A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641064 |
Resumo: | Aim: To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic solution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis employing 3T3 cells. Methods: Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24h, at 37°C with 5% CO2 , and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test), and total cell density (crystal violet dye exclusion test). Results from each test were compared by one-way analysis of variance (ANOVA). Results: Statistical analysis showed that whole milk and HBSS were the most effective media in maintaining cell viability at all tested times (p<0.05). Isotonic and tap water showed the highest cytotoxicity effects. Conclusions: This study shows that whole milk and HBSS are more efficient in maintaining the viability of 3T3 fibroblasts as demonstrated by three different cell viability tests. |
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oai:ojs.periodicos.sbu.unicamp.br:article/8641064 |
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UNICAMP-8 |
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Brazilian journal of oral sciences (Online) |
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A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teethTooth avulsionFibroblastsCell viabilityOdontologiaAim: To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic solution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis employing 3T3 cells. Methods: Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24h, at 37°C with 5% CO2 , and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test), and total cell density (crystal violet dye exclusion test). Results from each test were compared by one-way analysis of variance (ANOVA). Results: Statistical analysis showed that whole milk and HBSS were the most effective media in maintaining cell viability at all tested times (p<0.05). Isotonic and tap water showed the highest cytotoxicity effects. Conclusions: This study shows that whole milk and HBSS are more efficient in maintaining the viability of 3T3 fibroblasts as demonstrated by three different cell viability tests.Universidade Estadual de Campinas2015-10-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641064Brazilian Journal of Oral Sciences; v. 12 n. 2 (2013): Abr./Jun.; 90-94Brazilian Journal of Oral Sciences; Vol. 12 No. 2 (2013): Abr./Jun.; 90-941677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPporhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641064/8579Copyright (c) 2015 Emmanuel João Nogueira Leal Silva, Carolina Bluzarca Rollemberg, Tauby de Souza Coutinho Filho, Renato Liess Krebs, Alexandre Augusto Zaiahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Emmanuel João Nogueira LealRollemberg, Carolina BluzarcaCoutinho Filho, Tauby de SouzaKrebs, Renato LiessZaia, Alexandre Augusto2023-09-29T12:45:43Zoai:ojs.periodicos.sbu.unicamp.br:article/8641064Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2023-09-29T12:45:43Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth |
title |
A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth |
spellingShingle |
A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth Silva, Emmanuel João Nogueira Leal Tooth avulsion Fibroblasts Cell viability Odontologia |
title_short |
A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth |
title_full |
A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth |
title_fullStr |
A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth |
title_full_unstemmed |
A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth |
title_sort |
A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth |
author |
Silva, Emmanuel João Nogueira Leal |
author_facet |
Silva, Emmanuel João Nogueira Leal Rollemberg, Carolina Bluzarca Coutinho Filho, Tauby de Souza Krebs, Renato Liess Zaia, Alexandre Augusto |
author_role |
author |
author2 |
Rollemberg, Carolina Bluzarca Coutinho Filho, Tauby de Souza Krebs, Renato Liess Zaia, Alexandre Augusto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Emmanuel João Nogueira Leal Rollemberg, Carolina Bluzarca Coutinho Filho, Tauby de Souza Krebs, Renato Liess Zaia, Alexandre Augusto |
dc.subject.por.fl_str_mv |
Tooth avulsion Fibroblasts Cell viability Odontologia |
topic |
Tooth avulsion Fibroblasts Cell viability Odontologia |
description |
Aim: To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic solution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis employing 3T3 cells. Methods: Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24h, at 37°C with 5% CO2 , and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test), and total cell density (crystal violet dye exclusion test). Results from each test were compared by one-way analysis of variance (ANOVA). Results: Statistical analysis showed that whole milk and HBSS were the most effective media in maintaining cell viability at all tested times (p<0.05). Isotonic and tap water showed the highest cytotoxicity effects. Conclusions: This study shows that whole milk and HBSS are more efficient in maintaining the viability of 3T3 fibroblasts as demonstrated by three different cell viability tests. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641064 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641064 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641064/8579 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 12 n. 2 (2013): Abr./Jun.; 90-94 Brazilian Journal of Oral Sciences; Vol. 12 No. 2 (2013): Abr./Jun.; 90-94 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1800216398252736512 |