Comparative in vitro analysis of the antifungal activity of different calcium silicate-based endodontic sealers

• Autor(a) principal: Moreira, Luiz Felipe Nunes
• Data de Publicação: 2024
• Outros Autores: Peña-Bengoa, Fernando, Niklander, Sven Eric, Bueno, Carlos Eduardo da Silveira, Martin, Alexandre Sigrist de, Rocha, Daniel Guimarães Pedro
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian journal of oral sciences (Online)
• DOI: 10.20396/bjos.v23i00.8673355
• Texto Completo: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8673355
• topic: Endodontics; Bacteria; Candida albicans; Root canal filling materials

Aim: This study aimed to perform an in vitro comparative analysis of the antifungal activity of different calcium silicate-based endodontic sealers against three fungal species. Methods: The antifungal properties of three calcium silicate-based sealers were tested: Bio-C Sealer, Cambiar a Sealer Plus BC, and MTA-Fillapex. Two commonly used sealers were used as controls: AH Plus and Endomethasone. An agar diffusion test was performed to analyze the antifungal activity of the sealers against Candida albicans, Candida glabrata, Candida tropicalis, and a mixed microbial culture medium. The results were analyzed using ANOVA (p <0.05). Results: Endomethasone exhibited the highest inhibition against all strains examined, maintaining a consistent level of inhibition throughout 7 days. MTA-Fillapex demonstrated the best performance among the calcium silicate-based sealers for the three fungal species (p < 0.05), maintaining stable values over the 7 days, surpassing that of Endomethasone. Nevertheless, MTA-Fillapex only exhibited antimicrobial effect against the mixed culture for the first 24 hours, and no antimicrobial activity was observed at 48 hours, being surpassed by all tested sealers (p < 0.05). Conclusion: Of all silicate-based sealers tested, only MTA-Fillapex exhibited promising antifungal activity. Nevertheless, care must be taken when extrapolating these results, as MTA-Fillapex exhibited poor antimicrobial activity when tested in mixed microbial cultures.