentin: microtensile bond strength and confocal laser micromorphology analysis
Spazzin, Aloísio O.
Bonding agents
Dentin
Microscopy
Confocal
Sodium hypochlorite
Bond strength
Odontology
title_short Bonding to wet or dry deproteinized dentin: microtensile bond strength and confocal laser micromorphology analysis
title_full Bonding to wet or dry deproteinized dentin: microtensile bond strength and confocal laser micromorphology analysis
title_fullStr Bonding to wet or dry deproteinized dentin: microtensile bond strength and confocal laser micromorphology analysis
title_full_unstemmed Bonding to wet or dry deproteinized dentin: microtensile bond strength and confocal laser micromorphology analysis
title_sort Bonding to wet or dry deproteinized dentin: microtensile bond strength and confocal laser micromorphology analysis
author Spazzin, Aloísio O.
author_facet Spazzin, Aloísio O.
Galafassi, Daniel
Gonçalves, Luciano S.
Moraes, Rafael R.
Carlini-Júnior, Bruno
author_role author
author2 Galafassi, Daniel
Gonçalves, Luciano S.
Moraes, Rafael R.
Carlini-Júnior, Bruno
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Spazzin, Aloísio O.
Galafassi, Daniel
Gonçalves, Luciano S.
Moraes, Rafael R.
Carlini-Júnior, Bruno
dc.subject.por.fl_str_mv Bonding agents
Dentin
Microscopy
Confocal
Sodium hypochlorite
Bond strength
Odontology
topic Bonding agents
Dentin
Microscopy
Confocal
Sodium hypochlorite
Bond strength
Odontology
description Aim: To investigate the influence of deproteinization and moisture condition (wet vs. dry) on the bond strength and micromorphology of resin-dentin bonding interfaces. Methods: Dentin surfaces were etched with 37% phosphoric acid for 15 s and rinsed with water. Four groups (n = 10) were tested: WET: dentin was left visibly moist; DRY: dentin was dried with compressed air; WET-D: dentin was deproteinized for 60 s using 10% NaOCl solution and left moist; DRY-D: dentin was deproteinized and dried. Prime&Bond 2.1 adhesive was applied and the teeth were restored with composite resin. Microtensile test was carried out after 24 h, and failure modes classified under magnification. Data were subjected to two-way ANOVA and Tukey’s test (P < 0.05). The bonding micromorphology was analyzed by confocal laser scanning microscopy. Results: The group DRY showed significantly lower bond strength (P < 0.05) than the other groups, which were similar to each other (P > 0.05). Adhesive failures were predominant. Analysis of micromorphology showed formation of a collagen-resin hybrid layer only for the non-deproteinized groups. Adhesive penetration into the dentinal tubules was deeper for the DRY-D compared to the WETD group. Conclusion: The bond strength was not dependent on the moisture condition and a more homogeneous hybridization was obtained when dentin was deproteinized
publishDate 2015
dc.date.none.fl_str_mv 2015-12-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642041
10.20396/bjos.v8i4.8642041
url https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642041
identifier_str_mv 10.20396/bjos.v8i4.8642041
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642041/9536
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Campinas
publisher.none.fl_str_mv Universidade Estadual de Campinas
dc.source.none.fl_str_mv Brazilian Journal of Oral Sciences; v. 8 n. 4 (2009): Oct./Dec.; 181-184
Brazilian Journal of Oral Sciences; Vol. 8 No. 4 (2009): Oct./Dec.; 181-184
1677-3225
reponame:Brazilian journal of oral sciences (Online)
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Brazilian journal of oral sciences (Online)
collection Brazilian journal of oral sciences (Online)
repository.name.fl_str_mv Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv brjorals@fop.unicamp.br||brjorals@fop.unicamp.br
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