An in vitro microbial model for producing caries-like lesions on enamel
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642998 |
Resumo: | This study aimed to develop a low cost in vitro viable microbiological model to produce biofilms to be used in dental researches. Single and multi-species biofilms of S. mutans, S. sobrinus, S. mitis, S. salivarius, S. cricetus and S. sanguinis were grown on bovine enamel slabs during 10 days, in a sterile brain-heart infusion broth, containing 5% sucrose and incubated at 37ºC in an atmosphere of 10% CO2 . The slabs were transferred to a fresh medium at every 6, 12 or 24 hours. After the experimental period, enamel volume percent mineral was determined by cross-sectional microhardness. Caries-like lesions were found in all bacterial groups when compared with the control group. No statistical significant differences were found between S. mutans and S. sobrinus with respect of their cariogenicity or among the periods of medium change. However, it was found a statistical significant difference among the cariogenicity of S. salivarius and S. sanguinis (ANOVA followed by Tukey test). This model has successfully developed caries-like lesion on enamel and the medium can be changed at every 24 hours utilizing either S. mutans or S. sobrinus. |
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Brazilian journal of oral sciences (Online) |
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An in vitro microbial model for producing caries-like lesions on enamelBiofilms. Microbiological. Enamel. S. mutans. S. sobrinusOdontologiaThis study aimed to develop a low cost in vitro viable microbiological model to produce biofilms to be used in dental researches. Single and multi-species biofilms of S. mutans, S. sobrinus, S. mitis, S. salivarius, S. cricetus and S. sanguinis were grown on bovine enamel slabs during 10 days, in a sterile brain-heart infusion broth, containing 5% sucrose and incubated at 37ºC in an atmosphere of 10% CO2 . The slabs were transferred to a fresh medium at every 6, 12 or 24 hours. After the experimental period, enamel volume percent mineral was determined by cross-sectional microhardness. Caries-like lesions were found in all bacterial groups when compared with the control group. No statistical significant differences were found between S. mutans and S. sobrinus with respect of their cariogenicity or among the periods of medium change. However, it was found a statistical significant difference among the cariogenicity of S. salivarius and S. sanguinis (ANOVA followed by Tukey test). This model has successfully developed caries-like lesion on enamel and the medium can be changed at every 24 hours utilizing either S. mutans or S. sobrinus.Universidade Estadual de Campinas2016-01-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/864299810.20396/bjos.v6i22.8642998Brazilian Journal of Oral Sciences; v. 6 n. 22 (2007): Ju./Sept; 1392-1396Brazilian Journal of Oral Sciences; Vol. 6 No. 22 (2007): Ju./Sept; 1392-13961677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642998/10553Steiner-Oliveira, CarolinaMaciel, Fernando A.Rodrigues, Lidiany K.A.Napimoga, Marcelo H.Pimenta, Luiz A.F.Höfling, José F.Gonçalves, Reginaldo B.info:eu-repo/semantics/openAccess2016-02-25T09:25:40Zoai:ojs.periodicos.sbu.unicamp.br:article/8642998Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2016-02-25T09:25:40Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
An in vitro microbial model for producing caries-like lesions on enamel |
title |
An in vitro microbial model for producing caries-like lesions on enamel |
spellingShingle |
An in vitro microbial model for producing caries-like lesions on enamel Steiner-Oliveira, Carolina Biofilms. Microbiological. Enamel. S. mutans. S. sobrinus Odontologia |
title_short |
An in vitro microbial model for producing caries-like lesions on enamel |
title_full |
An in vitro microbial model for producing caries-like lesions on enamel |
title_fullStr |
An in vitro microbial model for producing caries-like lesions on enamel |
title_full_unstemmed |
An in vitro microbial model for producing caries-like lesions on enamel |
title_sort |
An in vitro microbial model for producing caries-like lesions on enamel |
author |
Steiner-Oliveira, Carolina |
author_facet |
Steiner-Oliveira, Carolina Maciel, Fernando A. Rodrigues, Lidiany K.A. Napimoga, Marcelo H. Pimenta, Luiz A.F. Höfling, José F. Gonçalves, Reginaldo B. |
author_role |
author |
author2 |
Maciel, Fernando A. Rodrigues, Lidiany K.A. Napimoga, Marcelo H. Pimenta, Luiz A.F. Höfling, José F. Gonçalves, Reginaldo B. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Steiner-Oliveira, Carolina Maciel, Fernando A. Rodrigues, Lidiany K.A. Napimoga, Marcelo H. Pimenta, Luiz A.F. Höfling, José F. Gonçalves, Reginaldo B. |
dc.subject.por.fl_str_mv |
Biofilms. Microbiological. Enamel. S. mutans. S. sobrinus Odontologia |
topic |
Biofilms. Microbiological. Enamel. S. mutans. S. sobrinus Odontologia |
description |
This study aimed to develop a low cost in vitro viable microbiological model to produce biofilms to be used in dental researches. Single and multi-species biofilms of S. mutans, S. sobrinus, S. mitis, S. salivarius, S. cricetus and S. sanguinis were grown on bovine enamel slabs during 10 days, in a sterile brain-heart infusion broth, containing 5% sucrose and incubated at 37ºC in an atmosphere of 10% CO2 . The slabs were transferred to a fresh medium at every 6, 12 or 24 hours. After the experimental period, enamel volume percent mineral was determined by cross-sectional microhardness. Caries-like lesions were found in all bacterial groups when compared with the control group. No statistical significant differences were found between S. mutans and S. sobrinus with respect of their cariogenicity or among the periods of medium change. However, it was found a statistical significant difference among the cariogenicity of S. salivarius and S. sanguinis (ANOVA followed by Tukey test). This model has successfully developed caries-like lesion on enamel and the medium can be changed at every 24 hours utilizing either S. mutans or S. sobrinus. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642998 10.20396/bjos.v6i22.8642998 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642998 |
identifier_str_mv |
10.20396/bjos.v6i22.8642998 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642998/10553 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 6 n. 22 (2007): Ju./Sept; 1392-1396 Brazilian Journal of Oral Sciences; Vol. 6 No. 22 (2007): Ju./Sept; 1392-1396 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1800216401196089344 |